Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina
Sarcocystis spp. are intracellular protozoan parasites with an obligatory heteroxenous life cycle. The objective of this study is to identify Sarcocystis spp. in wild boar muscles from Argentina by light and transmission electron microscopy and molecular characterization. Muscle samples from diaphra...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Español |
| Publicado: |
Springer
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/13787 https://link.springer.com/article/10.1007/s00436-022-07743-w https://doi.org/10.1007/s00436-022-07743-w |
| _version_ | 1855037002277191680 |
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| author | Helman, Elisa Dellarupe, Andrea Cifuentes, Sabrina Chang Reissig, Elizabeth Moré, Gastón |
| author_browse | Chang Reissig, Elizabeth Cifuentes, Sabrina Dellarupe, Andrea Helman, Elisa Moré, Gastón |
| author_facet | Helman, Elisa Dellarupe, Andrea Cifuentes, Sabrina Chang Reissig, Elizabeth Moré, Gastón |
| author_sort | Helman, Elisa |
| collection | INTA Digital |
| description | Sarcocystis spp. are intracellular protozoan parasites with an obligatory heteroxenous life cycle. The objective of this study is to identify Sarcocystis spp. in wild boar muscles from Argentina by light and transmission electron microscopy and molecular characterization. Muscle samples from diaphragm, tongue, masseter, intercostals, heart, and forelimbs of 240 wild boars were analyzed. Of the animals, 48.3% (116/240) were positive for sarcocysts by light microscopy, whereas 45.8% (110/240) were positive for Sarcocystis spp. by PCR targeting 18S rRNA fragment. These samples were subjected to a specific PCR for S. suihominis coxI gene, 3.6% (4/110) of which were weak positives. Unfortunately, sequence analysis was inconclusive. This could be related to a potentially low S. suihominis cyst load in the samples, or to an incomplete primer matching with the South American S. suihominis sequences. Seventeen individual sarcocysts were positive by PCR for the 18S rRNA fragment, whose sequences showed 99.75–100% identity with each other and with previously reported S.miescheriana sequences. A total of 21 cysts collected from 11 muscle samples and analyzed by TEM presented a cyst wall type compatible with S. miescheriana, and one cyst presented an ultrastructure compatible with S. suihominis. The latter came from a sample that also contained S. miescheriana cysts, indicating that the animal was co-infected. This is the first study that provides infection rates and describes and identifies morphological and molecular features of Sarcocystis spp. cysts in wild boars from South America. |
| format | info:ar-repo/semantics/artículo |
| id | INTA13787 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Español |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA137872023-01-03T10:00:19Z Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina Helman, Elisa Dellarupe, Andrea Cifuentes, Sabrina Chang Reissig, Elizabeth Moré, Gastón Sarcocystis Sarcocystis miescheriana Parásitos Enfermedades Parasitarias Parasites Parasitoses Wild Boar Sus scrofa Jabalí Argentina Sarcocystis spp. are intracellular protozoan parasites with an obligatory heteroxenous life cycle. The objective of this study is to identify Sarcocystis spp. in wild boar muscles from Argentina by light and transmission electron microscopy and molecular characterization. Muscle samples from diaphragm, tongue, masseter, intercostals, heart, and forelimbs of 240 wild boars were analyzed. Of the animals, 48.3% (116/240) were positive for sarcocysts by light microscopy, whereas 45.8% (110/240) were positive for Sarcocystis spp. by PCR targeting 18S rRNA fragment. These samples were subjected to a specific PCR for S. suihominis coxI gene, 3.6% (4/110) of which were weak positives. Unfortunately, sequence analysis was inconclusive. This could be related to a potentially low S. suihominis cyst load in the samples, or to an incomplete primer matching with the South American S. suihominis sequences. Seventeen individual sarcocysts were positive by PCR for the 18S rRNA fragment, whose sequences showed 99.75–100% identity with each other and with previously reported S.miescheriana sequences. A total of 21 cysts collected from 11 muscle samples and analyzed by TEM presented a cyst wall type compatible with S. miescheriana, and one cyst presented an ultrastructure compatible with S. suihominis. The latter came from a sample that also contained S. miescheriana cysts, indicating that the animal was co-infected. This is the first study that provides infection rates and describes and identifies morphological and molecular features of Sarcocystis spp. cysts in wild boars from South America. Estación Experimental Agropecuaria Bariloche Fil: Helman, Elisa. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina Fil: Helman, Elisa. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina Fil: Dellarupe, Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina Fil: Dellarupe, Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina Fil: Cifuentes, Sabrina. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina Fil: Chang Reissig, Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina Fil: Chang Reissig, Elizabeth. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina Fil: Moré, Gastón. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina 2023-01-03T09:54:24Z 2023-01-03T09:54:24Z 2022-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13787 https://link.springer.com/article/10.1007/s00436-022-07743-w 0932-0113 1432-1955 https://doi.org/10.1007/s00436-022-07743-w spa info:eu-repo/semantics/restrictedAccess application/pdf Springer Parasitology Research (Published: 06 December 2022) |
| spellingShingle | Sarcocystis Sarcocystis miescheriana Parásitos Enfermedades Parasitarias Parasites Parasitoses Wild Boar Sus scrofa Jabalí Argentina Helman, Elisa Dellarupe, Andrea Cifuentes, Sabrina Chang Reissig, Elizabeth Moré, Gastón Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina |
| title | Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina |
| title_full | Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina |
| title_fullStr | Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina |
| title_full_unstemmed | Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina |
| title_short | Identification of Sarcocystis spp. in wild boars (Sus scrofa) from Argentina |
| title_sort | identification of sarcocystis spp in wild boars sus scrofa from argentina |
| topic | Sarcocystis Sarcocystis miescheriana Parásitos Enfermedades Parasitarias Parasites Parasitoses Wild Boar Sus scrofa Jabalí Argentina |
| url | http://hdl.handle.net/20.500.12123/13787 https://link.springer.com/article/10.1007/s00436-022-07743-w https://doi.org/10.1007/s00436-022-07743-w |
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