New host record of Sclerotium rolfsii causing crown and root rot on Pseudogynoxis benthamii
Symptoms of a wilt disease were observed on 10 of 40 plants of Pseudogynoxis benthamii grown in Buenos Aires, Argentina, in March 2016. The aim of this study was to identify the causal agent of the disease. Five phenotypically identical fungal isolates were obtained from sclerotia that developed on...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Instituto de Biología, Universidad Nacional Autónoma de México
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/13072 http://revista.ib.unam.mx/index.php/bio/article/view/2187 https://doi.org/10.22201/ib.20078706e.2018.3.2187 |
| _version_ | 1855036871302709248 |
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| author | Rivera, Marta Carolina Wright, Eduardo Roberto Silvestro, Luciana Belén Steinglein, Sebastián Alberto Kato, Adriana Elena |
| author_browse | Kato, Adriana Elena Rivera, Marta Carolina Silvestro, Luciana Belén Steinglein, Sebastián Alberto Wright, Eduardo Roberto |
| author_facet | Rivera, Marta Carolina Wright, Eduardo Roberto Silvestro, Luciana Belén Steinglein, Sebastián Alberto Kato, Adriana Elena |
| author_sort | Rivera, Marta Carolina |
| collection | INTA Digital |
| description | Symptoms of a wilt disease were observed on 10 of 40 plants of Pseudogynoxis benthamii grown in Buenos Aires, Argentina, in March 2016. The aim of this study was to identify the causal agent of the disease. Five phenotypically identical fungal isolates were obtained from sclerotia that developed on the roots of wilted plants. One of them was inoculated onto healthy plants, and caused symptoms in 2 weeks. The pathogen was identified as Sclerotium rolfsii, on the basis of morphological characteristics. The nuclear ribosomal DNA internal transcribed spacer region of the isolate was PCR-amplified and sequenced. DNA analysis revealed a 99-100% similarity with S. rolfsii. This is the first report of S. rolfsii causing wilt on P. benthamii and the first pathogen reported on this plant species worldwide. |
| format | info:ar-repo/semantics/artículo |
| id | INTA13072 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Instituto de Biología, Universidad Nacional Autónoma de México |
| publisherStr | Instituto de Biología, Universidad Nacional Autónoma de México |
| record_format | dspace |
| spelling | INTA130722022-10-06T13:18:55Z New host record of Sclerotium rolfsii causing crown and root rot on Pseudogynoxis benthamii Rivera, Marta Carolina Wright, Eduardo Roberto Silvestro, Luciana Belén Steinglein, Sebastián Alberto Kato, Adriana Elena Wilts Inoculation Soil Morphological Features Soil Fungi Marchitez Inoculación Características Morfológicas Suelo Hongos del Suelo Corticium rolfsii Soilborne Fungi Sclerotium rolfsii Pseudogynoxis benthamii Symptoms of a wilt disease were observed on 10 of 40 plants of Pseudogynoxis benthamii grown in Buenos Aires, Argentina, in March 2016. The aim of this study was to identify the causal agent of the disease. Five phenotypically identical fungal isolates were obtained from sclerotia that developed on the roots of wilted plants. One of them was inoculated onto healthy plants, and caused symptoms in 2 weeks. The pathogen was identified as Sclerotium rolfsii, on the basis of morphological characteristics. The nuclear ribosomal DNA internal transcribed spacer region of the isolate was PCR-amplified and sequenced. DNA analysis revealed a 99-100% similarity with S. rolfsii. This is the first report of S. rolfsii causing wilt on P. benthamii and the first pathogen reported on this plant species worldwide. En marzo de 2016, de un total de 40 plantas de Pseudogynoxis benthamii cultivadas en Buenos Aires, Argentina, se observó marchitez en 10 de ellas. El objetivo de este trabajo fue identificar el agente causal de la enfermedad. Se obtuvieron 5 aislados fúngicos de características similares a partir de esclerocios desarrollados sobre las raíces, de los cuales se seleccionó 1, que luego de ser inoculado en plantas sanas, causó síntomas en un período de 2 semanas. El patógeno fue identificado como Sclerotium rolfsii sobre la base de sus características morfológicas. Se amplificó y secuenció la región de la espaciadora interna transcrita de ADN ribosomal del núcleo del aislado. El análisis de ADN mostró 99-100% de similitud para S. rolfsii. Este es el primer reporte de S. rolfsii como agente causal de marchitez en P. benthamii y el primer patógeno citado sobre esta especie en el mundo. Fil: Rivera, Marta Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; Argentina Fil: Wright, Eduardo Roberto. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; Argentina Fil: Silvestro, Luciana. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía. Laboratorio de Biología Funcional y Biotecnología (BIOLAB-INBIOTEC); Argentina Fil: Stenglein, Sebastian Alberto. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Kato, Adriana Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina 2022-10-06T12:58:27Z 2022-10-06T12:58:27Z 2018-03-13 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13072 http://revista.ib.unam.mx/index.php/bio/article/view/2187 2007-8706 https://doi.org/10.22201/ib.20078706e.2018.3.2187 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Instituto de Biología, Universidad Nacional Autónoma de México Revista Mexicana de Biodiversidad 89 (3) : 950-953 (2018) |
| spellingShingle | Wilts Inoculation Soil Morphological Features Soil Fungi Marchitez Inoculación Características Morfológicas Suelo Hongos del Suelo Corticium rolfsii Soilborne Fungi Sclerotium rolfsii Pseudogynoxis benthamii Rivera, Marta Carolina Wright, Eduardo Roberto Silvestro, Luciana Belén Steinglein, Sebastián Alberto Kato, Adriana Elena New host record of Sclerotium rolfsii causing crown and root rot on Pseudogynoxis benthamii |
| title | New host record of Sclerotium rolfsii causing crown and root rot on Pseudogynoxis benthamii |
| title_full | New host record of Sclerotium rolfsii causing crown and root rot on Pseudogynoxis benthamii |
| title_fullStr | New host record of Sclerotium rolfsii causing crown and root rot on Pseudogynoxis benthamii |
| title_full_unstemmed | New host record of Sclerotium rolfsii causing crown and root rot on Pseudogynoxis benthamii |
| title_short | New host record of Sclerotium rolfsii causing crown and root rot on Pseudogynoxis benthamii |
| title_sort | new host record of sclerotium rolfsii causing crown and root rot on pseudogynoxis benthamii |
| topic | Wilts Inoculation Soil Morphological Features Soil Fungi Marchitez Inoculación Características Morfológicas Suelo Hongos del Suelo Corticium rolfsii Soilborne Fungi Sclerotium rolfsii Pseudogynoxis benthamii |
| url | http://hdl.handle.net/20.500.12123/13072 http://revista.ib.unam.mx/index.php/bio/article/view/2187 https://doi.org/10.22201/ib.20078706e.2018.3.2187 |
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