Association of TNF-α gene promoter region polymorphisms in bovine leukemia virus (BLV)-infected cattle with different proviral loads
Tumor necrosis factor alpha (TNF-α) is a pleiotropic cytokine involved in the immune response against viral and other infections. Its expression levels are affected by a polymorphism in the promoter region of the gene. Bovine leukemia virus is a retrovirus that infects cattle and develops two differ...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1344 https://link.springer.com/article/10.1007/s00705-015-2448-5 https://doi.org/10.1007/s00705-015-2448-5 |
| _version_ | 1855482872278810624 |
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| author | Lendez, Pamela Anahi Passucci, Juan Antonio Poli, Mario Andres Gutierrez, Silvina Elena Dolcini, Guillermina Laura Ceriani, Maria Carolina |
| author_browse | Ceriani, Maria Carolina Dolcini, Guillermina Laura Gutierrez, Silvina Elena Lendez, Pamela Anahi Passucci, Juan Antonio Poli, Mario Andres |
| author_facet | Lendez, Pamela Anahi Passucci, Juan Antonio Poli, Mario Andres Gutierrez, Silvina Elena Dolcini, Guillermina Laura Ceriani, Maria Carolina |
| author_sort | Lendez, Pamela Anahi |
| collection | INTA Digital |
| description | Tumor necrosis factor alpha (TNF-α) is a pleiotropic cytokine involved in the immune response against viral and other infections. Its expression levels are affected by a polymorphism in the promoter region of the gene. Bovine leukemia virus is a retrovirus that infects cattle and develops two different infection profiles in the host. One profile is characterized by a high number of proviral copies integrated into the host genome and a strong immune response against the virus, while the most relevant property of the other profile is that the number of copies integrated into the host genome is almost undetectable and the immune response is very weak. We selected a population of cattle sufficiently large for statistical analysis and classified them according to whether they had a high or low proviral load (HPL or LPL). Polymorphisms in the promoter region were identified by PCR-RFLP. The results indicated that, in the HPL group, the three possible genotypes were normally distributed and that, in the LPL group, there was a significant association between the proviral load and a low frequency of the G/G genotype at position -824. |
| format | Artículo |
| id | INTA1344 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA13442019-05-17T12:43:43Z Association of TNF-α gene promoter region polymorphisms in bovine leukemia virus (BLV)-infected cattle with different proviral loads Lendez, Pamela Anahi Passucci, Juan Antonio Poli, Mario Andres Gutierrez, Silvina Elena Dolcini, Guillermina Laura Ceriani, Maria Carolina Ganado Bovino Virus Leucemia Bovina Polimorfismo Genética Cattle Bovine Leukaemia Virus Polymorphism Genetics Tumor necrosis factor alpha (TNF-α) is a pleiotropic cytokine involved in the immune response against viral and other infections. Its expression levels are affected by a polymorphism in the promoter region of the gene. Bovine leukemia virus is a retrovirus that infects cattle and develops two different infection profiles in the host. One profile is characterized by a high number of proviral copies integrated into the host genome and a strong immune response against the virus, while the most relevant property of the other profile is that the number of copies integrated into the host genome is almost undetectable and the immune response is very weak. We selected a population of cattle sufficiently large for statistical analysis and classified them according to whether they had a high or low proviral load (HPL or LPL). Polymorphisms in the promoter region were identified by PCR-RFLP. The results indicated that, in the HPL group, the three possible genotypes were normally distributed and that, in the LPL group, there was a significant association between the proviral load and a low frequency of the G/G genotype at position -824. Inst. de Genética "Ewald A. Favret"- IGEAF Fil: Lendez, Pamela Anahí. Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina Fil: Passucci, Juan Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet- Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Gutiérrez, Silvina Elena. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina Fil: Dolcini, Guillermina Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina Fil: Ceriani, Maria Carolina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina 2017-09-28T12:00:13Z 2017-09-28T12:00:13Z 2015-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1344 https://link.springer.com/article/10.1007/s00705-015-2448-5 0304-8608 (Print) 1432-8798 (Online) https://doi.org/10.1007/s00705-015-2448-5 eng info:eu-repo/semantics/restrictedAccess application/pdf Archives of virology 160 (8) : 2001–2007. (August 2015) |
| spellingShingle | Ganado Bovino Virus Leucemia Bovina Polimorfismo Genética Cattle Bovine Leukaemia Virus Polymorphism Genetics Lendez, Pamela Anahi Passucci, Juan Antonio Poli, Mario Andres Gutierrez, Silvina Elena Dolcini, Guillermina Laura Ceriani, Maria Carolina Association of TNF-α gene promoter region polymorphisms in bovine leukemia virus (BLV)-infected cattle with different proviral loads |
| title | Association of TNF-α gene promoter region polymorphisms in bovine leukemia virus (BLV)-infected cattle with different proviral loads |
| title_full | Association of TNF-α gene promoter region polymorphisms in bovine leukemia virus (BLV)-infected cattle with different proviral loads |
| title_fullStr | Association of TNF-α gene promoter region polymorphisms in bovine leukemia virus (BLV)-infected cattle with different proviral loads |
| title_full_unstemmed | Association of TNF-α gene promoter region polymorphisms in bovine leukemia virus (BLV)-infected cattle with different proviral loads |
| title_short | Association of TNF-α gene promoter region polymorphisms in bovine leukemia virus (BLV)-infected cattle with different proviral loads |
| title_sort | association of tnf α gene promoter region polymorphisms in bovine leukemia virus blv infected cattle with different proviral loads |
| topic | Ganado Bovino Virus Leucemia Bovina Polimorfismo Genética Cattle Bovine Leukaemia Virus Polymorphism Genetics |
| url | http://hdl.handle.net/20.500.12123/1344 https://link.springer.com/article/10.1007/s00705-015-2448-5 https://doi.org/10.1007/s00705-015-2448-5 |
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