The Gln15Arg mutation in the transcriptional factor PALM1 produces multifoliate alfalfa
The CRISPR/Cas9 system has proven to be highly valuable for genome editing in several important crops, including the allogamous tetraploid cultivated alfalfa (Medicago sativa). However, the fact that most of the beneficial mutations relevant to alfalfa breeding programs are recessive mutations ident...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/13730 https://link.springer.com/article/10.1007/s11240-022-02429-8 https://doi.org/10.1007/s11240-022-02429-8 |
| _version_ | 1855485227019796480 |
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| author | Gomez, Maria Cristina Jozefkowicz, Cintia Mozzicafreddo, Matteo Odorizzi, Ariel Arolfo, Valeria Basigalup, Daniel Horacio Ayub, Nicolás Daniel Soto, Gabriela Cynthia |
| author_browse | Arolfo, Valeria Ayub, Nicolás Daniel Basigalup, Daniel Horacio Gomez, Maria Cristina Jozefkowicz, Cintia Mozzicafreddo, Matteo Odorizzi, Ariel Soto, Gabriela Cynthia |
| author_facet | Gomez, Maria Cristina Jozefkowicz, Cintia Mozzicafreddo, Matteo Odorizzi, Ariel Arolfo, Valeria Basigalup, Daniel Horacio Ayub, Nicolás Daniel Soto, Gabriela Cynthia |
| author_sort | Gomez, Maria Cristina |
| collection | INTA Digital |
| description | The CRISPR/Cas9 system has proven to be highly valuable for genome editing in several important crops, including the allogamous tetraploid cultivated alfalfa (Medicago sativa). However, the fact that most of the beneficial mutations relevant to alfalfa breeding programs are recessive mutations identified in the autogamous diploid species Medicago truncatula is a limitation. Naturally, the identification of dominant mutations can overcome this constraint and maximize the benefits of genome editing. In this article, we present the identification of a novel dominant mutation producing multifoliate leaves. Specifically, we showed that the mutation of the conserved glutamine 15 of the transcriptional factor PALM1 to arginine produces heptafoliate leaves in alfalfa, improving its digestibility and nutritional quality. Finally, we discuss the potential use of this mutation to rapidly generate transgenic-free legume forages with higher nutritional value via adenine base editors. |
| format | Artículo |
| id | INTA13730 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA137302022-12-28T09:53:39Z The Gln15Arg mutation in the transcriptional factor PALM1 produces multifoliate alfalfa Gomez, Maria Cristina Jozefkowicz, Cintia Mozzicafreddo, Matteo Odorizzi, Ariel Arolfo, Valeria Basigalup, Daniel Horacio Ayub, Nicolás Daniel Soto, Gabriela Cynthia Medicago sativa Mutación Factores de Transcripción Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Edición de Genes Mutation Transcription Factors CRISPR Gene Editing Alfalfa Lucerne The CRISPR/Cas9 system has proven to be highly valuable for genome editing in several important crops, including the allogamous tetraploid cultivated alfalfa (Medicago sativa). However, the fact that most of the beneficial mutations relevant to alfalfa breeding programs are recessive mutations identified in the autogamous diploid species Medicago truncatula is a limitation. Naturally, the identification of dominant mutations can overcome this constraint and maximize the benefits of genome editing. In this article, we present the identification of a novel dominant mutation producing multifoliate leaves. Specifically, we showed that the mutation of the conserved glutamine 15 of the transcriptional factor PALM1 to arginine produces heptafoliate leaves in alfalfa, improving its digestibility and nutritional quality. Finally, we discuss the potential use of this mutation to rapidly generate transgenic-free legume forages with higher nutritional value via adenine base editors. Instituto de Biotecnología Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Gomez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Jozefkowicz, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Mozzicafreddo, Matteo. Università Politecnica delle Marche. Department of Clinical and Molecular Sciences; Italia Fil: Odorizzi, A. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Arolfo, V. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Basigalup, D. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Soto, Gabriela Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2022-12-28T09:49:48Z 2022-12-28T09:49:48Z 2022-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13730 https://link.springer.com/article/10.1007/s11240-022-02429-8 1573-5044 https://doi.org/10.1007/s11240-022-02429-8 eng info:eu-repograntAgreement/INTA/2019-PE-E6-I115-001/2019-PE-E6-I115-001/AR./Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuario info:eu-repo/semantics/restrictedAccess application/pdf Springer Plant Cell, Tissue and Organ Culture (Published: 20 December 2022) |
| spellingShingle | Medicago sativa Mutación Factores de Transcripción Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Edición de Genes Mutation Transcription Factors CRISPR Gene Editing Alfalfa Lucerne Gomez, Maria Cristina Jozefkowicz, Cintia Mozzicafreddo, Matteo Odorizzi, Ariel Arolfo, Valeria Basigalup, Daniel Horacio Ayub, Nicolás Daniel Soto, Gabriela Cynthia The Gln15Arg mutation in the transcriptional factor PALM1 produces multifoliate alfalfa |
| title | The Gln15Arg mutation in the transcriptional factor PALM1 produces multifoliate alfalfa |
| title_full | The Gln15Arg mutation in the transcriptional factor PALM1 produces multifoliate alfalfa |
| title_fullStr | The Gln15Arg mutation in the transcriptional factor PALM1 produces multifoliate alfalfa |
| title_full_unstemmed | The Gln15Arg mutation in the transcriptional factor PALM1 produces multifoliate alfalfa |
| title_short | The Gln15Arg mutation in the transcriptional factor PALM1 produces multifoliate alfalfa |
| title_sort | gln15arg mutation in the transcriptional factor palm1 produces multifoliate alfalfa |
| topic | Medicago sativa Mutación Factores de Transcripción Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Edición de Genes Mutation Transcription Factors CRISPR Gene Editing Alfalfa Lucerne |
| url | http://hdl.handle.net/20.500.12123/13730 https://link.springer.com/article/10.1007/s11240-022-02429-8 https://doi.org/10.1007/s11240-022-02429-8 |
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