Pre-Selection test to identify high responder donor goats
The aim of the study was to evaluate the feasibility of pre-selection of high or low responder does prior to the superovulatory protocols. Twenty Saanen does received 800 IU of equine chorionic gonadotropin (eCG) at the end of long-term progestogen treatment. Fourteen days later, a second progestoge...
| Autores principales: | , , , , , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1324 http://onlinelibrary.wiley.com/doi/10.1111/rda.12690/full |
| _version_ | 1855034785673510912 |
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| author | Cueto, Marcela Isabel Gibbons, Alejandro Eduardo Balaro, Mario Felipe Alvarez Brandao, Felipe Zandonadi Maia, A.L.R.S. Souza-Fabjan, Joanna Maria Gonçalves Fonseca, Jeferson Ferreira |
| author_browse | Balaro, Mario Felipe Alvarez Brandao, Felipe Zandonadi Cueto, Marcela Isabel Fonseca, Jeferson Ferreira Gibbons, Alejandro Eduardo Maia, A.L.R.S. Souza-Fabjan, Joanna Maria Gonçalves |
| author_facet | Cueto, Marcela Isabel Gibbons, Alejandro Eduardo Balaro, Mario Felipe Alvarez Brandao, Felipe Zandonadi Maia, A.L.R.S. Souza-Fabjan, Joanna Maria Gonçalves Fonseca, Jeferson Ferreira |
| author_sort | Cueto, Marcela Isabel |
| collection | INTA Digital |
| description | The aim of the study was to evaluate the feasibility of pre-selection of high or low responder does prior to the superovulatory protocols. Twenty Saanen does received 800 IU of equine chorionic gonadotropin (eCG) at the end of long-term progestogen treatment. Fourteen days later, a second progestogen protocol associated with a multiple-dose follicle stimulation hormone (FSH) treatment (5 IU/kg of FSH, in six decreasing doses between days 4 to 6 of the protocol) was administered. Transrectal ultrasound was used to assess the follicular status at the beginning of superovulatory treatments, at the oestrous onset and on the seventh day of the oestrous cycle for counting corpora lutea (CL). A significant lower number of CL was obtained in eCG-treated in comparision with FSH-treated does (p < 0.05). A quartic regression was able to explain the relationship between the number of CL in response to both treatments (r2=0.50; p < 0.05). Seventy per cent (14 of 20) of does maintained the same ovulatory response (high or low) after treatments. The Kappa (κ = 0.40; p < 0.05) and Spearman (rs = 0.39; p = 0.08) coefficients were able to show a relationship between treatments. Regarding the follicular status, there is a significant relationship between the number of small follicles (r = 0.71; r2=0.47; p < 0.01) and total follicles (r = 0.60; p < 0.01) at eCG and first FSH dose with the number of CL. Moreover, it was found a negative relationship between the presence of large follicles and the number of CL in response to eCG treatment (r = −0.44; p < 0.05), but not from FSH (p > 0.05). In conclusion, the screening test with eCG has the potential to identify Saanen does that will better respond to the superovulatory protocol with FSH. In addition, it highlighted the importance of an ultrasound evaluation prior to the beginning of superovulatory treatments with FSH to characterize the follicular status and identify the potential donors of high ovulatory response in MOET programmes in goats. |
| format | info:eu-repo/semantics/article |
| id | INTA1324 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA13242018-01-11T15:41:55Z Pre-Selection test to identify high responder donor goats Cueto, Marcela Isabel Gibbons, Alejandro Eduardo Balaro, Mario Felipe Alvarez Brandao, Felipe Zandonadi Maia, A.L.R.S. Souza-Fabjan, Joanna Maria Gonçalves Fonseca, Jeferson Ferreira Producción Animal Caprinos Reproducción Animal Production Goats Reproduction The aim of the study was to evaluate the feasibility of pre-selection of high or low responder does prior to the superovulatory protocols. Twenty Saanen does received 800 IU of equine chorionic gonadotropin (eCG) at the end of long-term progestogen treatment. Fourteen days later, a second progestogen protocol associated with a multiple-dose follicle stimulation hormone (FSH) treatment (5 IU/kg of FSH, in six decreasing doses between days 4 to 6 of the protocol) was administered. Transrectal ultrasound was used to assess the follicular status at the beginning of superovulatory treatments, at the oestrous onset and on the seventh day of the oestrous cycle for counting corpora lutea (CL). A significant lower number of CL was obtained in eCG-treated in comparision with FSH-treated does (p < 0.05). A quartic regression was able to explain the relationship between the number of CL in response to both treatments (r2=0.50; p < 0.05). Seventy per cent (14 of 20) of does maintained the same ovulatory response (high or low) after treatments. The Kappa (κ = 0.40; p < 0.05) and Spearman (rs = 0.39; p = 0.08) coefficients were able to show a relationship between treatments. Regarding the follicular status, there is a significant relationship between the number of small follicles (r = 0.71; r2=0.47; p < 0.01) and total follicles (r = 0.60; p < 0.01) at eCG and first FSH dose with the number of CL. Moreover, it was found a negative relationship between the presence of large follicles and the number of CL in response to eCG treatment (r = −0.44; p < 0.05), but not from FSH (p > 0.05). In conclusion, the screening test with eCG has the potential to identify Saanen does that will better respond to the superovulatory protocol with FSH. In addition, it highlighted the importance of an ultrasound evaluation prior to the beginning of superovulatory treatments with FSH to characterize the follicular status and identify the potential donors of high ovulatory response in MOET programmes in goats. EEA Bariloche Fil: Balaro, Mario Felipe Alvarez. Universidade Federal Fluminense. Faculdade de Veterinária. Departamento de Patologia e Clínica Veterinaria; Brasil Fil: Brandao, Felipe Zandonadi. Universidade Federal Fluminense. Faculdade de Veterinária. Departamento de Patologia e Clínica Veterinaria; Brasil Fil: Maia, A.L.R.S. Universidade Federal Fluminense. Faculdade de Veterinária. Departamento de Patologia e Clínica Veterinaria; Brasil Fil: Cueto, Marcela Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina Fil: Gibbons, Alejandro Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina Fil: Fonseca, Jeferson Ferreira. Embrapa Caprinos e Ovinos. Núcleo Regional Sudeste; Brasil 2017-09-27T11:52:30Z 2017-09-27T11:52:30Z 2016 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:ar-repo/semantics/artículo http://hdl.handle.net/20.500.12123/1324 http://onlinelibrary.wiley.com/doi/10.1111/rda.12690/full 0936-6768 (Print) 1439-0531 (Online) DOI: 10.1111/rda.12690 eng info:eu-repo/semantics/restrictedAccess application/pdf Reproduction in domestic animals 51 (3) : 386–391. (June 2016) |
| spellingShingle | Producción Animal Caprinos Reproducción Animal Production Goats Reproduction Cueto, Marcela Isabel Gibbons, Alejandro Eduardo Balaro, Mario Felipe Alvarez Brandao, Felipe Zandonadi Maia, A.L.R.S. Souza-Fabjan, Joanna Maria Gonçalves Fonseca, Jeferson Ferreira Pre-Selection test to identify high responder donor goats |
| title | Pre-Selection test to identify high responder donor goats |
| title_full | Pre-Selection test to identify high responder donor goats |
| title_fullStr | Pre-Selection test to identify high responder donor goats |
| title_full_unstemmed | Pre-Selection test to identify high responder donor goats |
| title_short | Pre-Selection test to identify high responder donor goats |
| title_sort | pre selection test to identify high responder donor goats |
| topic | Producción Animal Caprinos Reproducción Animal Production Goats Reproduction |
| url | http://hdl.handle.net/20.500.12123/1324 http://onlinelibrary.wiley.com/doi/10.1111/rda.12690/full |
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