Study of the dynamics of in vitro infection with bovine gammaherpesvirus type 4 and apoptosis markers in susceptible cells
Bovine gammaherpesvirus type 4 (BoHV-4) shows tropism for the endometrium, in which it causes the death of epithelial and stroma cells. Despite having anti-apoptotic genes in its genome, experiments based on immortalized cell lines have shown that BoHV-4 induces cell death by apoptosis. In the prese...
| Main Authors: | , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2022
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/13359 https://www.sciencedirect.com/science/article/pii/S0882401022002583 https://doi.org/10.1016/j.micpath.2022.105645 |
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| author | Romeo, Florencia Delgado, Santiago Germán Louge Uriarte, Enrique Leopoldo Storani, Leonardo Martínez Cuesta, Lucia Morán, Pedro González Altamiranda, Erika Odeon, Anselmo Carlos Pérez, Sandra |
| author_browse | Delgado, Santiago Germán González Altamiranda, Erika Louge Uriarte, Enrique Leopoldo Martínez Cuesta, Lucia Morán, Pedro Odeon, Anselmo Carlos Pérez, Sandra Romeo, Florencia Storani, Leonardo |
| author_facet | Romeo, Florencia Delgado, Santiago Germán Louge Uriarte, Enrique Leopoldo Storani, Leonardo Martínez Cuesta, Lucia Morán, Pedro González Altamiranda, Erika Odeon, Anselmo Carlos Pérez, Sandra |
| author_sort | Romeo, Florencia |
| collection | INTA Digital |
| description | Bovine gammaherpesvirus type 4 (BoHV-4) shows tropism for the endometrium, in which it causes the death of epithelial and stroma cells. Despite having anti-apoptotic genes in its genome, experiments based on immortalized cell lines have shown that BoHV-4 induces cell death by apoptosis. In the present study, we evaluated BoHV-4 replication, pro-apoptotic (Bax) and anti-apoptotic (Bcl-2) mitochondrial genes expression and chromatin condensation in bovine endometrium primary culture cells (BEC) and in the Madin Darby bovine kidney (MDBK) cell line. Results showed that BoHV-4 has a preference for replication in BEC cells over the MDBK cell line, demonstrated by the high viral titer that is consistent with the tropism of the virus. In BEC cells, chromatin condensation was consistent with the values of viral kinetics at the late stage of infection, accompanied with a balance in the mRNA levels of apoptotic mitochondrial proteins. As a consequence, in those cells viral transmission would be enhanced by inhibiting apoptosis in the early stage of virus proliferation, allowing the complete production of viral progeny, and then, the induction of apoptosis in late stages would allow neighboring cells infection. In MDBK cells replication kinetics was coincident with the up-regulation of Bcl-2, which suggests that the productive infection in MDBK is associated with a lytic phase of the virus or another cell death pathway (probably autophagy mechanism) at the late stage of infection. The results agree with the study of nuclear morphology, where a constant chromatin condensation was observed over time. It is clear that the documented BoHV-4 apoptotic responses observed in the cell lines studied above are not valid in cells from primary cultures. The data presented in this study suggest that BoHV-4 could induce apoptosis in BEC cells without a leading role of the mitochondria pathway. Further studies will be necessary to characterize in detail the programmed cell death pathways involved in BoHV-4 infection in the primary cell cultures evaluated. |
| format | info:ar-repo/semantics/artículo |
| id | INTA13359 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA133592022-11-09T14:19:28Z Study of the dynamics of in vitro infection with bovine gammaherpesvirus type 4 and apoptosis markers in susceptible cells Romeo, Florencia Delgado, Santiago Germán Louge Uriarte, Enrique Leopoldo Storani, Leonardo Martínez Cuesta, Lucia Morán, Pedro González Altamiranda, Erika Odeon, Anselmo Carlos Pérez, Sandra Cepas Herpes Virus Bovino Argentina Enfermedades de los Animales Strains Bovine Herpesvirus In Vitro Animal Diseases Bovine gammaherpesvirus type 4 (BoHV-4) shows tropism for the endometrium, in which it causes the death of epithelial and stroma cells. Despite having anti-apoptotic genes in its genome, experiments based on immortalized cell lines have shown that BoHV-4 induces cell death by apoptosis. In the present study, we evaluated BoHV-4 replication, pro-apoptotic (Bax) and anti-apoptotic (Bcl-2) mitochondrial genes expression and chromatin condensation