Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection
Tomato chlorosis virus (ToCV) is a whitefly-transmitted crinivirus that causes yield losses, mainly in tomato (Solanum lycopersicum) and potato (S. tuberosum) crops. In this work, a polyclonal antiserum for detecting the virus using a dot-blot immunoassay was developed and the responses of criniviru...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
Springer
2022
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/13456 https://link.springer.com/article/10.1007/s40858-020-00379-0 https://doi.org/10.1007/s40858-020-00379-0 |
| _version_ | 1855485171966410752 |
|---|---|
| author | Pinto, Luiz R. Bampi, Daiana Silva, Talita N. Edwards Molina, Juan Pablo Kitajima, Elliot W. Rezende, Jorge A. Bergamin Filho, Armando |
| author_browse | Bampi, Daiana Bergamin Filho, Armando Edwards Molina, Juan Pablo Kitajima, Elliot W. Pinto, Luiz R. Rezende, Jorge A. Silva, Talita N. |
| author_facet | Pinto, Luiz R. Bampi, Daiana Silva, Talita N. Edwards Molina, Juan Pablo Kitajima, Elliot W. Rezende, Jorge A. Bergamin Filho, Armando |
| author_sort | Pinto, Luiz R. |
| collection | INTA Digital |
| description | Tomato chlorosis virus (ToCV) is a whitefly-transmitted crinivirus that causes yield losses, mainly in tomato (Solanum lycopersicum) and potato (S. tuberosum) crops. In this work, a polyclonal antiserum for detecting the virus using a dot-blot immunoassay was developed and the responses of crinivirus-infected potato genotypes were evaluated. The virus was purified using infected tomato leaves and the antiserum was obtained by intramuscular injection of purified viral preparations in rabbit. Tomato and potato tissue samples were used to validate the polyclonal antiserum for detection of ToCV, previously analyzed by RT-PCR. A total of 81 tomato and potato samples were analyzed by RT-PCR and dot-blot immunoassay (DBIA). All samples were positive in RT-PCR, but three of them did not react in DBIA, showing of 96.3% efficiency compared with that in RT-PCR. All potato genotypes inoculated with ToCV by Bemisia tabaci MEAM1 were susceptible to infection. Potato plants of cv. Camila infected by ToCV showed the lowest virus titer and were asymptomatic. |
| format | Artículo |
| id | INTA13456 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA134562022-11-28T10:44:07Z Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection Pinto, Luiz R. Bampi, Daiana Silva, Talita N. Edwards Molina, Juan Pablo Kitajima, Elliot W. Rezende, Jorge A. Bergamin Filho, Armando Crinivirus Papa Tomate Virus de las Plantas Clorosis Potatoes Tomatoes Plant Viruses Chlorosis Tomato chlorosis virus (ToCV) is a whitefly-transmitted crinivirus that causes yield losses, mainly in tomato (Solanum lycopersicum) and potato (S. tuberosum) crops. In this work, a polyclonal antiserum for detecting the virus using a dot-blot immunoassay was developed and the responses of crinivirus-infected potato genotypes were evaluated. The virus was purified using infected tomato leaves and the antiserum was obtained by intramuscular injection of purified viral preparations in rabbit. Tomato and potato tissue samples were used to validate the polyclonal antiserum for detection of ToCV, previously analyzed by RT-PCR. A total of 81 tomato and potato samples were analyzed by RT-PCR and dot-blot immunoassay (DBIA). All samples were positive in RT-PCR, but three of them did not react in DBIA, showing of 96.3% efficiency compared with that in RT-PCR. All potato genotypes inoculated with ToCV by Bemisia tabaci MEAM1 were susceptible to infection. Potato plants of cv. Camila infected by ToCV showed the lowest virus titer and were asymptomatic. EEA Balcarce Fil: Pinto, Luiz R. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil. Fil: Bampi, Daiana. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil. Fil: Silva, Talita N. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil. Fil: Edwards Molina, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Kitajima, Elliot W. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil. Fil: Rezende, Jorge A. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil. Fil: Bergamin Filho, Armando. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil. 2022-11-28T10:40:59Z 2022-11-28T10:40:59Z 2021-03-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13456 https://link.springer.com/article/10.1007/s40858-020-00379-0 1983-2052 https://doi.org/10.1007/s40858-020-00379-0 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Tropical Plant Pathology 46 : 156-162 (March 2021) |
| spellingShingle | Crinivirus Papa Tomate Virus de las Plantas Clorosis Potatoes Tomatoes Plant Viruses Chlorosis Pinto, Luiz R. Bampi, Daiana Silva, Talita N. Edwards Molina, Juan Pablo Kitajima, Elliot W. Rezende, Jorge A. Bergamin Filho, Armando Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection |
| title | Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection |
| title_full | Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection |
| title_fullStr | Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection |
| title_full_unstemmed | Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection |
| title_short | Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection |
| title_sort | dot blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection |
| topic | Crinivirus Papa Tomate Virus de las Plantas Clorosis Potatoes Tomatoes Plant Viruses Chlorosis |
| url | http://hdl.handle.net/20.500.12123/13456 https://link.springer.com/article/10.1007/s40858-020-00379-0 https://doi.org/10.1007/s40858-020-00379-0 |
| work_keys_str_mv | AT pintoluizr dotblotimmunoassayfordetectionoftomatochlorosisvirusandreactionofpotatogenotypestovirusinfection AT bampidaiana dotblotimmunoassayfordetectionoftomatochlorosisvirusandreactionofpotatogenotypestovirusinfection AT silvatalitan dotblotimmunoassayfordetectionoftomatochlorosisvirusandreactionofpotatogenotypestovirusinfection AT edwardsmolinajuanpablo dotblotimmunoassayfordetectionoftomatochlorosisvirusandreactionofpotatogenotypestovirusinfection AT kitajimaelliotw dotblotimmunoassayfordetectionoftomatochlorosisvirusandreactionofpotatogenotypestovirusinfection AT rezendejorgea dotblotimmunoassayfordetectionoftomatochlorosisvirusandreactionofpotatogenotypestovirusinfection AT bergaminfilhoarmando dotblotimmunoassayfordetectionoftomatochlorosisvirusandreactionofpotatogenotypestovirusinfection |