The localization of a heterologous displayed antigen in the baculovirus-budded virion determines the type and strength of induced adaptive immune response
In the search of strategies of presentation of heterologous antigens to elicit humoral or cellular immune responses that modulate and properly potentiate each type of response, researchers have been studying baculovirus (BV) as vaccine vectors with promising results. For some years, several researc...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1348 https://link.springer.com/article/10.1007%2Fs00253-017-8183-y https://doi.org/10.1007/s00253-017-8183-y |
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| author | Tavarone, Maria Eugenia Molina, Guido Nicolas Amalfi, Sabrina Peralta, Andrea Veronica Molinari, Maria Paula Taboga, Oscar Alberto Diaz, Silvio Ricardo Gravisaco, María José |
| author_browse | Amalfi, Sabrina Diaz, Silvio Ricardo Gravisaco, María José Molina, Guido Nicolas Molinari, Maria Paula Peralta, Andrea Veronica Taboga, Oscar Alberto Tavarone, Maria Eugenia |
| author_facet | Tavarone, Maria Eugenia Molina, Guido Nicolas Amalfi, Sabrina Peralta, Andrea Veronica Molinari, Maria Paula Taboga, Oscar Alberto Diaz, Silvio Ricardo Gravisaco, María José |
| author_sort | Tavarone, Maria Eugenia |
| collection | INTA Digital |
| description | In the search of strategies of presentation of heterologous antigens to elicit humoral or cellular immune responses that modulate and properly potentiate each type of response, researchers have been studying baculovirus (BV) as vaccine
vectors with promising results. For some years, several research groups explored different antigen presentation approaches using the BV AcNPV by expressing polypeptides on the surface of budded virions or by de novo synthesis of heterologous antigens by transduction of mammalian cells. In the case of expression on the surface of budded virions, for example, researchers have
expressed polypeptides in peplomers as GP64 glycoprotein fusions or distributed throughout the entire surface by fusions to portions of the G protein of vesicular stomatitis virus, VSV.
Recently, our group developed the strategy of crosspresentation of antigens by fusions ofGP64 to the capsid protein VP39 (capsid display) for the generation of cytotoxic responses.
While the different strategies showed to be effective in raising immune responses, the individuality of each analysis makes difficult the comparison of the results. Here, by comparing the different strategies, we show that localization of the model antigen ovalbumin (OVA) strongly determined the quality and intensity
of the adaptive response to the heterologous antigen.
Furthermore, surface display favored humoral responses,whereas capsid display favored cytotoxic responses. Finally, capsid display showed a much more efficient strategy to activate CD8-mediated responses than transduction. The incorporation
of adjuvants in baculovirus formulations dramatically diminished the immunostimulatory properties of baculovirus. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1348 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA13482019-03-22T14:31:01Z The localization of a heterologous displayed antigen in the baculovirus-budded virion determines the type and strength of induced adaptive immune response Tavarone, Maria Eugenia Molina, Guido Nicolas Amalfi, Sabrina Peralta, Andrea Veronica Molinari, Maria Paula Taboga, Oscar Alberto Diaz, Silvio Ricardo Gravisaco, María José Immunization Immune Response Antigens Genetics Inmunización Respuesta Inmunológica Baculovirus Antígenos In the search of strategies of presentation of heterologous antigens to elicit humoral or cellular immune responses that modulate and properly potentiate each type of response, researchers have been studying baculovirus (BV) as vaccine vectors with promising results. For some years, several research groups explored different antigen presentation approaches using the BV AcNPV by expressing polypeptides on the surface of budded virions or by de novo synthesis of heterologous antigens by transduction of mammalian cells. In the case of expression on the surface of budded virions, for example, researchers have expressed polypeptides in peplomers as GP64 glycoprotein fusions or distributed throughout the entire surface by fusions to portions of the G protein of vesicular stomatitis virus, VSV. Recently, our group developed the strategy of crosspresentation of antigens by fusions ofGP64 to the capsid protein VP39 (capsid display) for the generation of cytotoxic responses. While the different strategies showed to be effective in raising immune responses, the individuality of each analysis makes difficult the comparison of the results. Here, by comparing the different strategies, we show that localization of the model antigen ovalbumin (OVA) strongly determined the quality and intensity of the adaptive response to the heterologous antigen. Furthermore, surface display favored humoral responses,whereas capsid display favored cytotoxic responses. Finally, capsid display showed a much more efficient strategy to activate CD8-mediated responses than transduction. The incorporation of adjuvants in baculovirus formulations dramatically diminished the immunostimulatory properties of baculovirus. Inst. de Biotecnología Fil: Tavarone, Maria Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Molina, Guido Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Amalfi, Sabrina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Peralta, Andrea Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Diaz, Silvio Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-09-28T14:04:52Z 2017-09-28T14:04:52Z 2017-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1348 https://link.springer.com/article/10.1007%2Fs00253-017-8183-y 0175-7598 (Print) 1432-0614 (Online) https://doi.org/10.1007/s00253-017-8183-y eng info:eu-repo/semantics/restrictedAccess application/pdf Applied microbiology and biotechnology 101 (10) : 4175–4184. (May 2017) |
| spellingShingle | Immunization Immune Response Antigens Genetics Inmunización Respuesta Inmunológica Baculovirus Antígenos Tavarone, Maria Eugenia Molina, Guido Nicolas Amalfi, Sabrina Peralta, Andrea Veronica Molinari, Maria Paula Taboga, Oscar Alberto Diaz, Silvio Ricardo Gravisaco, María José The localization of a heterologous displayed antigen in the baculovirus-budded virion determines the type and strength of induced adaptive immune response |
| title | The localization of a heterologous displayed antigen in the baculovirus-budded virion determines the type and strength of induced adaptive immune response |
| title_full | The localization of a heterologous displayed antigen in the baculovirus-budded virion determines the type and strength of induced adaptive immune response |
| title_fullStr | The localization of a heterologous displayed antigen in the baculovirus-budded virion determines the type and strength of induced adaptive immune response |
| title_full_unstemmed | The localization of a heterologous displayed antigen in the baculovirus-budded virion determines the type and strength of induced adaptive immune response |
| title_short | The localization of a heterologous displayed antigen in the baculovirus-budded virion determines the type and strength of induced adaptive immune response |
| title_sort | localization of a heterologous displayed antigen in the baculovirus budded virion determines the type and strength of induced adaptive immune response |
| topic | Immunization Immune Response Antigens Genetics Inmunización Respuesta Inmunológica Baculovirus Antígenos |
| url | http://hdl.handle.net/20.500.12123/1348 https://link.springer.com/article/10.1007%2Fs00253-017-8183-y https://doi.org/10.1007/s00253-017-8183-y |
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