Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens
Wood properties and agronomic traits associated with fast growth and frost tolerance make Eucalyptus nitens a valuable forest alternative. However, the rapid age-related decline in the adventitious root (AR) formation (herein, meaning induction, initiation, and expression stages) limits its propagat...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/13704 https://www.mdpi.com/2223-7747/11/23/3301 https://doi.org/10.3390/plants11233301 |
| _version_ | 1855485222032769024 |
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| author | Ayala, Paula Gabriela Acevedo, Raúl Maximiliano Luna, Claudia Verónica Rivarola, Maximo Lisandro Acuña, Cintia Vanesa Marcucci Poltri, Susana Noemi González, Ana M. Sansberro, Pedro Alfonso |
| author_browse | Acevedo, Raúl Maximiliano Acuña, Cintia Vanesa Ayala, Paula Gabriela González, Ana M. Luna, Claudia Verónica Marcucci Poltri, Susana Noemi Rivarola, Maximo Lisandro Sansberro, Pedro Alfonso |
| author_facet | Ayala, Paula Gabriela Acevedo, Raúl Maximiliano Luna, Claudia Verónica Rivarola, Maximo Lisandro Acuña, Cintia Vanesa Marcucci Poltri, Susana Noemi González, Ana M. Sansberro, Pedro Alfonso |
| author_sort | Ayala, Paula Gabriela |
| collection | INTA Digital |
| description | Wood properties and agronomic traits associated with fast growth and frost tolerance make Eucalyptus nitens a valuable forest alternative. However, the rapid age-related decline in the adventitious root (AR) formation (herein, meaning induction, initiation, and expression stages) limits its propagation. We analyzed transcriptomic profile variation in leaves and stem bases during AR induction of microcuttings to elucidate the molecular mechanisms involved in AR formation. In addition, we quantified expressions of candidate genes associated with recalcitrance. We delimited the ontogenic phases of root formation using histological techniques and Scarecrow and Short-Root expression quantification for RNA sequencing sample collection. We quantified the gene expressions associated with root meristem formation, auxin biosynthesis, perception, signaling, conjugation, and cytokinin signaling in shoots harvested from 2- to 36-month-old plants. After IBA treatment, 702 transcripts changed their expressions. Several were involved in hormone homeostasis and the signaling pathways that determine cell dedifferentiation, leading to root meristem formation. In part, the age-related decline in the rooting capacity is attributable to the increase in the ARR1 gene expression, which negatively affects auxin homeostasis. The analysis of the transcriptomic variation in the leaves and rooting zones provided profuse information: (1) To elucidate the auxin metabolism; (2) to understand the hormonal and signaling processes involved; (3) to collect data associated with their recalcitrance. |
| format | Artículo |
| id | INTA13704 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA137042022-12-26T13:39:20Z Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens Ayala, Paula Gabriela Acevedo, Raúl Maximiliano Luna, Claudia Verónica Rivarola, Maximo Lisandro Acuña, Cintia Vanesa Marcucci Poltri, Susana Noemi González, Ana M. Sansberro, Pedro Alfonso Eucalyptus nitens Raíces Adventicias Transcriptomas Auxinas Adventitious Roots Transcriptome Auxins In vitro Wood properties and agronomic traits associated with fast growth and frost tolerance make Eucalyptus nitens a valuable forest alternative. However, the rapid age-related decline in the adventitious root (AR) formation (herein, meaning induction, initiation, and expression stages) limits its propagation. We analyzed transcriptomic profile variation in leaves and stem bases during AR induction of microcuttings to elucidate the molecular mechanisms involved in AR formation. In addition, we quantified expressions of candidate genes associated with recalcitrance. We delimited the ontogenic phases of root formation using histological techniques and Scarecrow and Short-Root expression quantification for RNA sequencing sample collection. We quantified the gene expressions associated with root meristem formation, auxin biosynthesis, perception, signaling, conjugation, and cytokinin signaling in shoots harvested from 2- to 36-month-old plants. After IBA treatment, 702 transcripts changed their expressions. Several were involved in hormone homeostasis and the signaling pathways that determine cell dedifferentiation, leading to root meristem formation. In part, the age-related decline in the rooting capacity is attributable to the increase in the ARR1 gene expression, which negatively affects auxin homeostasis. The analysis of the transcriptomic variation in the leaves and rooting zones provided profuse information: (1) To elucidate the auxin metabolism; (2) to understand the hormonal and signaling processes involved; (3) to collect data associated with their recalcitrance. Instituto de Biotecnología Fil: Ayala, Paula Gabriela. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina Fil: Ayala, Paula Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayala, Paula Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Acevedo, Raúl M. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina Fil: Acevedo, Raúl M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Luna, Claudia Verónica. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina Fil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Marcucci Poltri, Susana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: González, Ana M. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina Fil: González, Ana M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sansberro, Pedro Alfonso. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2022-12-26T13:34:28Z 2022-12-26T13:34:28Z 2022-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13704 https://www.mdpi.com/2223-7747/11/23/3301 2223-7747 https://doi.org/10.3390/plants11233301 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Plants 11 (23) : 3301 (Noviembre 2022) |
| spellingShingle | Eucalyptus nitens Raíces Adventicias Transcriptomas Auxinas Adventitious Roots Transcriptome Auxins In vitro Ayala, Paula Gabriela Acevedo, Raúl Maximiliano Luna, Claudia Verónica Rivarola, Maximo Lisandro Acuña, Cintia Vanesa Marcucci Poltri, Susana Noemi González, Ana M. Sansberro, Pedro Alfonso Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens |
| title | Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens |
| title_full | Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens |
| title_fullStr | Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens |
| title_full_unstemmed | Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens |
| title_short | Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens |
| title_sort | transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens |
| topic | Eucalyptus nitens Raíces Adventicias Transcriptomas Auxinas Adventitious Roots Transcriptome Auxins In vitro |
| url | http://hdl.handle.net/20.500.12123/13704 https://www.mdpi.com/2223-7747/11/23/3301 https://doi.org/10.3390/plants11233301 |
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