Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina
Ramularia leaf spot (RLS) caused by the fungus Ramularia collo-cygni (Rcc) has become a threat to barley production in Argentina. All barley varieties are susceptible to RLS; thus, disease management relies on fungicides like quinone-outside inhibitors (QoIs), demethylation inhibitors (DMIs) and suc...
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/12974 https://link.springer.com/article/10.1007/s41348-022-00645-0 https://doi.org/10.1007/s41348-022-00645-0 |
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| author | Erreguerena, Ignacio Antonio Havis, Neil David Heick, Thies Marten Gorniak, Kalina Quiroz, Facundo Jose Carmona, Marcelo Anibal |
| author_browse | Carmona, Marcelo Anibal Erreguerena, Ignacio Antonio Gorniak, Kalina Havis, Neil David Heick, Thies Marten Quiroz, Facundo Jose |
| author_facet | Erreguerena, Ignacio Antonio Havis, Neil David Heick, Thies Marten Gorniak, Kalina Quiroz, Facundo Jose Carmona, Marcelo Anibal |
| author_sort | Erreguerena, Ignacio Antonio |
| collection | INTA Digital |
| description | Ramularia leaf spot (RLS) caused by the fungus Ramularia collo-cygni (Rcc) has become a threat to barley production in Argentina. All barley varieties are susceptible to RLS; thus, disease management relies on fungicides like quinone-outside inhibitors (QoIs), demethylation inhibitors (DMIs) and succinate dehydrogenase inhibitors (SDHIs). Europe reported Rcc resistance to fungicides associated with mutations in the fungicide's target genes. The aim of this work was to assess the impact of mutations on sensitivity levels in isolates of Rcc to QoIs, DMIs, SDHIs and a multi-site fungicide used for RLS control in Argentina and others countries. Twenty-one Rcc isolates were collected in the Buenos Aires province in the years 2012, 2015 and 2017 and tested against azoxystrobin (QoI), epoxiconazole and prothioconazole-desthio (DMIs), isopyrazam and fluxapyroxad (SDHIs) and chlorothalonil. For EC50 determination, a sensitivity assay was performed and cytb, cyp51 and sdh genes were sequenced for mutation analyses. All isolates showed G143A mutation that confers total resistance to QoIs (EC50 > 1 mg L−1). Three mutations in the target genes of DMIs (Y403C/S, G404D) were observed. Isolates with both amino-exchanges showed an increase in the EC50 towards DMIs. Mutations B-H266L and C-N87S in the target genes of SDHIs were detected, and both were related to a sensitivity reduction. Mutations occurrence tended to increase from 2012 to 2017 along with higher EC50 values. We found six haplotypes showing that Rcc is potentially adapting to fungicides in Argentina. Efforts must focus on new active ingredients and resistant varieties for a less fungicide-dependent agriculture based on IPM strategies. |
| format | Artículo |
| id | INTA12974 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA129742022-09-27T10:43:01Z Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina Erreguerena, Ignacio Antonio Havis, Neil David Heick, Thies Marten Gorniak, Kalina Quiroz, Facundo Jose Carmona, Marcelo Anibal Cebada Boletus edulis Mutación Fungicidas Barley Mutation Fungicides Ramularia Hordeum vulgare Argentina Ramularia leaf spot (RLS) caused by the fungus Ramularia collo-cygni (Rcc) has become a threat to barley production in Argentina. All barley varieties are susceptible to RLS; thus, disease management relies on fungicides like quinone-outside inhibitors (QoIs), demethylation inhibitors (DMIs) and succinate dehydrogenase inhibitors (SDHIs). Europe reported Rcc resistance to fungicides associated with mutations in the fungicide's target genes. The aim of this work was to assess the impact of mutations on sensitivity levels in isolates of Rcc to QoIs, DMIs, SDHIs and a multi-site fungicide used for RLS control in Argentina and others countries. Twenty-one Rcc isolates were collected in the Buenos Aires province in the years 2012, 2015 and 2017 and tested against azoxystrobin (QoI), epoxiconazole and prothioconazole-desthio (DMIs), isopyrazam and fluxapyroxad (SDHIs) and chlorothalonil. For EC50 determination, a sensitivity assay was performed and cytb, cyp51 and sdh genes were sequenced for mutation analyses. All isolates showed G143A mutation that confers total resistance to QoIs (EC50 > 1 mg L−1). Three mutations in the target genes of DMIs (Y403C/S, G404D) were observed. Isolates with both amino-exchanges showed an increase in the EC50 towards DMIs. Mutations B-H266L and C-N87S in the target genes of SDHIs were detected, and both were related to a sensitivity reduction. Mutations occurrence tended to increase from 2012 to 2017 along with higher EC50 values. We found six haplotypes showing that Rcc is potentially adapting to fungicides in Argentina. Efforts must focus on new active ingredients and resistant varieties for a less fungicide-dependent agriculture based on IPM strategies. EEA Manfredi Fil: Erreguerena, Ignacio Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Havis, Neil David. Scotland’s Rural College. Crop and Soil Research Group; Reino Unido Fil: Heick, Thies Marten. Aarhus University. Department of Agroecology; Dinamarca Fil: Gorniak, Kalina. Scotland’s Rural College. Crop and Soil Research Group; Reino Unido Fil: Quiroz, Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía. Catedra de Fitopatología; Argentina 2022-09-27T10:16:40Z 2022-09-27T10:16:40Z 2022-06-23 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12974 https://link.springer.com/article/10.1007/s41348-022-00645-0 1861-3837 https://doi.org/10.1007/s41348-022-00645-0 eng info:eu-repograntAgreement/INTA/PNCYO-1127034/AR./Evaluación y desarrollo de sistemas de manejo integrado de las plagas en cultivos de cereales y oleaginosas. info:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola. info:eu-repo/semantics/restrictedAccess application/pdf Springer Journal of Plant Diseases and Protection 129 : 1-11 (2022) |
| spellingShingle | Cebada Boletus edulis Mutación Fungicidas Barley Mutation Fungicides Ramularia Hordeum vulgare Argentina Erreguerena, Ignacio Antonio Havis, Neil David Heick, Thies Marten Gorniak, Kalina Quiroz, Facundo Jose Carmona, Marcelo Anibal Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina |
| title | Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina |
| title_full | Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina |
| title_fullStr | Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina |
| title_full_unstemmed | Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina |
| title_short | Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina |
| title_sort | characterization of dmi qoi and sdhi fungicides sensitivity of ramularia collo cygni isolates in argentina |
| topic | Cebada Boletus edulis Mutación Fungicidas Barley Mutation Fungicides Ramularia Hordeum vulgare Argentina |
| url | http://hdl.handle.net/20.500.12123/12974 https://link.springer.com/article/10.1007/s41348-022-00645-0 https://doi.org/10.1007/s41348-022-00645-0 |
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