Somatic embryogenesis from in vitro anther culture of apomictic buffel grass genotypes and analysis of regenerated plants using flow cytometry
Buffel grass is a fodder grass that reproduces mainly via apomixis. Using in vitro tissue culture in apomictic species provides alternatives that can be applied in a breeding program. The aims of this work were to establish a protocol for in vitro generation of apomictic buffel grass genotypes using...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1353 https://link.springer.com/article/10.1007/s11240-014-0441-4 https://doi.org/10.1007/s11240-014-0441-4 |
| _version_ | 1855034789953798144 |
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| author | Carloni, Edgardo José Ribotta, Andrea Noemi Lopez Colomba, Eliana Griffa, Sabrina Mariana Quiroga, Mariana Paola Tommasino, Exequiel Arturo Grunberg, Karina |
| author_browse | Carloni, Edgardo José Griffa, Sabrina Mariana Grunberg, Karina Lopez Colomba, Eliana Quiroga, Mariana Paola Ribotta, Andrea Noemi Tommasino, Exequiel Arturo |
| author_facet | Carloni, Edgardo José Ribotta, Andrea Noemi Lopez Colomba, Eliana Griffa, Sabrina Mariana Quiroga, Mariana Paola Tommasino, Exequiel Arturo Grunberg, Karina |
| author_sort | Carloni, Edgardo José |
| collection | INTA Digital |
| description | Buffel grass is a fodder grass that reproduces mainly via apomixis. Using in vitro tissue culture in apomictic species provides alternatives that can be applied in a breeding program. The aims of this work were to establish a protocol for in vitro generation of apomictic buffel grass genotypes using anthers as explants and to evaluate the genetic stability of regenerated plants via flow cytometry (FCM). Three genotypes were able to induce embryogenic calli in Murashige and Skoog (MS) medium supplemented with 6 mg/l of 2,4-dichlorophenoxyacetic acid. Seedling regeneration occurred in a MS medium supplemented with 0.5 mg/l napthaleneacetic acid + 1 mg/l 6-benzylaminopurin. Seedlings were derived from somatic embryos and the morphogenic process was induced using the somatic tissue of the stamens. Induction and regeneration efficiency depended on the genotype and was affected by date of tiller collection, different pretreatments or the interaction of these variables. FCM analyses in in vitro regenerated plants showed genetic instability in their nuclear DNA content. Plants with lower nuclear DNA content may indicate DNA aneuploids (8.6 %), whereas plants that had twice the value of nuclear DNA content (4.7 %) suggest in vitro polyploidization. This variation observed in apomictic genotypes provides an opportunity to obtain new variants, which may then be included as sources of genetic variability in buffel grass breeding programs. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1353 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA13532018-10-26T11:36:26Z Somatic embryogenesis from in vitro anther culture of apomictic buffel grass genotypes and analysis of regenerated plants using flow cytometry Carloni, Edgardo José Ribotta, Andrea Noemi Lopez Colomba, Eliana Griffa, Sabrina Mariana Quiroga, Mariana Paola Tommasino, Exequiel Arturo Grunberg, Karina Apomixis Cultivo Variación Somaclonal ADN Fitomejoramiento Embrión Somático Cultivation Somaclonal Variation DNA Plant Breeding Somatic Embryos Buffel grass is a fodder grass that reproduces mainly via apomixis. Using in vitro tissue culture in apomictic species provides alternatives that can be applied in a breeding program. The aims of this work were to establish a protocol for in vitro generation of apomictic buffel grass genotypes using anthers as explants and to evaluate the genetic stability of regenerated plants via flow cytometry (FCM). Three genotypes were able to induce embryogenic calli in Murashige and Skoog (MS) medium supplemented with 6 mg/l of 2,4-dichlorophenoxyacetic acid. Seedling regeneration occurred in a MS medium supplemented with 0.5 mg/l napthaleneacetic acid + 1 mg/l 6-benzylaminopurin. Seedlings were derived from somatic embryos and the morphogenic process was induced using the somatic tissue of the stamens. Induction and regeneration efficiency depended on the genotype and was affected by date of tiller collection, different pretreatments or the interaction of these variables. FCM analyses in in vitro regenerated plants showed genetic instability in their nuclear DNA content. Plants with lower nuclear DNA content may indicate DNA aneuploids (8.6 %), whereas plants that had twice the value of nuclear DNA content (4.7 %) suggest in vitro polyploidization. This variation observed in apomictic genotypes provides an opportunity to obtain new variants, which may then be included as sources of genetic variability in buffel grass breeding programs. Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Ribotta, Andrea Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Lopez Colomba, Eliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Griffa, Sabrina Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Quiroga, Mariana Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Tommasino, Exequiel Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina 2017-09-28T17:57:22Z 2017-09-28T17:57:22Z 2014-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1353 https://link.springer.com/article/10.1007/s11240-014-0441-4 0167-6857 1573-5044 (Online version) https://doi.org/10.1007/s11240-014-0441-4 eng info:eu-repo/semantics/restrictedAccess application/pdf Plant cell, tissue and organ culture 117 (3) : 311-322. (2014) |
| spellingShingle | Apomixis Cultivo Variación Somaclonal ADN Fitomejoramiento Embrión Somático Cultivation Somaclonal Variation DNA Plant Breeding Somatic Embryos Carloni, Edgardo José Ribotta, Andrea Noemi Lopez Colomba, Eliana Griffa, Sabrina Mariana Quiroga, Mariana Paola Tommasino, Exequiel Arturo Grunberg, Karina Somatic embryogenesis from in vitro anther culture of apomictic buffel grass genotypes and analysis of regenerated plants using flow cytometry |
| title | Somatic embryogenesis from in vitro anther culture of apomictic buffel grass genotypes and analysis of regenerated plants using flow cytometry |
| title_full | Somatic embryogenesis from in vitro anther culture of apomictic buffel grass genotypes and analysis of regenerated plants using flow cytometry |
| title_fullStr | Somatic embryogenesis from in vitro anther culture of apomictic buffel grass genotypes and analysis of regenerated plants using flow cytometry |
| title_full_unstemmed | Somatic embryogenesis from in vitro anther culture of apomictic buffel grass genotypes and analysis of regenerated plants using flow cytometry |
| title_short | Somatic embryogenesis from in vitro anther culture of apomictic buffel grass genotypes and analysis of regenerated plants using flow cytometry |
| title_sort | somatic embryogenesis from in vitro anther culture of apomictic buffel grass genotypes and analysis of regenerated plants using flow cytometry |
| topic | Apomixis Cultivo Variación Somaclonal ADN Fitomejoramiento Embrión Somático Cultivation Somaclonal Variation DNA Plant Breeding Somatic Embryos |
| url | http://hdl.handle.net/20.500.12123/1353 https://link.springer.com/article/10.1007/s11240-014-0441-4 https://doi.org/10.1007/s11240-014-0441-4 |
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