Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
The anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange r...
| Autores principales: | , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Academia Brasileira de Ciencias
2022
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/13679 https://www.scielo.br/j/aabc/a/c4KdzQ6PfcMs7SzyxHxHdMm/?lang=en https://doi.org/10.1590/0001-3765202220210672 |
| _version_ | 1855036979671990272 |
|---|---|
| author | Arizio, Carla Marcela Costa Tártara, Sabrina María Zunino, Ignacio Manifesto, Maria Marcela |
| author_browse | Arizio, Carla Marcela Costa Tártara, Sabrina María Manifesto, Maria Marcela Zunino, Ignacio |
| author_facet | Arizio, Carla Marcela Costa Tártara, Sabrina María Zunino, Ignacio Manifesto, Maria Marcela |
| author_sort | Arizio, Carla Marcela |
| collection | INTA Digital |
| description | The anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and segregation analysis of those genes in sweet potato hexaploid species are still unknown. Based on an “exon-primed intron crossing” (EPIC) approach, fourteen pairs of primers were designed, on fi ve structural anthocyanin genes as candidates. The strategy exploits the Intron Length Polymorphism (ILP) from Candidate Genes (CG), resulting in 93% of successful markers giving scorable and reproducible alleles. The results allowed to defi ne partial structure and sequence of the introns and exons from the selected CG, and to determine patterns of sequence variation. The evaluation of marker dosage and allelic segregations in an Ipomoea batatas (L.) Lam mapping population identifi ed several alleles for linkage analysis. The study validated the utility of ILP-CG markers for genetic diversity and conservation applicability and a successful amplifi cation gradient across wild Ipomoea species validated their transferability |
| format | info:ar-repo/semantics/artículo |
| id | INTA13679 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Academia Brasileira de Ciencias |
| publisherStr | Academia Brasileira de Ciencias |
| record_format | dspace |
| spelling | INTA136792022-12-22T10:35:36Z Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species Arizio, Carla Marcela Costa Tártara, Sabrina María Zunino, Ignacio Manifesto, Maria Marcela Anthocyanins Candidate Genes Genetic Diversity (as resource) Genetic Markers Antocianinas Genes Candidatos Diversidad Genética (como recurso) Ipomoea Marcadores Genéticos ILP-CG Markers Wild Ipomoeas Marcadores ILP-CG Ipomoeas Salvajes The anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and segregation analysis of those genes in sweet potato hexaploid species are still unknown. Based on an “exon-primed intron crossing” (EPIC) approach, fourteen pairs of primers were designed, on fi ve structural anthocyanin genes as candidates. The strategy exploits the Intron Length Polymorphism (ILP) from Candidate Genes (CG), resulting in 93% of successful markers giving scorable and reproducible alleles. The results allowed to defi ne partial structure and sequence of the introns and exons from the selected CG, and to determine patterns of sequence variation. The evaluation of marker dosage and allelic segregations in an Ipomoea batatas (L.) Lam mapping population identifi ed several alleles for linkage analysis. The study validated the utility of ILP-CG markers for genetic diversity and conservation applicability and a successful amplifi cation gradient across wild Ipomoea species validated their transferability Fil: Arizio, Carla Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Costa Tártara, Sabrina María. Universidad Nacional de Luján; Argentina Fil: Zunino, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural Mercedes; Argentina Fil: Manifesto, Maria Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina 2022-12-22T10:22:13Z 2022-12-22T10:22:13Z 2022 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13679 https://www.scielo.br/j/aabc/a/c4KdzQ6PfcMs7SzyxHxHdMm/?lang=en 0001-3765 1678-2690 https://doi.org/10.1590/0001-3765202220210672 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Academia Brasileira de Ciencias Anais da Academia Brasileira de Ciencias 94 (3) : e20210672 (2022) |
| spellingShingle | Anthocyanins Candidate Genes Genetic Diversity (as resource) Genetic Markers Antocianinas Genes Candidatos Diversidad Genética (como recurso) Ipomoea Marcadores Genéticos ILP-CG Markers Wild Ipomoeas Marcadores ILP-CG Ipomoeas Salvajes Arizio, Carla Marcela Costa Tártara, Sabrina María Zunino, Ignacio Manifesto, Maria Marcela Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species |
| title | Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species |
| title_full | Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species |
| title_fullStr | Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species |
| title_full_unstemmed | Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species |
| title_short | Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species |
| title_sort | development of anthocyanin markers gene mapping genomic analysis and genetic diversity studies in ipomoea species |
| topic | Anthocyanins Candidate Genes Genetic Diversity (as resource) Genetic Markers Antocianinas Genes Candidatos Diversidad Genética (como recurso) Ipomoea Marcadores Genéticos ILP-CG Markers Wild Ipomoeas Marcadores ILP-CG Ipomoeas Salvajes |
| url | http://hdl.handle.net/20.500.12123/13679 https://www.scielo.br/j/aabc/a/c4KdzQ6PfcMs7SzyxHxHdMm/?lang=en https://doi.org/10.1590/0001-3765202220210672 |
| work_keys_str_mv | AT ariziocarlamarcela developmentofanthocyaninmarkersgenemappinggenomicanalysisandgeneticdiversitystudiesinipomoeaspecies AT costatartarasabrinamaria developmentofanthocyaninmarkersgenemappinggenomicanalysisandgeneticdiversitystudiesinipomoeaspecies AT zuninoignacio developmentofanthocyaninmarkersgenemappinggenomicanalysisandgeneticdiversitystudiesinipomoeaspecies AT manifestomariamarcela developmentofanthocyaninmarkersgenemappinggenomicanalysisandgeneticdiversitystudiesinipomoeaspecies |