Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice
Profilins are actin-binding proteins that regulate the polymerization of actin filaments. In apicomplexan parasites, they are essential for invasion. Profilins also trigger the immune response of the host by activating TLRs on dendritic cells (DCs), inducing the production of pro-inflammatory cytoki...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1370 http://www.sciencedirect.com/science/article/pii/S0014489415300552 https://doi.org/10.1016/j.exppara.2015.10.008 |
| _version_ | 1855034792592015360 |
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| author | Mansilla, Florencia Celeste Quintana, Maria Eugenia Langellotti, Cecilia Ana Wilda, Maximiliano Martinez, Andrea Fonzo, Adriana Moore, Prando Dadin Cardoso, Nancy Capozzo, Alejandra |
| author_browse | Capozzo, Alejandra Cardoso, Nancy Fonzo, Adriana Langellotti, Cecilia Ana Mansilla, Florencia Celeste Martinez, Andrea Moore, Prando Dadin Quintana, Maria Eugenia Wilda, Maximiliano |
| author_facet | Mansilla, Florencia Celeste Quintana, Maria Eugenia Langellotti, Cecilia Ana Wilda, Maximiliano Martinez, Andrea Fonzo, Adriana Moore, Prando Dadin Cardoso, Nancy Capozzo, Alejandra |
| author_sort | Mansilla, Florencia Celeste |
| collection | INTA Digital |
| description | Profilins are actin-binding proteins that regulate the polymerization of actin filaments. In apicomplexan parasites, they are essential for invasion. Profilins also trigger the immune response of the host by activating TLRs on dendritic cells (DCs), inducing the production of pro-inflammatory cytokines. In this study we characterized for the first time the immune response and protection elicited by a vaccine based on Neospora caninum profilin in mice. Groups of eight BALB/c mice received either two doses of a recombinant N. caninum profilin expressed in Escherichia coli. (rNcPRO) or PBS, both formulated with an aqueous soy-based adjuvant enriched in TLR-agonists. Specific anti-profilin antibodies were detected in rNcPRO-vaccinated animals, mainly IgM and IgG3, which were consumed after infection. Splenocytes from rNcPRO-immunized animals proliferated after an in vitro stimulation with rNcPRO before and after challenge. An impairment of the cellular response was observed in NcPRO vaccinated and infected mice following an in vitro stimulation with native antigens of N. caninum, related to an increase in the percentage of CD4þCD25þFoxP3þ. Two out of five rNcPRO-vaccinated challenged mice were protected; they were negative for parasite DNA in the brain and showed no histopathological lesions, which were found in all PBS-vaccinated animals. As a whole, our results provide evidence of a regulatory response elicited by immunization with rNcPRO, and suggest a role of profilin in the modulation and/or evasion of immune responses against N. caninum. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1370 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA13702019-03-22T14:34:18Z Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice Mansilla, Florencia Celeste Quintana, Maria Eugenia Langellotti, Cecilia Ana Wilda, Maximiliano Martinez, Andrea Fonzo, Adriana Moore, Prando Dadin Cardoso, Nancy Capozzo, Alejandra Enfermedades de los Animales Neospora Caninum Inmunización Linfocitos-t Ratón Animal Diseases Immunization T-lymphocytes Mice Profilins are actin-binding proteins that regulate the polymerization of actin filaments. In apicomplexan parasites, they are essential for invasion. Profilins also trigger the immune response of the host by activating TLRs on dendritic cells (DCs), inducing the production of pro-inflammatory cytokines. In this study we characterized for the first time the immune response and protection elicited by a vaccine based on Neospora caninum profilin in mice. Groups of eight BALB/c mice received either two doses of a recombinant N. caninum profilin expressed in Escherichia coli. (rNcPRO) or PBS, both formulated with an aqueous soy-based adjuvant enriched in TLR-agonists. Specific anti-profilin antibodies were detected in rNcPRO-vaccinated animals, mainly IgM and IgG3, which were consumed after infection. Splenocytes from rNcPRO-immunized animals proliferated after an in vitro stimulation with rNcPRO before and after challenge. An impairment of the cellular response was observed in NcPRO vaccinated and infected mice following an in vitro stimulation with native antigens of N. caninum, related to an increase in the percentage of CD4þCD25þFoxP3þ. Two out of five rNcPRO-vaccinated challenged mice were protected; they were negative for parasite DNA in the brain and showed no histopathological lesions, which were found in all PBS-vaccinated animals. As a whole, our results provide evidence of a regulatory response elicited by immunization with rNcPRO, and suggest a role of profilin in the modulation and/or evasion of immune responses against N. caninum. Inst.de Virología Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Quintana, Maria Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Wilda, Maximiliano. Tecnovax S.A,; Argentina Fil: Martinez, Andrea. Instituto de Ciencia y Tecnología Dr. Céesar Milstein; Argentina Fil: Fonzo, Adriana. Instituto de Ciencia y Tecnología Dr. Céesar Milstein; Argentina Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Cardoso, Nancy. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina 2017-10-02T12:25:42Z 2017-10-02T12:25:42Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1370 http://www.sciencedirect.com/science/article/pii/S0014489415300552 0014-4894 (Print) 1090-2449 (Online) https://doi.org/10.1016/j.exppara.2015.10.008 eng info:eu-repo/semantics/restrictedAccess application/pdf Experimental parasitology 160 : 1-10. (January 2016) |
| spellingShingle | Enfermedades de los Animales Neospora Caninum Inmunización Linfocitos-t Ratón Animal Diseases Immunization T-lymphocytes Mice Mansilla, Florencia Celeste Quintana, Maria Eugenia Langellotti, Cecilia Ana Wilda, Maximiliano Martinez, Andrea Fonzo, Adriana Moore, Prando Dadin Cardoso, Nancy Capozzo, Alejandra Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice |
| title | Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice |
| title_full | Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice |
| title_fullStr | Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice |
| title_full_unstemmed | Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice |
| title_short | Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice |
| title_sort | immunization with neospora caninum profilin induces limited protection and a regulatory t cell response in mice |
| topic | Enfermedades de los Animales Neospora Caninum Inmunización Linfocitos-t Ratón Animal Diseases Immunization T-lymphocytes Mice |
| url | http://hdl.handle.net/20.500.12123/1370 http://www.sciencedirect.com/science/article/pii/S0014489415300552 https://doi.org/10.1016/j.exppara.2015.10.008 |
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