Fusion of foreign T-cell epitopes and addition of TLR agonists enhance immunity against Neospora caninum profilin in cattle
We demonstrated recently that immunization with recombinant Neospora caninum profilin (rNcPRO) induces limited protection and a regulatory T-cell response in mice. The aim of this study was to evaluate the immune response elicited by rNcPRO in cattle and assess a strategy to enhance its immunogeni...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1363 http://onlinelibrary.wiley.com/doi/10.1111/pim.12354/abstract;jsessionid=F7B7B93C207D63E18F13BCDDABE00D30.f03t03 |
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| author | Mansilla, Florencia Celeste Quintana, Maria Eugenia Cardoso, Nancy Capozzo, Alejandra |
| author_browse | Capozzo, Alejandra Cardoso, Nancy Mansilla, Florencia Celeste Quintana, Maria Eugenia |
| author_facet | Mansilla, Florencia Celeste Quintana, Maria Eugenia Cardoso, Nancy Capozzo, Alejandra |
| author_sort | Mansilla, Florencia Celeste |
| collection | INTA Digital |
| description | We demonstrated recently that immunization with recombinant Neospora caninum profilin (rNcPRO) induces limited protection and a regulatory T-cell
response in mice.
The aim of this study was to evaluate the immune response elicited by rNcPRO in cattle and assess a strategy to enhance its immunogenicity, combining the addition of T-cell epitopes and immune modulators. We developed a chimeric recombinant profilin fused to functional T-cell epitopes present in the N terminal sequence of vesicular stomatitis virus (VSV) glycoprotein G (rNcPRO/G). Groups of three cattle were immunized with two doses (2 weeks apart) of rNcPRO or rNcPRO/G formulated with alum hydroxide or a nanoparticulated soya-based adjuvant enriched with Toll-like receptor (TLR) 2 and TLR9 agonists, aimed to tackle the MyD88 pathway (AVECplus). rNcPRO induced only a primary immune response (IgM mediated), while antibodies in rNcPRO/G-vaccinated
animals switched to IgG1 after the booster. The vaccine formulated with
rNcPRO/G and AVECplus improved the production of systemic IFN-γ and induced
long-term recall B-cell responses. Overall, our study provides data supporting the use of T-cell epitopes from VSV glycoprotein G and TLR agonists to enhance and modulate immunity to peptide antigens in bovines, particularly when using small proteins from parasites for which immune responses are usually feeble. |
| format | Artículo |
| id | INTA1363 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA13632019-03-22T14:33:15Z Fusion of foreign T-cell epitopes and addition of TLR agonists enhance immunity against Neospora caninum profilin in cattle Mansilla, Florencia Celeste Quintana, Maria Eugenia Cardoso, Nancy Capozzo, Alejandra Genetics Cattle T-lymphocytes Immunity Genética Ganado Bovino Neospora Caninum Linfocitos-t Inmunidad We demonstrated recently that immunization with recombinant Neospora caninum profilin (rNcPRO) induces limited protection and a regulatory T-cell response in mice. The aim of this study was to evaluate the immune response elicited by rNcPRO in cattle and assess a strategy to enhance its immunogenicity, combining the addition of T-cell epitopes and immune modulators. We developed a chimeric recombinant profilin fused to functional T-cell epitopes present in the N terminal sequence of vesicular stomatitis virus (VSV) glycoprotein G (rNcPRO/G). Groups of three cattle were immunized with two doses (2 weeks apart) of rNcPRO or rNcPRO/G formulated with alum hydroxide or a nanoparticulated soya-based adjuvant enriched with Toll-like receptor (TLR) 2 and TLR9 agonists, aimed to tackle the MyD88 pathway (AVECplus). rNcPRO induced only a primary immune response (IgM mediated), while antibodies in rNcPRO/G-vaccinated animals switched to IgG1 after the booster. The vaccine formulated with rNcPRO/G and AVECplus improved the production of systemic IFN-γ and induced long-term recall B-cell responses. Overall, our study provides data supporting the use of T-cell epitopes from VSV glycoprotein G and TLR agonists to enhance and modulate immunity to peptide antigens in bovines, particularly when using small proteins from parasites for which immune responses are usually feeble. Inst.de Virología Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Quintana, Maria Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cardoso, Nancy. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-09-29T15:19:32Z 2017-09-29T15:19:32Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1363 http://onlinelibrary.wiley.com/doi/10.1111/pim.12354/abstract;jsessionid=F7B7B93C207D63E18F13BCDDABE00D30.f03t03 0141-9838 (Print) 1365-3024 (Online) DOI: 10.1111/pim.12354 eng info:eu-repo/semantics/restrictedAccess application/pdf Parasite immunology 38 (11) : 663–669. (November 2016) |
| spellingShingle | Genetics Cattle T-lymphocytes Immunity Genética Ganado Bovino Neospora Caninum Linfocitos-t Inmunidad Mansilla, Florencia Celeste Quintana, Maria Eugenia Cardoso, Nancy Capozzo, Alejandra Fusion of foreign T-cell epitopes and addition of TLR agonists enhance immunity against Neospora caninum profilin in cattle |
| title | Fusion of foreign T-cell epitopes and addition of TLR agonists enhance immunity against Neospora caninum profilin in cattle |
| title_full | Fusion of foreign T-cell epitopes and addition of TLR agonists enhance immunity against Neospora caninum profilin in cattle |
| title_fullStr | Fusion of foreign T-cell epitopes and addition of TLR agonists enhance immunity against Neospora caninum profilin in cattle |
| title_full_unstemmed | Fusion of foreign T-cell epitopes and addition of TLR agonists enhance immunity against Neospora caninum profilin in cattle |
| title_short | Fusion of foreign T-cell epitopes and addition of TLR agonists enhance immunity against Neospora caninum profilin in cattle |
| title_sort | fusion of foreign t cell epitopes and addition of tlr agonists enhance immunity against neospora caninum profilin in cattle |
| topic | Genetics Cattle T-lymphocytes Immunity Genética Ganado Bovino Neospora Caninum Linfocitos-t Inmunidad |
| url | http://hdl.handle.net/20.500.12123/1363 http://onlinelibrary.wiley.com/doi/10.1111/pim.12354/abstract;jsessionid=F7B7B93C207D63E18F13BCDDABE00D30.f03t03 |
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