Evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti–Neospora caninum antibodies in cattle
Neospora caninum is a protozoan parasite that causes abortion and important economic losses in cattle worldwide. There are no treatments or vaccines available; disease control is based on diagnosis and herd management strategies. We developed, validated, and evaluated under field conditions a compet...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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American Association of Veterinary Laboratory Diagnosticians (AAVLD)
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/12900 https://journals.sagepub.com/doi/10.1177/1040638720916711 https://doi.org/10.1177/1040638720916711 |
| _version_ | 1855036841006202880 |
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| author | Novoa, María Belén Valentini, Beatriz Susana Torioni, Susana Marta Primo, María Evangelina Echaide, Ignacio Eduardo |
| author_browse | Echaide, Ignacio Eduardo Novoa, María Belén Primo, María Evangelina Torioni, Susana Marta Valentini, Beatriz Susana |
| author_facet | Novoa, María Belén Valentini, Beatriz Susana Torioni, Susana Marta Primo, María Evangelina Echaide, Ignacio Eduardo |
| author_sort | Novoa, María Belén |
| collection | INTA Digital |
| description | Neospora caninum is a protozoan parasite that causes abortion and important economic losses in cattle worldwide. There are no treatments or vaccines available; disease control is based on diagnosis and herd management strategies. We developed, validated, and evaluated under field conditions a competitive inhibition ELISA based on the truncated SAG1 protein (tSAG1), expressed in Escherichia coli, and the RafNeo5 monoclonal antibody (ciELISAtSAG1). A criterion based on the 3-y sequential serologic analysis of 230 dairy cows by IFAT was used as the gold standard. The assay was validated using 860 serum samples from cows that were consistently positive or negative by IFAT throughout the study period. ciELISAtSAG1 was then used to evaluate the prevalence of neosporosis in 16 beef cow herds (22 samples per herd, 352 total samples). The results were compared with those from IFAT and a commercial cELISA (cELISAVMRD). The ciELISAtSAG1 cutoff was ≥ 29%I, with a diagnostic sensitivity of 98.7% (95% CI = 96.8–99.7%) and a diagnostic specificity of 97.9% (95% CI = 96.4–99.0%). Concordance among IFAT, cELISAVMRD, and ciELISAtSAG1 was 90.3%. The agreement (κ) between ciELISAtSAG1 and the other 2 tests was ≥ 0.81. The overall prevalence of neosporosis in the 16 beef herds was 30% (range: 5–60%). The ciELISAtSAG1 could be useful for large-scale detection of anti–N. caninum antibodies in cattle and seroepidemiologic investigations, given its appropriate sensitivity and specificity, and the simplicity of production. |
| format | info:ar-repo/semantics/artículo |
| id | INTA12900 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | American Association of Veterinary Laboratory Diagnosticians (AAVLD) |
| publisherStr | American Association of Veterinary Laboratory Diagnosticians (AAVLD) |
| record_format | dspace |
| spelling | INTA129002022-09-16T11:59:04Z Evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti–Neospora caninum antibodies in cattle Novoa, María Belén Valentini, Beatriz Susana Torioni, Susana Marta Primo, María Evangelina Echaide, Ignacio Eduardo Cattle Competitive ELISA Diagnosis Recombinant Proteins Serology Ganado Bovino ELISA Competitivo Diagnóstico Proteínas Recombinantes Serología Prevalence Predominio SAG1 Neospora caninum is a protozoan parasite that causes abortion and important economic losses in cattle worldwide. There are no treatments or vaccines available; disease control is based on diagnosis and herd management strategies. We developed, validated, and evaluated under field conditions a competitive inhibition ELISA based on the truncated SAG1 protein (tSAG1), expressed in Escherichia coli, and the RafNeo5 monoclonal antibody (ciELISAtSAG1). A criterion based on the 3-y sequential serologic analysis of 230 dairy cows by IFAT was used as the gold standard. The assay was validated using 860 serum samples from cows that were consistently positive or negative by IFAT throughout the study period. ciELISAtSAG1 was then used to evaluate the prevalence of neosporosis in 16 beef cow herds (22 samples per herd, 352 total samples). The results were compared with those from IFAT and a commercial cELISA (cELISAVMRD). The ciELISAtSAG1 cutoff was ≥ 29%I, with a diagnostic sensitivity of 98.7% (95% CI = 96.8–99.7%) and a diagnostic specificity of 97.9% (95% CI = 96.4–99.0%). Concordance among IFAT, cELISAVMRD, and ciELISAtSAG1 was 90.3%. The agreement (κ) between ciELISAtSAG1 and the other 2 tests was ≥ 0.81. The overall prevalence of neosporosis in the 16 beef herds was 30% (range: 5–60%). The ciELISAtSAG1 could be useful for large-scale detection of anti–N. caninum antibodies in cattle and seroepidemiologic investigations, given its appropriate sensitivity and specificity, and the simplicity of production. EEA Rafaela Fil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Valentini, Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Torioni, Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Primo, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Echaide, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina 2022-09-16T11:46:07Z 2022-09-16T11:46:07Z 2020-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12900 https://journals.sagepub.com/doi/10.1177/1040638720916711 1040-6387 1943-4936 (online) https://doi.org/10.1177/1040638720916711 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Association of Veterinary Laboratory Diagnosticians (AAVLD) Journal of Veterinary Diagnostic Investigation 32 (3) : 401-408 (May 2020) |
| spellingShingle | Cattle Competitive ELISA Diagnosis Recombinant Proteins Serology Ganado Bovino ELISA Competitivo Diagnóstico Proteínas Recombinantes Serología Prevalence Predominio SAG1 Novoa, María Belén Valentini, Beatriz Susana Torioni, Susana Marta Primo, María Evangelina Echaide, Ignacio Eduardo Evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti–Neospora caninum antibodies in cattle |
| title | Evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti–Neospora caninum antibodies in cattle |
| title_full | Evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti–Neospora caninum antibodies in cattle |
| title_fullStr | Evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti–Neospora caninum antibodies in cattle |
| title_full_unstemmed | Evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti–Neospora caninum antibodies in cattle |
| title_short | Evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti–Neospora caninum antibodies in cattle |
| title_sort | evaluation of a competitive inhibition elisa based on the recombinant protein tsag1 to detect anti neospora caninum antibodies in cattle |
| topic | Cattle Competitive ELISA Diagnosis Recombinant Proteins Serology Ganado Bovino ELISA Competitivo Diagnóstico Proteínas Recombinantes Serología Prevalence Predominio SAG1 |
| url | http://hdl.handle.net/20.500.12123/12900 https://journals.sagepub.com/doi/10.1177/1040638720916711 https://doi.org/10.1177/1040638720916711 |
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