Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue
Bovine alphaherpesviruses (BoHV) 1 and 5 share many genetic and structural characteristics; however, they differ in their ability to cause encephalitis. Previous research suggests that this difference might be caused by a differential modulation of apoptosis. In this study, we analyzed the mRNA expr...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/13612 https://www.sciencedirect.com/science/article/pii/S0147957122001631 https://doi.org/10.1016/j.cimid.2022.101906 |
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| author | Martínez Cuesta, Lucía Nieto Farías, María Victoria Romeo, Florencia Verna, Andrea Elizabeth Pérez, Sandra |
| author_browse | Martínez Cuesta, Lucía Nieto Farías, María Victoria Pérez, Sandra Romeo, Florencia Verna, Andrea Elizabeth |
| author_facet | Martínez Cuesta, Lucía Nieto Farías, María Victoria Romeo, Florencia Verna, Andrea Elizabeth Pérez, Sandra |
| author_sort | Martínez Cuesta, Lucía |
| collection | INTA Digital |
| description | Bovine alphaherpesviruses (BoHV) 1 and 5 share many genetic and structural characteristics; however, they differ in their ability to cause encephalitis. Previous research suggests that this difference might be caused by a differential modulation of apoptosis. In this study, we analyzed the mRNA expression of Bax, Bcl-2, Fas and caspases 3 and 8 in neural tissue sections of BoHV-1 and BoHV-5 experimentally-infected cattle. Overall, Fas and caspase 3 mRNA was up-regulated during BoHV-5 acute infection, latency, and reactivation. Conversely, caspase 3 mRNA levels increased only in the olfactory cortex during BoHV-1 acute infection, and it was down-regulated during reactivation, while Fas was only up-regulated during BoHV-1 acute infection and latency. Moreover, Bax/Bcl-2 ratio was lower than 1 during BoHV-1 acute infection except in the trigeminal ganglion, whereas many brain regions exhibit a ratio higher than 1 during BoHV-5 acute infection and reactivation. In summary, our findings suggest that during acute infection and reactivation, BoHV-5 induces a pro-apoptotic condition that could partially justify its increased ability to cause neurological damage. |
| format | info:ar-repo/semantics/artículo |
| id | INTA13612 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA136122022-12-14T15:43:03Z Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue Martínez Cuesta, Lucía Nieto Farías, María Victoria Romeo, Florencia Verna, Andrea Elizabeth Pérez, Sandra Apoptosis Bovine Herpesvirus Neuropathy Cattle Herpes Virus Bovino Neuropatía Ganado Bovino Bovine alphaherpesviruses (BoHV) 1 and 5 share many genetic and structural characteristics; however, they differ in their ability to cause encephalitis. Previous research suggests that this difference might be caused by a differential modulation of apoptosis. In this study, we analyzed the mRNA expression of Bax, Bcl-2, Fas and caspases 3 and 8 in neural tissue sections of BoHV-1 and BoHV-5 experimentally-infected cattle. Overall, Fas and caspase 3 mRNA was up-regulated during BoHV-5 acute infection, latency, and reactivation. Conversely, caspase 3 mRNA levels increased only in the olfactory cortex during BoHV-1 acute infection, and it was down-regulated during reactivation, while Fas was only up-regulated during BoHV-1 acute infection and latency. Moreover, Bax/Bcl-2 ratio was lower than 1 during BoHV-1 acute infection except in the trigeminal ganglion, whereas many brain regions exhibit a ratio higher than 1 during BoHV-5 acute infection and reactivation. In summary, our findings suggest that during acute infection and reactivation, BoHV-5 induces a pro-apoptotic condition that could partially justify its increased ability to cause neurological damage. EEA Balcarce Fil: Martínez Cuesta, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Nieto Farías, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Romeo, Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Pérez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. 2022-12-14T15:39:45Z 2022-12-14T15:39:45Z 2022-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13612 https://www.sciencedirect.com/science/article/pii/S0147957122001631 0147-9571 https://doi.org/10.1016/j.cimid.2022.101906 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Comparative Immunology, Microbiology and Infectious Diseases 90–91: 101906 (November–December 2022) |
| spellingShingle | Apoptosis Bovine Herpesvirus Neuropathy Cattle Herpes Virus Bovino Neuropatía Ganado Bovino Martínez Cuesta, Lucía Nieto Farías, María Victoria Romeo, Florencia Verna, Andrea Elizabeth Pérez, Sandra Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue |
| title | Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue |
| title_full | Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue |
| title_fullStr | Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue |
| title_full_unstemmed | Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue |
| title_short | Expression of apoptosis-related genes at different stages of BoHV-1 and 5 infection of bovine neural tissue |
| title_sort | expression of apoptosis related genes at different stages of bohv 1 and 5 infection of bovine neural tissue |
| topic | Apoptosis Bovine Herpesvirus Neuropathy Cattle Herpes Virus Bovino Neuropatía Ganado Bovino |
| url | http://hdl.handle.net/20.500.12123/13612 https://www.sciencedirect.com/science/article/pii/S0147957122001631 https://doi.org/10.1016/j.cimid.2022.101906 |
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