Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus
The aim of this study was to evaluate the vectorial competence of Rhipicephalus microplus to transmit Anaplasma marginale transovarially, by analyzing the results of three different but complementary assays. First, larvae of R. microplus were fed on a calf infected with the isolate S1P of A. margina...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/13684 https://www.sciencedirect.com/science/article/abs/pii/S0304401722001625 https://doi.org/10.1016/j.vetpar.2022.109808 |
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| author | Mazzucco Panizza, Matilde Nahimé Cutulle, Christian Primo, María Evangelina Morel, Nicolas Sebastian, Patrick Nava, Santiago |
| author_browse | Cutulle, Christian Mazzucco Panizza, Matilde Nahimé Morel, Nicolas Nava, Santiago Primo, María Evangelina Sebastian, Patrick |
| author_facet | Mazzucco Panizza, Matilde Nahimé Cutulle, Christian Primo, María Evangelina Morel, Nicolas Sebastian, Patrick Nava, Santiago |
| author_sort | Mazzucco Panizza, Matilde Nahimé |
| collection | INTA Digital |
| description | The aim of this study was to evaluate the vectorial competence of Rhipicephalus microplus to transmit Anaplasma marginale transovarially, by analyzing the results of three different but complementary assays. First, larvae of R. microplus were fed on a calf infected with the isolate S1P of A. marginale. The engorged females obtained were analyzed by PCR and incubated for oviposition. After hatching, larvae were analyzed by PCR and fed on susceptible splenectomized cattle. Although A. marginale was detected in the females, no A. marginale DNA was amplified from the larvae and transmission of A. marginale to cattle was not recorded. In the second experiment, R. microplus larvae were fed on cattle naturally infected with field isolates of A. marginale and experimentally infected with the isolate S1P of A. marginale. After detachment, engorged females were incubated for oviposition. The offspring were analyzed by PCR, with negative results. Finally, free-living larvae of R. microplus collected from pasture on farms with cattle infected with A. marginale were analyzed by PCR for Anaplasma infection. All samples analyzed were negative for A. marginale. The results of this work indicate that transovarial transmission of A. marginale by R. microplus is unlikely to occur. |
| format | info:ar-repo/semantics/artículo |
| id | INTA13684 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA136842022-12-22T11:38:02Z Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus Mazzucco Panizza, Matilde Nahimé Cutulle, Christian Primo, María Evangelina Morel, Nicolas Sebastian, Patrick Nava, Santiago Anaplasma marginale Rhipicephalus Anaplasmosis Ganado Bovino Cattle Transovarial Transmission Transmisión Transovárica Rhipicephalus microplus The aim of this study was to evaluate the vectorial competence of Rhipicephalus microplus to transmit Anaplasma marginale transovarially, by analyzing the results of three different but complementary assays. First, larvae of R. microplus were fed on a calf infected with the isolate S1P of A. marginale. The engorged females obtained were analyzed by PCR and incubated for oviposition. After hatching, larvae were analyzed by PCR and fed on susceptible splenectomized cattle. Although A. marginale was detected in the females, no A. marginale DNA was amplified from the larvae and transmission of A. marginale to cattle was not recorded. In the second experiment, R. microplus larvae were fed on cattle naturally infected with field isolates of A. marginale and experimentally infected with the isolate S1P of A. marginale. After detachment, engorged females were incubated for oviposition. The offspring were analyzed by PCR, with negative results. Finally, free-living larvae of R. microplus collected from pasture on farms with cattle infected with A. marginale were analyzed by PCR for Anaplasma infection. All samples analyzed were negative for A. marginale. The results of this work indicate that transovarial transmission of A. marginale by R. microplus is unlikely to occur. EEA Rafaela Fil: Mazzucco Panizza, Matilde Nahimé. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Mazzucco Panizza, Matilde Nahimé. Instituto Nacional de Tecnología Agropecuaria. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Cutulle, Christian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Buenos Aires, Argentina Fil: Primo, María Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Primo, María Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina 2022-12-22T11:31:09Z 2022-12-22T11:31:09Z 2022-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13684 https://www.sciencedirect.com/science/article/abs/pii/S0304401722001625 0304-4017 (print) 1873-2550 (online) https://doi.org/10.1016/j.vetpar.2022.109808 eng info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001/2019-PE-E5-I109-001/AR./Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Parasitology 311: 109808 (November 2022) |
| spellingShingle | Anaplasma marginale Rhipicephalus Anaplasmosis Ganado Bovino Cattle Transovarial Transmission Transmisión Transovárica Rhipicephalus microplus Mazzucco Panizza, Matilde Nahimé Cutulle, Christian Primo, María Evangelina Morel, Nicolas Sebastian, Patrick Nava, Santiago Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus |
| title | Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus |
| title_full | Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus |
| title_fullStr | Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus |
| title_full_unstemmed | Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus |
| title_short | Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus |
| title_sort | assays to evaluate the transovarial transmission of anaplasma marginale by rhipicephalus microplus |
| topic | Anaplasma marginale Rhipicephalus Anaplasmosis Ganado Bovino Cattle Transovarial Transmission Transmisión Transovárica Rhipicephalus microplus |
| url | http://hdl.handle.net/20.500.12123/13684 https://www.sciencedirect.com/science/article/abs/pii/S0304401722001625 https://doi.org/10.1016/j.vetpar.2022.109808 |
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