Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker
Apple valsa canker, caused by the fungus Valsa ceratosperma, is one of the most destructive diseases of this crop. Conventional fungicide treatments are not effective enough; therefore, it is necessary to develop alternatives for the control of this pathogen. The use of Trichoderma strains as a bioc...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/13082 https://link.springer.com/article/10.1007/s10658-022-02529-3 https://doi.org/10.1007/s10658-022-02529-3 |
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| author | Valetti, Lucio Bernanrdi Lima, Nelson Cazon, Luis Ignacio Crociara, Clara Sonia Ortega, Leandro Ismael Pastor, Silvina Estela |
| author_browse | Bernanrdi Lima, Nelson Cazon, Luis Ignacio Crociara, Clara Sonia Ortega, Leandro Ismael Pastor, Silvina Estela Valetti, Lucio |
| author_facet | Valetti, Lucio Bernanrdi Lima, Nelson Cazon, Luis Ignacio Crociara, Clara Sonia Ortega, Leandro Ismael Pastor, Silvina Estela |
| author_sort | Valetti, Lucio |
| collection | INTA Digital |
| description | Apple valsa canker, caused by the fungus Valsa ceratosperma, is one of the most destructive diseases of this crop. Conventional fungicide treatments are not effective enough; therefore, it is necessary to develop alternatives for the control of this pathogen. The use of Trichoderma strains as a biocontrol agent is a promising strategy for the environmentally friendly management of this disease. In this study, seven different isolates of Trichoderma spp. were obtained from the rhizospheric soil of healthy apple plants. The antagonistic capacity of the isolates against the pathogen V. ceratosperma was evaluated using the dual culture method and by the production of antibiotic non-volatile compounds. In the dual culture method, the interaction zone was observed by differential interference contrast microscopy and confocal laser scanning microscopy. The isolates RN-13, RN-33, and RN-34 inhibited more than 75% of mycelial growth, whereas RN-19, RN-16, and RN-15 inhibited growth by 65–72% with respect to the control. The mycoparasitic activity was evidenced by the coiling of hyphae around pathogen hyphae, appressorium-like structures, morphological deformations, and disintegration of mycelial walls. Apple shoots inoculated with Trichoderma isolates and then infected with V. ceratosperma showed the antagonist capacity of RN-16, RN-19, RN-15, and RN-18, which produced a significant decrease in the size of the lesion. Overall, the results showed the great potential of Trichoderma as a biocontrol agent against apple tree valsa canker. Further studies of this fungal interaction are required to design effective control strategies for the protection of apple tree orchards from this disease. |
| format | info:ar-repo/semantics/artículo |
| id | INTA13082 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA130822022-10-11T11:39:09Z Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker Valetti, Lucio Bernanrdi Lima, Nelson Cazon, Luis Ignacio Crociara, Clara Sonia Ortega, Leandro Ismael Pastor, Silvina Estela Antagonism Trichoderma Apples Biological Control Agents Antagonismo Valsa Manzana Agentes de Control Biológico Biocontrol Agents Canker Disease Mycoparasitism Valsa ceratosperma Apple valsa canker, caused by the fungus Valsa ceratosperma, is one of the most destructive diseases of this crop. Conventional fungicide treatments are not effective enough; therefore, it is necessary to develop alternatives for the control of this pathogen. The use of Trichoderma strains as a biocontrol agent is a promising strategy for the environmentally friendly management of this disease. In this study, seven different isolates of Trichoderma spp. were obtained from the rhizospheric soil of healthy apple plants. The antagonistic capacity of the isolates against the pathogen V. ceratosperma was evaluated using the dual culture method and by the production of antibiotic non-volatile compounds. In the dual culture method, the interaction zone was observed by differential interference contrast microscopy and confocal laser scanning microscopy. The isolates RN-13, RN-33, and RN-34 inhibited more than 75% of mycelial growth, whereas RN-19, RN-16, and RN-15 inhibited growth by 65–72% with respect to the control. The mycoparasitic activity was evidenced by the coiling of hyphae around pathogen hyphae, appressorium-like structures, morphological deformations, and disintegration of mycelial walls. Apple shoots inoculated with Trichoderma isolates and then infected with V. ceratosperma showed the antagonist capacity of RN-16, RN-19, RN-15, and RN-18, which produced a significant decrease in the size of the lesion. Overall, the results showed the great potential of Trichoderma as a biocontrol agent against apple tree valsa canker. Further studies of this fungal interaction are required to design effective control strategies for the protection of apple tree orchards from this disease. Instituto de Patología Vegetal Fil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Bernanrdi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Bernanrdi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Cazon, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Cazon, Luis Ignacio. Universidad Federal de Vicosa. Laboratorio de Epidemiología. Departamento de Fitopatología; Brasil Fil: Crociara, Clara Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Crociara, Clara Sonia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Ortega, Leandro Ismael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Pastor, Silvina Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina 2022-10-11T11:31:22Z 2022-10-11T11:31:22Z 2022-05-27 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13082 https://link.springer.com/article/10.1007/s10658-022-02529-3 0929-1873 1573-8469 (online) https://doi.org/10.1007/s10658-022-02529-3 eng info:eu-repograntAgreement/INTA/PNFRU-1105073/AR./Generación y desarrollo de tecnología para la detección, seguimiento, predicción, prevención y control de vectores, plagas emergentes y/o limitantes de la producción frutícola argentina. info:eu-repo/semantics/restrictedAccess application/pdf Springer European Journal of Plant Pathology 163 : 923–935 (2022) |
| spellingShingle | Antagonism Trichoderma Apples Biological Control Agents Antagonismo Valsa Manzana Agentes de Control Biológico Biocontrol Agents Canker Disease Mycoparasitism Valsa ceratosperma Valetti, Lucio Bernanrdi Lima, Nelson Cazon, Luis Ignacio Crociara, Clara Sonia Ortega, Leandro Ismael Pastor, Silvina Estela Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker |
| title | Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker |
| title_full | Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker |
| title_fullStr | Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker |
| title_full_unstemmed | Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker |
| title_short | Mycoparasitic Trichoderma isolates as a biocontrol agent against Valsa ceratosperma, the causal agent of apple valsa canker |
| title_sort | mycoparasitic trichoderma isolates as a biocontrol agent against valsa ceratosperma the causal agent of apple valsa canker |
| topic | Antagonism Trichoderma Apples Biological Control Agents Antagonismo Valsa Manzana Agentes de Control Biológico Biocontrol Agents Canker Disease Mycoparasitism Valsa ceratosperma |
| url | http://hdl.handle.net/20.500.12123/13082 https://link.springer.com/article/10.1007/s10658-022-02529-3 https://doi.org/10.1007/s10658-022-02529-3 |
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