Detection of Mycobacterium avium subsp. paratuberculosis and other mycobacteria in retail milk and dairy products in Argentina
Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of paratuberculosis or Johne’s disease (JD), a chronic enteritis disease that affects cattle and other animal species and has been linked to Crohn’s disease, a chronic inflammatory bowel disease in humans. MAP could resist past...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Ediciones INTA
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/13483 |
| _version_ | 1855485181483286528 |
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| author | Cirone, Karina Mariela Morsella, Claudia Graciela Mendez, Laura Luján Paolicchi, Fernando |
| author_browse | Cirone, Karina Mariela Mendez, Laura Luján Morsella, Claudia Graciela Paolicchi, Fernando |
| author_facet | Cirone, Karina Mariela Morsella, Claudia Graciela Mendez, Laura Luján Paolicchi, Fernando |
| author_sort | Cirone, Karina Mariela |
| collection | INTA Digital |
| description | Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of paratuberculosis or Johne’s disease (JD), a chronic enteritis disease that affects cattle and other animal species and has been linked to Crohn’s disease, a chronic inflammatory bowel disease in humans. MAP could resist pasteurization conditions and has been isolated from retail milk in several countries. The aims were to identify MAP and other mycobacteria in retail milk and dairy products in Argentina and to assess the product quality through Mesophilic Total Aerobic Bacteria (TAB) count. Three hundred and eighty-four samples of retail milk were tested for 24 months. All samples were negative for mycobacteria growth. However, 1.56% of the samples were positive for MAP identification by IS900-PCR. The TAB count was higher than the limits established by the Alimentary Argentinian Code (AAC) in 4.95% of the samples. Mycobacteria other than MAP were not detected either by culture or PCR. The MAP positive PCR from retail milk samples would indicate that they could come from dairy farms with JD and suggests that pasteurized milk or dairy products are not significant sources of human exposure to MAP in Argentina. Some milk samples exceeded the limits established by the AAC for TAB counts, indicating that commercialized milk could be processed and stored incorrectly. |
| format | Artículo |
| id | INTA13483 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Ediciones INTA |
| publisherStr | Ediciones INTA |
| record_format | dspace |
| spelling | INTA134832022-11-30T10:18:47Z Detection of Mycobacterium avium subsp. paratuberculosis and other mycobacteria in retail milk and dairy products in Argentina Cirone, Karina Mariela Morsella, Claudia Graciela Mendez, Laura Luján Paolicchi, Fernando Leche Productos Lácteos Control de Calidad Argentina Milk Mycobacterium avium subsp. paratuberculosis Milk Products Quality Control Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of paratuberculosis or Johne’s disease (JD), a chronic enteritis disease that affects cattle and other animal species and has been linked to Crohn’s disease, a chronic inflammatory bowel disease in humans. MAP could resist pasteurization conditions and has been isolated from retail milk in several countries. The aims were to identify MAP and other mycobacteria in retail milk and dairy products in Argentina and to assess the product quality through Mesophilic Total Aerobic Bacteria (TAB) count. Three hundred and eighty-four samples of retail milk were tested for 24 months. All samples were negative for mycobacteria growth. However, 1.56% of the samples were positive for MAP identification by IS900-PCR. The TAB count was higher than the limits established by the Alimentary Argentinian Code (AAC) in 4.95% of the samples. Mycobacteria other than MAP were not detected either by culture or PCR. The MAP positive PCR from retail milk samples would indicate that they could come from dairy farms with JD and suggests that pasteurized milk or dairy products are not significant sources of human exposure to MAP in Argentina. Some milk samples exceeded the limits established by the AAC for TAB counts, indicating that commercialized milk could be processed and stored incorrectly. Mycobacterium avium subesp. paratuberculosis (MAP) es el agente etiológico de la paratuberculosis o la enfermedad de Johne (JD), una enteritis crónica que afecta al ganado vacuno y otras especies animales que se la vincula con la enfermedad de Crohn, una afección intestinal inflamatoria crónica en humanos. MAP podría resistir las condiciones de pasteurización y se lo aisló de la leche al por menor en varios países. Los objetivos fueron identificar MAP y otras micobacterias en leche y productos lácteos al por menor en Argentina y evaluar la calidad de estos a través del conteo de bacterias aerobias totales mesófilas (TAB). Se analizaron 384 muestras de leche de venta minorista durante 24 meses. Todas las muestras resultaron negativas en cuanto al crecimiento de micobacterias. Sin embargo, el 1.56% de las muestras dieron positivas en la identificación de MAP por IS900-PCR. El recuento de TAB superó los límites establecidos por el Código Alimentario Argentino (CAA) en el 4.95% de las muestras. No se detectaron micobacterias distintas de MAP ni por cultivo ni por PCR. La PCR positiva en MAP resultante de muestras de leche al por menor indicaría que podrían provenir de granjas lecheras con JD y sugiere que la leche pasteurizada o los productos lácteos no son fuentes significativas de exposición humana a MAP en Argentina. Algunas muestras de leche excedieron los límites establecidos por la AAC para los recuentos de TAB, lo cual indica que la leche comercializada podría procesarse y almacenarse incorrectamente. EEA Balcarce Fil: Cirone, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Cirone, Karina Mariela. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Mendez, Laura Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. 2022-11-30T10:16:06Z 2022-11-30T10:16:06Z 2022-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13483 1669-2314 0325-8718 eng info:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional. info:eu-repograntAgreement/INTA/PNSA-1115054/AR./Enfermedades parasitarias, infecciosas y tóxico metabólicas que afectan la productividad de los bóvidos para producción de carne y leche. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Argentina .......... (nation) (World, South America) 7006477 Ediciones INTA RIA 48 (3) : 197-203 (noviembre 2022) |
| spellingShingle | Leche Productos Lácteos Control de Calidad Argentina Milk Mycobacterium avium subsp. paratuberculosis Milk Products Quality Control Cirone, Karina Mariela Morsella, Claudia Graciela Mendez, Laura Luján Paolicchi, Fernando Detection of Mycobacterium avium subsp. paratuberculosis and other mycobacteria in retail milk and dairy products in Argentina |
| title | Detection of Mycobacterium avium subsp. paratuberculosis and other mycobacteria in retail milk and dairy products in Argentina |
| title_full | Detection of Mycobacterium avium subsp. paratuberculosis and other mycobacteria in retail milk and dairy products in Argentina |
| title_fullStr | Detection of Mycobacterium avium subsp. paratuberculosis and other mycobacteria in retail milk and dairy products in Argentina |
| title_full_unstemmed | Detection of Mycobacterium avium subsp. paratuberculosis and other mycobacteria in retail milk and dairy products in Argentina |
| title_short | Detection of Mycobacterium avium subsp. paratuberculosis and other mycobacteria in retail milk and dairy products in Argentina |
| title_sort | detection of mycobacterium avium subsp paratuberculosis and other mycobacteria in retail milk and dairy products in argentina |
| topic | Leche Productos Lácteos Control de Calidad Argentina Milk Mycobacterium avium subsp. paratuberculosis Milk Products Quality Control |
| url | http://hdl.handle.net/20.500.12123/13483 |
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