Determination of alkaloids in onion nectar by micellar electrokinetic chromatography
Nectar is the most important floral reward offered by plants to insects. Minor components such as alkaloid compounds in nectar affect bee foraging, with great influence in seed production. CE is an advantageous tool for the analysis of unexplored samples such as onion nectar due to the limited amoun...
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| Format: | Artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1379 http://onlinelibrary.wiley.com/doi/10.1002/elps.201600060/pdf |
| _version_ | 1855482877978869760 |
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| author | Soto Vargas, Verónica Carolina Jofre, Viviana Patricia Galmarini, Claudio Romulo Silva, Maria Fernanda |
| author_browse | Galmarini, Claudio Romulo Jofre, Viviana Patricia Silva, Maria Fernanda Soto Vargas, Verónica Carolina |
| author_facet | Soto Vargas, Verónica Carolina Jofre, Viviana Patricia Galmarini, Claudio Romulo Silva, Maria Fernanda |
| author_sort | Soto Vargas, Verónica Carolina |
| collection | INTA Digital |
| description | Nectar is the most important floral reward offered by plants to insects. Minor components such as alkaloid compounds in nectar affect bee foraging, with great influence in seed production. CE is an advantageous tool for the analysis of unexplored samples such as onion nectar due to the limited amounts of samples. Considering the importance of these compounds, a simultaneous determination of nicotine, theophylline, theobromine, caffeine, harmaline, piperine in onion nectar byMEKC-UV is herein reported. The extraction of alkaloid compounds in nectar was performed by SPE using a homemade miniaturized column (C18). Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire optimum conditions. Under the proposed conditions, the analytes can be separated within 15 min in a 50 cm effective length capillary (75 _m id) at a separation voltage of 20 kV in 20 mmol/L sodium tretraborate, 100 mmol/L SDS. The amount of sample requirement was reduced up to 2000 times, when compared to traditional methods, reaching limits of detection as low as 0.0153 ng/L. For the first time, this study demonstrates that there are marked qualitative and quantitative differences in nectar alkaloids between open pollinated and male sterile lines (MSLs) and also within MSLs. |
| format | Artículo |
| id | INTA1379 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA13792018-02-15T17:55:36Z Determination of alkaloids in onion nectar by micellar electrokinetic chromatography Soto Vargas, Verónica Carolina Jofre, Viviana Patricia Galmarini, Claudio Romulo Silva, Maria Fernanda Cebolla Alcaloides Néctar Cromatografía Onion Alkaloids Chromatography Nectar is the most important floral reward offered by plants to insects. Minor components such as alkaloid compounds in nectar affect bee foraging, with great influence in seed production. CE is an advantageous tool for the analysis of unexplored samples such as onion nectar due to the limited amounts of samples. Considering the importance of these compounds, a simultaneous determination of nicotine, theophylline, theobromine, caffeine, harmaline, piperine in onion nectar byMEKC-UV is herein reported. The extraction of alkaloid compounds in nectar was performed by SPE using a homemade miniaturized column (C18). Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire optimum conditions. Under the proposed conditions, the analytes can be separated within 15 min in a 50 cm effective length capillary (75 _m id) at a separation voltage of 20 kV in 20 mmol/L sodium tretraborate, 100 mmol/L SDS. The amount of sample requirement was reduced up to 2000 times, when compared to traditional methods, reaching limits of detection as low as 0.0153 ng/L. For the first time, this study demonstrates that there are marked qualitative and quantitative differences in nectar alkaloids between open pollinated and male sterile lines (MSLs) and also within MSLs. EEA Mendoza Fil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Jofre, Viviana Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina 2017-10-02T17:11:26Z 2017-10-02T17:11:26Z 2016 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:ar-repo/semantics/artículo http://hdl.handle.net/20.500.12123/1379 http://onlinelibrary.wiley.com/doi/10.1002/elps.201600060/pdf 0173-0835 (Print) 1522-2683 (Online) DOI: 10.1002/elps.201600060 eng info:eu-repo/semantics/restrictedAccess application/pdf Electrophoresis 37 (13) : 1909–1915. (July 2016) |
| spellingShingle | Cebolla Alcaloides Néctar Cromatografía Onion Alkaloids Chromatography Soto Vargas, Verónica Carolina Jofre, Viviana Patricia Galmarini, Claudio Romulo Silva, Maria Fernanda Determination of alkaloids in onion nectar by micellar electrokinetic chromatography |
| title | Determination of alkaloids in onion nectar by micellar electrokinetic chromatography |
| title_full | Determination of alkaloids in onion nectar by micellar electrokinetic chromatography |
| title_fullStr | Determination of alkaloids in onion nectar by micellar electrokinetic chromatography |
| title_full_unstemmed | Determination of alkaloids in onion nectar by micellar electrokinetic chromatography |
| title_short | Determination of alkaloids in onion nectar by micellar electrokinetic chromatography |
| title_sort | determination of alkaloids in onion nectar by micellar electrokinetic chromatography |
| topic | Cebolla Alcaloides Néctar Cromatografía Onion Alkaloids Chromatography |
| url | http://hdl.handle.net/20.500.12123/1379 http://onlinelibrary.wiley.com/doi/10.1002/elps.201600060/pdf |
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