in bovine endometrium primary culture cells (BEC) and in the Madin Darby bovine kidney (MDBK) cell line. Results showed that BoHV-4 has a preference for replication in BEC cells over the MDBK cell line, demonstrated by the high viral titer that is consistent with the tropism of the virus. In BEC cells, chromatin condensation was consistent with the values of viral kinetics at the late stage of infection, accompanied with a balance in the mRNA levels of apoptotic mitochondrial proteins. As a consequence, in those cells viral transmission would be enhanced by inhibiting apoptosis in the early stage of virus proliferation, allowing the complete production of viral progeny, and then, the induction of apoptosis in late stages would allow neighboring cells infection. In MDBK cells replication kinetics was coincident with the up-regulation of Bcl-2, which suggests that the productive infection in MDBK is associated with a lytic phase of the virus or another cell death pathway (probably autophagy mechanism) at the late stage of infection. The results agree with the study of nuclear morphology, where a constant chromatin condensation was observed over time. It is clear that the documented BoHV-4 apoptotic responses observed in the cell lines studied above are not valid in cells from primary cultures. The data presented in this study suggest that BoHV-4 could induce apoptosis in BEC cells without a leading role of the mitochondria pathway. Further studies will be necessary to characterize in detail the programmed cell death pathways involved in BoHV-4 infection in the primary cell cultures evaluated. EEA Balcarce Fil: Romeo, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Romeo, Florencia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Delgado, Santiago. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Louge Uriarte, Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Storani, Leonardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Storani, Leonardo. Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación; Argentina. Fil: Martínez Cuesta, Lucia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Martínez Cuesta, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Morán, Pedro. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Fil: González Altamiranda, Erika. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Odeón, Anselmo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Pérez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Verna, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. 2022-11-09T14:15:11Z 2022-11-09T14:15:11Z 2022-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13359 https://www.sciencedirect.com/science/article/pii/S0882401022002583 0882-4010 https://doi.org/10.1016/j.micpath.2022.105645 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Microbial Pathogenesis 169 : 105645 (August 2022) |
| spellingShingle | Cepas Herpes Virus Bovino Argentina Enfermedades de los Animales Strains Bovine Herpesvirus In Vitro Animal Diseases Romeo, Florencia Delgado, Santiago Germán Louge Uriarte, Enrique Leopoldo Storani, Leonardo Martínez Cuesta, Lucia Morán, Pedro González Altamiranda, Erika Odeon, Anselmo Carlos Pérez, Sandra Study of the dynamics of in vitro infection with bovine gammaherpesvirus type 4 and apoptosis markers in susceptible cells |
| title | Study of the dynamics of in vitro infection with bovine gammaherpesvirus type 4 and apoptosis markers in susceptible cells |
| title_full | Study of the dynamics of in vitro infection with bovine gammaherpesvirus type 4 and apoptosis markers in susceptible cells |
| title_fullStr | Study of the dynamics of in vitro infection with bovine gammaherpesvirus type 4 and apoptosis markers in susceptible cells |
| title_full_unstemmed | Study of the dynamics of in vitro infection with bovine gammaherpesvirus type 4 and apoptosis markers in susceptible cells |
| title_short | Study of the dynamics of in vitro infection with bovine gammaherpesvirus type 4 and apoptosis markers in susceptible cells |
| title_sort | study of the dynamics of in vitro infection with bovine gammaherpesvirus type 4 and apoptosis markers in susceptible cells |
| topic | Cepas Herpes Virus Bovino Argentina Enfermedades de los Animales Strains Bovine Herpesvirus In Vitro Animal Diseases |
| url | http://hdl.handle.net/20.500.12123/13359 https://www.sciencedirect.com/science/article/pii/S0882401022002583 https://doi.org/10.1016/j.micpath.2022.105645 |
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