Evolution of Animal South American RVA Told by the NSP4 Gene E12 Genotype
Rotavirus A (RVA) possesses a genome of 11 double-stranded (ds) RNA segments, and each segment encodes one protein, with the exception of segment 11. NSP4 is a non-structural multifunctional protein encoded by segment 10 that defines the E-genotype. From the 31 E-genotypes described, genotype E12 ha...
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| Format: | Artículo |
| Language: | Inglés |
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MDPI
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/13520 https://www.mdpi.com/1999-4915/14/11/2506 https://doi.org/10.3390/v14112506 |
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| author | Miño, Samuel Badaracco, Alejandra Louge Uriarte, Enrique Leopoldo Ciarlet, Max Parreño, Viviana |
| author_browse | Badaracco, Alejandra Ciarlet, Max Louge Uriarte, Enrique Leopoldo Miño, Samuel Parreño, Viviana |
| author_facet | Miño, Samuel Badaracco, Alejandra Louge Uriarte, Enrique Leopoldo Ciarlet, Max Parreño, Viviana |
| author_sort | Miño, Samuel |
| collection | INTA Digital |
| description | Rotavirus A (RVA) possesses a genome of 11 double-stranded (ds) RNA segments, and each segment encodes one protein, with the exception of segment 11. NSP4 is a non-structural multifunctional protein encoded by segment 10 that defines the E-genotype. From the 31 E-genotypes described, genotype E12 has been described in Argentina, Uruguay, Paraguay, and Brazil in RVA strains infecting different animal species and humans. In this work, we studied the evolutionary relationships of RVA strains carrying the E12 genotype in South America using phylogenetic and phylodynamic approaches. We found that the E12 genotype has a South American origin, with a guanaco (Lama guanicoe) strain as natural host. Interestingly, all the other reported RVA strains carrying the E12 genotype in equine, bovine, caprine, and human strains are related to RVA strains of camelid origin. The evolutionary path and genetic footprint of the E12 genotype were reconstructed starting with the introduction of non-native livestock species into the American continent with the Spanish conquest in the 16th century. The imported animal species were in close contact with South American camelids, and the offspring were exposed to the native RVA strains brought from Europe and the new RVA circulating in guanacos, resulting in the emergence of new RVA strains in the current lineages’ strongly species-specific adaption. In conclusion, we proposed the NSP4 E12 genotype as a genetic geographic marker in the RVA strains circulating in different animal species in South America. |
| format | Artículo |
| id | INTA13520 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA135202022-12-02T12:18:15Z Evolution of Animal South American RVA Told by the NSP4 Gene E12 Genotype Miño, Samuel Badaracco, Alejandra Louge Uriarte, Enrique Leopoldo Ciarlet, Max Parreño, Viviana Filogenia Phylogeny Rotavirus Animal Viruses Virus de los Animales Reordenamiento Genético Evolución en Virus Segmentados Filodinámica Genome Reassortment Evolution in Segmented Viruses Phylodynamic Rotavirus A (RVA) possesses a genome of 11 double-stranded (ds) RNA segments, and each segment encodes one protein, with the exception of segment 11. NSP4 is a non-structural multifunctional protein encoded by segment 10 that defines the E-genotype. From the 31 E-genotypes described, genotype E12 has been described in Argentina, Uruguay, Paraguay, and Brazil in RVA strains infecting different animal species and humans. In this work, we studied the evolutionary relationships of RVA strains carrying the E12 genotype in South America using phylogenetic and phylodynamic approaches. We found that the E12 genotype has a South American origin, with a guanaco (Lama guanicoe) strain as natural host. Interestingly, all the other reported RVA strains carrying the E12 genotype in equine, bovine, caprine, and human strains are related to RVA strains of camelid origin. The evolutionary path and genetic footprint of the E12 genotype were reconstructed starting with the introduction of non-native livestock species into the American continent with the Spanish conquest in the 16th century. The imported animal species were in close contact with South American camelids, and the offspring were exposed to the native RVA strains brought from Europe and the new RVA circulating in guanacos, resulting in the emergence of new RVA strains in the current lineages’ strongly species-specific adaption. In conclusion, we proposed the NSP4 E12 genotype as a genetic geographic marker in the RVA strains circulating in different animal species in South America. EEA Cerro Azul Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina. Fil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Ciarlet, Max. Icosavax. Clinical Development; Estados Unidos Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina 2022-12-02T12:07:32Z 2022-12-02T12:07:32Z 2022-11-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13520 https://www.mdpi.com/1999-4915/14/11/2506 1999-4915 https://doi.org/10.3390/v14112506 eng info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001/2019-PE-E5-I109-001/AR./Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la info:eu-repograntAgreement/INTA/2019-RIST-E5-I111-001/2019-RIST-E5-I111-001/AR./Laboratorios de Diagnóstico Veterinario info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Viruses 14 (11) : 2506. (November 2022) |
| spellingShingle | Filogenia Phylogeny Rotavirus Animal Viruses Virus de los Animales Reordenamiento Genético Evolución en Virus Segmentados Filodinámica Genome Reassortment Evolution in Segmented Viruses Phylodynamic Miño, Samuel Badaracco, Alejandra Louge Uriarte, Enrique Leopoldo Ciarlet, Max Parreño, Viviana Evolution of Animal South American RVA Told by the NSP4 Gene E12 Genotype |
| title | Evolution of Animal South American RVA Told by the NSP4 Gene E12 Genotype |
| title_full | Evolution of Animal South American RVA Told by the NSP4 Gene E12 Genotype |
| title_fullStr | Evolution of Animal South American RVA Told by the NSP4 Gene E12 Genotype |
| title_full_unstemmed | Evolution of Animal South American RVA Told by the NSP4 Gene E12 Genotype |
| title_short | Evolution of Animal South American RVA Told by the NSP4 Gene E12 Genotype |
| title_sort | evolution of animal south american rva told by the nsp4 gene e12 genotype |
| topic | Filogenia Phylogeny Rotavirus Animal Viruses Virus de los Animales Reordenamiento Genético Evolución en Virus Segmentados Filodinámica Genome Reassortment Evolution in Segmented Viruses Phylodynamic |
| url | http://hdl.handle.net/20.500.12123/13520 https://www.mdpi.com/1999-4915/14/11/2506 https://doi.org/10.3390/v14112506 |
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