The genomic analysis of a wide peach germplasm collection revealed genetic relationships between European landraces and American ferals and landraces
The advance of next generation sequencing (NGS) technologies allows the implementation of high-throughput genotyping at a reasonable cost. This open the possibility to learn more about phylogenetic relationships, genetic variability, and the development of new genetic markers for breeding purposes....
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
ISHS
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/13837 https://www.actahort.org/books/1352/1352_11.htm https://doi.org/10.17660/ActaHortic.2022.1352.11 |
| _version_ | 1855485236042792960 |
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| author | Aballay, Maximiliano Martín Ksouri, Najla Valentini, Gabriel Hugo Puebla, Andrea Fabiana Gogorcena Aoiz, Yolanda Sanchez, Gerardo |
| author_browse | Aballay, Maximiliano Martín Gogorcena Aoiz, Yolanda Ksouri, Najla Puebla, Andrea Fabiana Sanchez, Gerardo Valentini, Gabriel Hugo |
| author_facet | Aballay, Maximiliano Martín Ksouri, Najla Valentini, Gabriel Hugo Puebla, Andrea Fabiana Gogorcena Aoiz, Yolanda Sanchez, Gerardo |
| author_sort | Aballay, Maximiliano Martín |
| collection | INTA Digital |
| description | The advance of next generation sequencing (NGS) technologies allows the implementation of high-throughput genotyping at a reasonable cost. This open the possibility to learn more about phylogenetic relationships, genetic variability, and the development of new genetic markers for breeding purposes. In this work a previous developed ddRAD-seq platform was applied for the genotyping of two peach collections curated at the EEA San Pedro (237 genotypes) and the EE Aula Dei (89 genotypes). Here we present a joint analysis of a total of 798×106 of paired-end (2×250 bp) reads belonging to 326 genotypes (2,45M reads genotype-1). A total of 564,382 SNPs were discovered and a reduced data set composed of 10,576 SNP (MAF >1% and missing data <10%) was used in a combination of analyses (principal component analysis, population structure and phylogeny). The results showed that the ancestral germplasms differ largely from modern peach cultivars. In addition, genetic relationships between American landraces (North American and Bolivian), American ferals (Argentinian) and European landraces (Spanish and Italian) were revealed suggesting a common origin which probably reflect the introduction of germplasm by the Spaniards during the colonization period. These results with some outstanding traits of ancestral genotypes (fruit quality, high yield/vigour, pathogen resistance, thermal requirements, etc.) encourage the usage of this germplasm on breeding programs for the development of new varieties with agricultural impact. |
| format | Artículo |
| id | INTA13837 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | ISHS |
| publisherStr | ISHS |
| record_format | dspace |
| spelling | INTA138372023-01-06T11:00:38Z The genomic analysis of a wide peach germplasm collection revealed genetic relationships between European landraces and American ferals and landraces Aballay, Maximiliano Martín Ksouri, Najla Valentini, Gabriel Hugo Puebla, Andrea Fabiana Gogorcena Aoiz, Yolanda Sanchez, Gerardo Prunus persica Filogenética Variación Genética Secuenciación de Alto Rendimiento Fitomejoramiento Durazno Biotecnología Vegetal Phylogenetics Genetic Variation High-throughput Sequencing Plant Breeding Peaches Plant Biotechnology NGS Next Generation Sequencing The advance of next generation sequencing (NGS) technologies allows the implementation of high-throughput genotyping at a reasonable cost. This open the possibility to learn more about phylogenetic relationships, genetic variability, and the development of new genetic markers for breeding purposes. In this work a previous developed ddRAD-seq platform was applied for the genotyping of two peach collections curated at the EEA San Pedro (237 genotypes) and the EE Aula Dei (89 genotypes). Here we present a joint analysis of a total of 798×106 of paired-end (2×250 bp) reads belonging to 326 genotypes (2,45M reads genotype-1). A total of 564,382 SNPs were discovered and a reduced data set composed of 10,576 SNP (MAF >1% and missing data <10%) was used in a combination of analyses (principal component analysis, population structure and phylogeny). The results showed that the ancestral germplasms differ largely from modern peach cultivars. In addition, genetic relationships between American landraces (North American and Bolivian), American ferals (Argentinian) and European landraces (Spanish and Italian) were revealed suggesting a common origin which probably reflect the introduction of germplasm by the Spaniards during the colonization period. These results with some outstanding traits of ancestral genotypes (fruit quality, high yield/vigour, pathogen resistance, thermal requirements, etc.) encourage the usage of this germplasm on breeding programs for the development of new varieties with agricultural impact. EEA San Pedro Fil: Aballay, Maximiliano Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Ksouri, Najla. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental Aula Dei; España Fil: Valentini, Gabriel Hugo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Puebla, Andrea Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Fil: Gogorcena Aoiz, Yolanda. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental Aula Dei; España Fil: Sánchez, Gerardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina 2023-01-06T10:48:47Z 2023-01-06T10:48:47Z 2022-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/draft http://hdl.handle.net/20.500.12123/13837 https://www.actahort.org/books/1352/1352_11.htm 978-94-62613-52-2 2406-6168 https://doi.org/10.17660/ActaHortic.2022.1352.11 eng info:eu-repograntAgreement/INTA/2019-PE-E6-I114-001/2019-PE-E6-I114-001/AR./Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada. info:eu-repo/semantics/restrictedAccess application/pdf ISHS Acta horticulturae 1352 : 81-88. (Dec. 2022) |
| spellingShingle | Prunus persica Filogenética Variación Genética Secuenciación de Alto Rendimiento Fitomejoramiento Durazno Biotecnología Vegetal Phylogenetics Genetic Variation High-throughput Sequencing Plant Breeding Peaches Plant Biotechnology NGS Next Generation Sequencing Aballay, Maximiliano Martín Ksouri, Najla Valentini, Gabriel Hugo Puebla, Andrea Fabiana Gogorcena Aoiz, Yolanda Sanchez, Gerardo The genomic analysis of a wide peach germplasm collection revealed genetic relationships between European landraces and American ferals and landraces |
| title | The genomic analysis of a wide peach germplasm collection revealed genetic relationships between European landraces and American ferals and landraces |
| title_full | The genomic analysis of a wide peach germplasm collection revealed genetic relationships between European landraces and American ferals and landraces |
| title_fullStr | The genomic analysis of a wide peach germplasm collection revealed genetic relationships between European landraces and American ferals and landraces |
| title_full_unstemmed | The genomic analysis of a wide peach germplasm collection revealed genetic relationships between European landraces and American ferals and landraces |
| title_short | The genomic analysis of a wide peach germplasm collection revealed genetic relationships between European landraces and American ferals and landraces |
| title_sort | genomic analysis of a wide peach germplasm collection revealed genetic relationships between european landraces and american ferals and landraces |
| topic | Prunus persica Filogenética Variación Genética Secuenciación de Alto Rendimiento Fitomejoramiento Durazno Biotecnología Vegetal Phylogenetics Genetic Variation High-throughput Sequencing Plant Breeding Peaches Plant Biotechnology NGS Next Generation Sequencing |
| url | http://hdl.handle.net/20.500.12123/13837 https://www.actahort.org/books/1352/1352_11.htm https://doi.org/10.17660/ActaHortic.2022.1352.11 |
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