Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production
In this study, a simple and solvent-free method using headspace solid-phase microextraction (SPME) and gas chromatography–mass spectrometry (GC–MS) was developed for determination of the volatile compounds from fresh flowers and nectar of male sterile and fertile lines of Allium cepa L. The SPME par...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1375 http://www.sciencedirect.com/science/article/pii/S0026265X15000788 https://doi.org/10.1016/j.microc.2015.04.017 |
| _version_ | 1855482877392715776 |
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| author | Soto Vargas, Verónica Carolina Maldonado, Irma Beatriz Jofre, Viviana Patricia Galmarini, Claudio Romulo Silva, Maria Fernanda |
| author_browse | Galmarini, Claudio Romulo Jofre, Viviana Patricia Maldonado, Irma Beatriz Silva, Maria Fernanda Soto Vargas, Verónica Carolina |
| author_facet | Soto Vargas, Verónica Carolina Maldonado, Irma Beatriz Jofre, Viviana Patricia Galmarini, Claudio Romulo Silva, Maria Fernanda |
| author_sort | Soto Vargas, Verónica Carolina |
| collection | INTA Digital |
| description | In this study, a simple and solvent-free method using headspace solid-phase microextraction (SPME) and gas chromatography–mass spectrometry (GC–MS) was developed for determination of the volatile compounds from fresh flowers and nectar of male sterile and fertile lines of Allium cepa L. The SPME parameters were studied; the optimum conditions of 85 μm carboxen/polydimethylsiloxane (CAR/PDMS), extraction temperature of 30 °C and extraction time of 30 min were obtained. The analytical method was applied to characterize different onion lines according to the chemical composition of the volatile fraction of nectar and flowers. On the other hand, it was determined which odor components contribute to the pollination of onions lines and relate the content of specific analytes with foraging behavior and seed production. More than 90 compounds were identified. The samples studied showed differences in the volatile profiles of flowers and nectar among the different lines. The results demonstrated that headspace SPME–GCMS is a simple, rapid and solvent-free method suitable for analysis of volatile compounds emitted from onion plants. Furthermore, a great difference was found for the number of bee visits and seed yield among the onion lines analyzed. This study demonstrates that the combination
of chemical information and statistical analysis is able to differentiate onion male sterile lines from fertile lines, as well as among male sterile lines. Repellent compounds, such as dioxolanes, piperidines and
organosulfurs may contribute negatively to pollination, since the higher content of these analytes, the less bee visits and seed production yield. |
| format | Artículo |
| id | INTA1375 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA13752018-11-15T14:22:03Z Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production Soto Vargas, Verónica Carolina Maldonado, Irma Beatriz Jofre, Viviana Patricia Galmarini, Claudio Romulo Silva, Maria Fernanda Cebolla Allium Cepa Néctar Compuestos Ogánicos Volátiles Polinización Producción de Semillas Onions Oganic Vlatile Compounds Pollination Seed Production Gas Chromatography SPME GC–MS In this study, a simple and solvent-free method using headspace solid-phase microextraction (SPME) and gas chromatography–mass spectrometry (GC–MS) was developed for determination of the volatile compounds from fresh flowers and nectar of male sterile and fertile lines of Allium cepa L. The SPME parameters were studied; the optimum conditions of 85 μm carboxen/polydimethylsiloxane (CAR/PDMS), extraction temperature of 30 °C and extraction time of 30 min were obtained. The analytical method was applied to characterize different onion lines according to the chemical composition of the volatile fraction of nectar and flowers. On the other hand, it was determined which odor components contribute to the pollination of onions lines and relate the content of specific analytes with foraging behavior and seed production. More than 90 compounds were identified. The samples studied showed differences in the volatile profiles of flowers and nectar among the different lines. The results demonstrated that headspace SPME–GCMS is a simple, rapid and solvent-free method suitable for analysis of volatile compounds emitted from onion plants. Furthermore, a great difference was found for the number of bee visits and seed yield among the onion lines analyzed. This study demonstrates that the combination of chemical information and statistical analysis is able to differentiate onion male sterile lines from fertile lines, as well as among male sterile lines. Repellent compounds, such as dioxolanes, piperidines and organosulfurs may contribute negatively to pollination, since the higher content of these analytes, the less bee visits and seed production yield. EEA Mendoza Fil: Soto, Veronica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Maldonado, Irma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria El Colorado. Agencia de Extensión Rural Formosa; Argentina Fil: Jofre, Viviana Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencia Agrarias: Argentina Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina 2017-10-02T14:16:06Z 2017-10-02T14:16:06Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1375 http://www.sciencedirect.com/science/article/pii/S0026265X15000788 https://doi.org/10.1016/j.microc.2015.04.017 eng info:eu-repo/semantics/restrictedAccess application/pdf Microchemical journal 122 : 110-118. (September 2015) |
| spellingShingle | Cebolla Allium Cepa Néctar Compuestos Ogánicos Volátiles Polinización Producción de Semillas Onions Oganic Vlatile Compounds Pollination Seed Production Gas Chromatography SPME GC–MS Soto Vargas, Verónica Carolina Maldonado, Irma Beatriz Jofre, Viviana Patricia Galmarini, Claudio Romulo Silva, Maria Fernanda Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production |
| title | Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production |
| title_full | Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production |
| title_fullStr | Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production |
| title_full_unstemmed | Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production |
| title_short | Direct analysis of néctar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production |
| title_sort | direct analysis of nectar and floral volatile organic compounds in hybrid onions by hs spme gc ms relationship with pollination and seed production |
| topic | Cebolla Allium Cepa Néctar Compuestos Ogánicos Volátiles Polinización Producción de Semillas Onions Oganic Vlatile Compounds Pollination Seed Production Gas Chromatography SPME GC–MS |
| url | http://hdl.handle.net/20.500.12123/1375 http://www.sciencedirect.com/science/article/pii/S0026265X15000788 https://doi.org/10.1016/j.microc.2015.04.017 |
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