Unweaving the population structure and genetic diversity of Canadian shrub willow

Perennial shrub willow are increasingly being promoted in short-rotation coppice systems as biomass feedstocks, for phytoremediation applications, and for the diverse ecosystem services that can accrue. This renewed interest has led to widespread willow cultivation, particularly of non-native variet...

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Main Authors: Murphy, Emily K., Cappa, Eduardo Pablo, Soolanayakanahally, Raju Y., El-Kassaby, Yousry A., Parkin, Isobel A.P., Schroeder, William R., Mansfield, Shawn D.
Format: Artículo
Language:Inglés
Published: Springer Nature 2022
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/13183
https://www.nature.com/articles/s41598-022-20498-9
https://doi.org/10.1038/s41598-022-20498-9
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author Murphy, Emily K.
Cappa, Eduardo Pablo
Soolanayakanahally, Raju Y.
El-Kassaby, Yousry A.
Parkin, Isobel A.P.
Schroeder, William R.
Mansfield, Shawn D.
author_browse Cappa, Eduardo Pablo
El-Kassaby, Yousry A.
Mansfield, Shawn D.
Murphy, Emily K.
Parkin, Isobel A.P.
Schroeder, William R.
Soolanayakanahally, Raju Y.
author_facet Murphy, Emily K.
Cappa, Eduardo Pablo
Soolanayakanahally, Raju Y.
El-Kassaby, Yousry A.
Parkin, Isobel A.P.
Schroeder, William R.
Mansfield, Shawn D.
author_sort Murphy, Emily K.
collection INTA Digital
description Perennial shrub willow are increasingly being promoted in short-rotation coppice systems as biomass feedstocks, for phytoremediation applications, and for the diverse ecosystem services that can accrue. This renewed interest has led to widespread willow cultivation, particularly of non-native varieties. However, Canadian willow species have not been widely adopted and their inherent diversity has not yet been thoroughly investigated. In this study, 324 genotypes of Salix famelica and Salix eriocephala collected from 33 sites of origin were analyzed using 26,016 single nucleotide polymorphisms to reveal patterns of population structure and genetic diversity. Analyses by Bayesian methods and principal component analysis detected five main clusters that appeared to be largely shaped by geoclimatic variables including mean annual precipitation and the number of frost-free days. The overall observed (HO) and expected (HE) heterozygosity were 0.126 and 0.179, respectively. An analysis of molecular variance revealed that the highest genetic variation occurred within genotypes (69%), while 8% of the variation existed among clusters and 23% between genotypes within clusters. These findings provide new insights into the extent of genetic variation that exists within native shrub willow species which could be leveraged in pan-Canadian willow breeding programs.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
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publishDateRange 2022
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spelling INTA131832022-10-21T14:31:39Z Unweaving the population structure and genetic diversity of Canadian shrub willow Murphy, Emily K. Cappa, Eduardo Pablo Soolanayakanahally, Raju Y. El-Kassaby, Yousry A. Parkin, Isobel A.P. Schroeder, William R. Mansfield, Shawn D. Salix Plantas Leñosas Ornamentales Variación Genética Estructura de la Población Ornamental Woody Plants Genetic Variation Population Structure Sauce Arbustivo Perenne Sauce (salix) Willow Perennial shrub willow are increasingly being promoted in short-rotation coppice systems as biomass feedstocks, for phytoremediation applications, and for the diverse ecosystem services that can accrue. This renewed interest has led to widespread willow cultivation, particularly of non-native varieties. However, Canadian willow species have not been widely adopted and their inherent diversity has not yet been thoroughly investigated. In this study, 324 genotypes of Salix famelica and Salix eriocephala collected from 33 sites of origin were analyzed using 26,016 single nucleotide polymorphisms to reveal patterns of population structure and genetic diversity. Analyses by Bayesian methods and principal component analysis detected five main clusters that appeared to be largely shaped by geoclimatic variables including mean annual precipitation and the number of frost-free days. The overall observed (HO) and expected (HE) heterozygosity were 0.126 and 0.179, respectively. An analysis of molecular variance revealed that the highest genetic variation occurred within genotypes (69%), while 8% of the variation existed among clusters and 23% between genotypes within clusters. These findings provide new insights into the extent of genetic variation that exists within native shrub willow species which could be leveraged in pan-Canadian willow breeding programs. Fil: Murphy, Emily K. University of British Columbia. Faculty of Forestry. Department of Wood Science; Canadá Fil: Cappa, Eduardo Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soolanayakanahally, Raju Y. Agriculture and Agri-Food Canada. Indian Head Research Farm; Canadá. Agriculture and Agri-Food Canada. Saskatoon Research and Development Centre; Canadá. Fil: El-Kassaby, Yousry A. University of British Columbia. Faculty of Forestry. Department of Forest and Conservation Sciences; Canadá Fil: Parkin, Isobel A.P. Agriculture and Agri-Food Canada. Saskatoon Research and Development Centre; Canadá. Fil: Schroeder, William R. Agriculture and Agri-Food Canada. Indian Head Research Farm; Canadá Fil: Mansfield, Shawn D. University of British Columbia. Faculty of Forestry. Department of Wood Science; Canadá 2022-10-21T14:13:07Z 2022-10-21T14:13:07Z 2022-10-14 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13183 https://www.nature.com/articles/s41598-022-20498-9 2045-2322 https://doi.org/10.1038/s41598-022-20498-9 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Nature Scientific Reports 12 : Article number: 17254 (2022)
spellingShingle Salix
Plantas Leñosas Ornamentales
Variación Genética
Estructura de la Población
Ornamental Woody Plants
Genetic Variation
Population Structure
Sauce Arbustivo Perenne
Sauce (salix)
Willow
Murphy, Emily K.
Cappa, Eduardo Pablo
Soolanayakanahally, Raju Y.
El-Kassaby, Yousry A.
Parkin, Isobel A.P.
Schroeder, William R.
Mansfield, Shawn D.
Unweaving the population structure and genetic diversity of Canadian shrub willow
title Unweaving the population structure and genetic diversity of Canadian shrub willow
title_full Unweaving the population structure and genetic diversity of Canadian shrub willow
title_fullStr Unweaving the population structure and genetic diversity of Canadian shrub willow
title_full_unstemmed Unweaving the population structure and genetic diversity of Canadian shrub willow
title_short Unweaving the population structure and genetic diversity of Canadian shrub willow
title_sort unweaving the population structure and genetic diversity of canadian shrub willow
topic Salix
Plantas Leñosas Ornamentales
Variación Genética
Estructura de la Población
Ornamental Woody Plants
Genetic Variation
Population Structure
Sauce Arbustivo Perenne
Sauce (salix)
Willow
url http://hdl.handle.net/20.500.12123/13183
https://www.nature.com/articles/s41598-022-20498-9
https://doi.org/10.1038/s41598-022-20498-9
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