Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera
Varroa destructor Anderson & Trueman (Acari: Varroidae) is one of the major contributors to the significant losses of western honey bee, Apis mellifera L. (Hymenoptera: Apidae), colonies worldwide. Hygienic behavior, in which individual workers detect, uncap, and remove unhealthy brood, is a type of...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Wiley
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/13457 https://onlinelibrary.wiley.com/doi/full/10.1111/eea.13048 https://doi.org/10.1111/eea.13048 |
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| author | Liendo, María Clara Muntaabski, Irina Russo, Romina Maria Lanzavecchia, Silvia Beatriz Segura, Diego Fernando Palacio, María Alejandra Cladera, Jorge Luis Fernández, Patricia Carina Scannapieco, Alejandra Carla |
| author_browse | Cladera, Jorge Luis Fernández, Patricia Carina Lanzavecchia, Silvia Beatriz Liendo, María Clara Muntaabski, Irina Palacio, María Alejandra Russo, Romina Maria Scannapieco, Alejandra Carla Segura, Diego Fernando |
| author_facet | Liendo, María Clara Muntaabski, Irina Russo, Romina Maria Lanzavecchia, Silvia Beatriz Segura, Diego Fernando Palacio, María Alejandra Cladera, Jorge Luis Fernández, Patricia Carina Scannapieco, Alejandra Carla |
| author_sort | Liendo, María Clara |
| collection | INTA Digital |
| description | Varroa destructor Anderson & Trueman (Acari: Varroidae) is one of the major contributors to the significant losses of western honey bee, Apis mellifera L. (Hymenoptera: Apidae), colonies worldwide. Hygienic behavior, in which individual workers detect, uncap, and remove unhealthy brood, is a type of social immunity that reduces pathogen and parasite loads in the colony. Previous evidence suggests that hygienic worker bees identify diseased brood through olfactory cues. The aims of the present work were (1) to study the hygienic behavior of worker bees toward V. destructor-infested cells at different stages of brood development, (2) to explore changes in brood volatile profiles associated with the progression of mite infestation, and (3) to analyze the role of specific volatile compounds in triggering the hygienic behavior. Results showed that the removal rate of infested brood changed along its development and the progression of mite reproduction. Two compounds, ethyl hexanoate and α-pinene, were present in volatile collections from mite-infested pupae but absent from uninfested pupae. Field bioassays showed that these volatiles are relevant to elicit the hygienic behavior. A third compound, β-ocimene, was present in infested and uninfested brood but its abundance pattern varied according to the infestation status throughout brood development. Specifically, for uninfested brood, the abundance of β-ocimene showed a reduction in black-eyed pupae, whereas for infested brood, its abundance decreased drastically in light-pink-eyed pupae and remained constant in black-eyed pupae. Our results revealed that olfactory signals associated with V. destructor infestation change as the reproductive cycle of V. destructor progresses inside the cell. These changes can be mimicked to some extent by adding specific volatile compounds to the cell to induce hygienic removal. These findings shed light on the chemical basis of hygienic behavior against V. destructor and could facilitate the development of improved hygienic selection tools to breed mite-resistant honey bee colonies. |
| format | Artículo |
| id | INTA13457 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA134572022-11-28T10:55:05Z Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera Liendo, María Clara Muntaabski, Irina Russo, Romina Maria Lanzavecchia, Silvia Beatriz Segura, Diego Fernando Palacio, María Alejandra Cladera, Jorge Luis Fernández, Patricia Carina Scannapieco, Alejandra Carla Abeja Melífera Comportamiento Animal Compuesto Volátil Varroa Destructor Apis Melífera Honey Bees Animal Behaviour Volatile Compounds Acarina Hymenoptera Apidae Varroa destructor Anderson & Trueman (Acari: Varroidae) is one of the major contributors to the significant losses of western honey bee, Apis mellifera L. (Hymenoptera: Apidae), colonies worldwide. Hygienic behavior, in which individual workers detect, uncap, and remove unhealthy brood, is a type of social immunity that reduces pathogen and parasite loads in the colony. Previous evidence suggests that hygienic worker bees identify diseased brood through olfactory cues. The aims of the present work were (1) to study the hygienic behavior of worker bees toward V. destructor-infested cells at different stages of brood development, (2) to explore changes in brood volatile profiles associated with the progression of mite infestation, and (3) to analyze the role of specific volatile compounds in triggering the hygienic behavior. Results showed that the removal rate of infested brood changed along its development and the progression of mite reproduction. Two compounds, ethyl hexanoate and α-pinene, were present in volatile collections from mite-infested pupae but absent from uninfested pupae. Field bioassays showed that these volatiles are relevant to elicit the hygienic behavior. A third compound, β-ocimene, was present in infested and uninfested brood but its abundance pattern varied according to the infestation status throughout brood development. Specifically, for uninfested brood, the abundance of β-ocimene showed a reduction in black-eyed pupae, whereas for infested brood, its abundance decreased drastically in light-pink-eyed pupae and remained constant in black-eyed pupae. Our results revealed that olfactory signals associated with V. destructor infestation change as the reproductive cycle of V. destructor progresses inside the cell. These changes can be mimicked to some extent by adding specific volatile compounds to the cell to induce hygienic removal. These findings shed light on the chemical basis of hygienic behavior against V. destructor and could facilitate the development of improved hygienic selection tools to breed mite-resistant honey bee colonies. EEA Balcarce Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Russo, Romina María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Palacio, María Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Fernández, Patricia Carina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Fil: Fernández, Patricia Carina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. 2022-11-28T10:51:26Z 2022-11-28T10:51:26Z 2021-03-16 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13457 https://onlinelibrary.wiley.com/doi/full/10.1111/eea.13048 1570-7458 https://doi.org/10.1111/eea.13048 eng info:eu-repograntAgreement/INTA/PNAPI-1112042/AR./Estrategias multidisciplinarias para mitigar el efecto del nuevo contexto ambiental y productivo sobre la colmena. info:eu-repo/semantics/restrictedAccess application/pdf Wiley Entomologia Experimentalis et Applicata 169 : 563–574 (June 2021) |
| spellingShingle | Abeja Melífera Comportamiento Animal Compuesto Volátil Varroa Destructor Apis Melífera Honey Bees Animal Behaviour Volatile Compounds Acarina Hymenoptera Apidae Liendo, María Clara Muntaabski, Irina Russo, Romina Maria Lanzavecchia, Silvia Beatriz Segura, Diego Fernando Palacio, María Alejandra Cladera, Jorge Luis Fernández, Patricia Carina Scannapieco, Alejandra Carla Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera |
| title | Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera |
| title_full | Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera |
| title_fullStr | Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera |
| title_full_unstemmed | Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera |
| title_short | Temporal changes in volatile profiles of Varroa destructor-infested brood may trigger hygienic behavior in Apis mellifera |
| title_sort | temporal changes in volatile profiles of varroa destructor infested brood may trigger hygienic behavior in apis mellifera |
| topic | Abeja Melífera Comportamiento Animal Compuesto Volátil Varroa Destructor Apis Melífera Honey Bees Animal Behaviour Volatile Compounds Acarina Hymenoptera Apidae |
| url | http://hdl.handle.net/20.500.12123/13457 https://onlinelibrary.wiley.com/doi/full/10.1111/eea.13048 https://doi.org/10.1111/eea.13048 |
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