Effects of deletion of the ac109 gene of Autographa californica nucleopolyhedrovirus on interactions with mammalian cells
Baculoviruses are able to enter into mammalian cells, where they can express a transgene that is placed under an appropriate promoter, without producing infectious progeny. ORF109 encodes an essential baculovirus protein that participates in the interaction of the baculovirus with mammalian cells. T...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1350 https://link.springer.com/article/10.1007/s00705-016-3142-y https://doi.org/10.1007/s00705-016-3142-y |
| _version_ | 1855034789594136576 |
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| author | Alfonso, Victoria Amalfi, Sabrina Lopez, Maria Gabriela Taboga, Oscar Alberto |
| author_browse | Alfonso, Victoria Amalfi, Sabrina Lopez, Maria Gabriela Taboga, Oscar Alberto |
| author_facet | Alfonso, Victoria Amalfi, Sabrina Lopez, Maria Gabriela Taboga, Oscar Alberto |
| author_sort | Alfonso, Victoria |
| collection | INTA Digital |
| description | Baculoviruses are able to enter into mammalian cells, where they can express a transgene that is placed under an appropriate promoter, without producing infectious progeny. ORF109 encodes an essential baculovirus protein that participates in the interaction of the baculovirus with mammalian cells. To date, the mechanisms underlying this interaction are not yet known. We
demonstrated that although a Ac109 knock out virus maintained its ability to enter into BHK-21 cells, there was a marked reduction in the expression efficiency of the nuclear transgene. Moreover, the amount of free cytoplasmic
viral DNA, which was detected by transcription of a reporter gene, was severely diminished. These results suggest Ac109 could be involved in maintaining the
integrity of the viral nucleic acid. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1350 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA13502019-03-22T14:32:14Z Effects of deletion of the ac109 gene of Autographa californica nucleopolyhedrovirus on interactions with mammalian cells Alfonso, Victoria Amalfi, Sabrina Lopez, Maria Gabriela Taboga, Oscar Alberto Genética Autographa Californica Baculovirus Genes Genetics Baculoviruses are able to enter into mammalian cells, where they can express a transgene that is placed under an appropriate promoter, without producing infectious progeny. ORF109 encodes an essential baculovirus protein that participates in the interaction of the baculovirus with mammalian cells. To date, the mechanisms underlying this interaction are not yet known. We demonstrated that although a Ac109 knock out virus maintained its ability to enter into BHK-21 cells, there was a marked reduction in the expression efficiency of the nuclear transgene. Moreover, the amount of free cytoplasmic viral DNA, which was detected by transcription of a reporter gene, was severely diminished. These results suggest Ac109 could be involved in maintaining the integrity of the viral nucleic acid. Inst. de Biotecnología Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Amalfi, Sabrina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-09-28T15:06:24Z 2017-09-28T15:06:24Z 2017-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1350 https://link.springer.com/article/10.1007/s00705-016-3142-y 0304-8608 (Print) 1432-8798 (Online) https://doi.org/10.1007/s00705-016-3142-y eng info:eu-repo/semantics/restrictedAccess application/pdf Archives of virology 162 (3) : 835–840. (March 2017) |
| spellingShingle | Genética Autographa Californica Baculovirus Genes Genetics Alfonso, Victoria Amalfi, Sabrina Lopez, Maria Gabriela Taboga, Oscar Alberto Effects of deletion of the ac109 gene of Autographa californica nucleopolyhedrovirus on interactions with mammalian cells |
| title | Effects of deletion of the ac109 gene of Autographa californica nucleopolyhedrovirus on interactions with mammalian cells |
| title_full | Effects of deletion of the ac109 gene of Autographa californica nucleopolyhedrovirus on interactions with mammalian cells |
| title_fullStr | Effects of deletion of the ac109 gene of Autographa californica nucleopolyhedrovirus on interactions with mammalian cells |
| title_full_unstemmed | Effects of deletion of the ac109 gene of Autographa californica nucleopolyhedrovirus on interactions with mammalian cells |
| title_short | Effects of deletion of the ac109 gene of Autographa californica nucleopolyhedrovirus on interactions with mammalian cells |
| title_sort | effects of deletion of the ac109 gene of autographa californica nucleopolyhedrovirus on interactions with mammalian cells |
| topic | Genética Autographa Californica Baculovirus Genes Genetics |
| url | http://hdl.handle.net/20.500.12123/1350 https://link.springer.com/article/10.1007/s00705-016-3142-y https://doi.org/10.1007/s00705-016-3142-y |
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