Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation
Escherichia coli is the bacteria most commonly used as an indicator of fecal contamination in agricultural environments. Moreover, E. coli is categorized as a priority pathogen due to its widespread antibiotic resistance. This study aimed to characterize E. coli strains isolated from 10 horticultura...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/13375 https://link.springer.com/article/10.1007/s11356-021-17523-1 https://doi.org/10.1007/s11356-021-17523-1 |
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| author | Pellegrini, María Celeste Okada, Elena Gonzalez Pasayo, Ramon Alejandro Ponce, Alejandra Graciela |
| author_browse | Gonzalez Pasayo, Ramon Alejandro Okada, Elena Pellegrini, María Celeste Ponce, Alejandra Graciela |
| author_facet | Pellegrini, María Celeste Okada, Elena Gonzalez Pasayo, Ramon Alejandro Ponce, Alejandra Graciela |
| author_sort | Pellegrini, María Celeste |
| collection | INTA Digital |
| description | Escherichia coli is the bacteria most commonly used as an indicator of fecal contamination in agricultural environments. Moreover, E. coli is categorized as a priority pathogen due to its widespread antibiotic resistance. This study aimed to characterize E. coli strains isolated from 10 horticultural farms. Isolates were obtained from samples of vegetable crops (n = 62), the surrounding soil (n = 62), poultry litter (n = 8), and groundwater (n = 6). Phyllo-grouping assignment was performed on the total of E. coli isolates. Antibiograms and quantification of the minimal inhibitory concentration (MIC) were performed with antibiotics commonly used in humans. Biofilm formation capacity was studied by quantifying cells attached to culture tubes. Overall, 21 E. coli isolates were obtained. Three phylogenetic groups (A, B1, and C) and two Escherichia clade IV and IV–V were identified in the collection by polymerase chain reaction. Sixty-seven percent of the E. coli isolates were resistant to amoxicillin-clavulanic acid and/or ampicillin. Amoxicillin MIC values ranged from 11.9 to >190.5 µg/mL and ampicillin MIC values ranged from 3 to >190.5 µg/mL. All the E. coli isolates, resistant and non-resistant, had biofilm forming capacity. The presence of phenotypic resistance on fresh produce and environmental matrices could present significant opportunities for contamination that result in health risks for consumers. To the authors’ best knowledge, this is the first environmental assessment of resistant E. coli occurrence in horticultural farms in South America. |
| format | info:ar-repo/semantics/artículo |
| id | INTA13375 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA133752022-11-10T14:28:54Z Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation Pellegrini, María Celeste Okada, Elena Gonzalez Pasayo, Ramon Alejandro Ponce, Alejandra Graciela Escherichia Coli Suelo Aguas Subterráneas Cama (animales) Antibióticos Biofilmes (microbiología) Soil Groundwater Litter for Animals Antibiotics Biofilms (microbiology) Escherichia coli is the bacteria most commonly used as an indicator of fecal contamination in agricultural environments. Moreover, E. coli is categorized as a priority pathogen due to its widespread antibiotic resistance. This study aimed to characterize E. coli strains isolated from 10 horticultural farms. Isolates were obtained from samples of vegetable crops (n = 62), the surrounding soil (n = 62), poultry litter (n = 8), and groundwater (n = 6). Phyllo-grouping assignment was performed on the total of E. coli isolates. Antibiograms and quantification of the minimal inhibitory concentration (MIC) were performed with antibiotics commonly used in humans. Biofilm formation capacity was studied by quantifying cells attached to culture tubes. Overall, 21 E. coli isolates were obtained. Three phylogenetic groups (A, B1, and C) and two Escherichia clade IV and IV–V were identified in the collection by polymerase chain reaction. Sixty-seven percent of the E. coli isolates were resistant to amoxicillin-clavulanic acid and/or ampicillin. Amoxicillin MIC values ranged from 11.9 to >190.5 µg/mL and ampicillin MIC values ranged from 3 to >190.5 µg/mL. All the E. coli isolates, resistant and non-resistant, had biofilm forming capacity. The presence of phenotypic resistance on fresh produce and environmental matrices could present significant opportunities for contamination that result in health risks for consumers. To the authors’ best knowledge, this is the first environmental assessment of resistant E. coli occurrence in horticultural farms in South America. EEA Balcarce Fil: Pellegrini, María Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Okada, Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: González Pasayo, Ramón Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Ponce, Alejandra Graciela. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Comisión de Investigaciones Científicas; Argentina. Instituto de Ciencia y Tecnología de los alimentos; Argentina. Fil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. 2022-11-10T14:21:40Z 2022-11-10T14:21:40Z 2022-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13375 https://link.springer.com/article/10.1007/s11356-021-17523-1 1614-7499 https://doi.org/10.1007/s11356-021-17523-1 eng info:eu-repograntAgreement/INTA/2019-PD-E2-I518-002/2019-PD-E2-I518-002/AR./Estudio del impacto ambiental, gestión y tratamiento de residuos y efluentes sobre sistemas agropecuarios y agroindustriales para su valorización agronómica info:eu-repo/semantics/restrictedAccess application/pdf Springer Environmental Science and Pollution Research 29 : 23225-23236 (2022) |
| spellingShingle | Escherichia Coli Suelo Aguas Subterráneas Cama (animales) Antibióticos Biofilmes (microbiología) Soil Groundwater Litter for Animals Antibiotics Biofilms (microbiology) Pellegrini, María Celeste Okada, Elena Gonzalez Pasayo, Ramon Alejandro Ponce, Alejandra Graciela Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation |
| title | Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation |
| title_full | Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation |
| title_fullStr | Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation |
| title_full_unstemmed | Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation |
| title_short | Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation |
| title_sort | prevalence of escherichia coli strains in horticultural farms from argentina antibiotic resistance biofilm formation and phylogenetic affiliation |
| topic | Escherichia Coli Suelo Aguas Subterráneas Cama (animales) Antibióticos Biofilmes (microbiología) Soil Groundwater Litter for Animals Antibiotics Biofilms (microbiology) |
| url | http://hdl.handle.net/20.500.12123/13375 https://link.springer.com/article/10.1007/s11356-021-17523-1 https://doi.org/10.1007/s11356-021-17523-1 |
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