Analysis of sequence variations in the GABA gated chloride gene associated with resistance to fipronil in Rhipicephalus (Boophilus) microplus ticks from Argentina
This study aimed to evaluate the sequence variations of the GABA-Cl gene of Rhipicephalus (Boophilus) microplus ticks from Argentina with different resistance levels to fipronil. Genomic DNA was obtained from engorged females (n = 50) of fipronil-susceptible and resistant tick field populations from...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/12884 https://www.sciencedirect.com/science/article/pii/S1877959X22001327 https://doi.org/10.1016/j.ttbdis.2022.102030 |
| _version_ | 1855485059248685056 |
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| author | Sarli, Macarena Torrents, Jorgelina Morel, Nicolas Nava, Santiago |
| author_browse | Morel, Nicolas Nava, Santiago Sarli, Macarena Torrents, Jorgelina |
| author_facet | Sarli, Macarena Torrents, Jorgelina Morel, Nicolas Nava, Santiago |
| author_sort | Sarli, Macarena |
| collection | INTA Digital |
| description | This study aimed to evaluate the sequence variations of the GABA-Cl gene of Rhipicephalus (Boophilus) microplus ticks from Argentina with different resistance levels to fipronil. Genomic DNA was obtained from engorged females (n = 50) of fipronil-susceptible and resistant tick field populations from five localities in northern Argentina. Tick populations were tested for fipronil resistance by in vitro larval packet test. DNA encoding amino acid residues 264–360 of the GABA-Cl was amplified by PCR. PCR products were sequenced and the translated amino acid sequences were aligned and compared among each other and with sequences of the GABA-Cl belonging to the Brazilian and Uruguayan reference strains fipronil-susceptible (Mozo) and resistant (RFSan and Juarez). All the GABA-Cl amino acid sequences obtained from the R. microplus specimens from Argentina were identical, regardless of the fipronil resistance-susceptibility status of each population. Mutations present in GABA-Cl sequence of RFSan and Juarez strains were not present in any of the populations from Argentina analyzed. These results indicate the failure of this gene as a molecular marker of resistance to fipronil, at least in the R. microplus populations from northern Argentina. |
| format | Artículo |
| id | INTA12884 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA128842022-09-15T10:39:15Z Analysis of sequence variations in the GABA gated chloride gene associated with resistance to fipronil in Rhipicephalus (Boophilus) microplus ticks from Argentina Sarli, Macarena Torrents, Jorgelina Morel, Nicolas Nava, Santiago Rhipicephalus Boophilus microplus Gamma-aminobutyric Acid Fipronil Insecticide Resistance Ácido Gamma-aminobutírico Resistencia a los Insecticidas Argentina GABA This study aimed to evaluate the sequence variations of the GABA-Cl gene of Rhipicephalus (Boophilus) microplus ticks from Argentina with different resistance levels to fipronil. Genomic DNA was obtained from engorged females (n = 50) of fipronil-susceptible and resistant tick field populations from five localities in northern Argentina. Tick populations were tested for fipronil resistance by in vitro larval packet test. DNA encoding amino acid residues 264–360 of the GABA-Cl was amplified by PCR. PCR products were sequenced and the translated amino acid sequences were aligned and compared among each other and with sequences of the GABA-Cl belonging to the Brazilian and Uruguayan reference strains fipronil-susceptible (Mozo) and resistant (RFSan and Juarez). All the GABA-Cl amino acid sequences obtained from the R. microplus specimens from Argentina were identical, regardless of the fipronil resistance-susceptibility status of each population. Mutations present in GABA-Cl sequence of RFSan and Juarez strains were not present in any of the populations from Argentina analyzed. These results indicate the failure of this gene as a molecular marker of resistance to fipronil, at least in the R. microplus populations from northern Argentina. EEA Rafaela Fil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Torrents, Jorgelina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Morel, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina 2022-09-15T10:29:43Z 2022-09-15T10:29:43Z 2022-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12884 https://www.sciencedirect.com/science/article/pii/S1877959X22001327 Macarena Sarli, Jorgelina Torrents, Nicolás Morel, Santiago Nava, Analysis of sequence variations in the GABA gated chloride gene associated with resistance to fipronil in Rhipicephalus (Boophilus) microplus ticks from Argentina, Ticks and Tick-borne Diseases, Volume 13, Issue 6, 2022, 102030. 1877-959X https://doi.org/10.1016/j.ttbdis.2022.102030 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Ticks and Tick-borne Diseases 13 (6) : 102030 (November 2022) |
| spellingShingle | Rhipicephalus Boophilus microplus Gamma-aminobutyric Acid Fipronil Insecticide Resistance Ácido Gamma-aminobutírico Resistencia a los Insecticidas Argentina GABA Sarli, Macarena Torrents, Jorgelina Morel, Nicolas Nava, Santiago Analysis of sequence variations in the GABA gated chloride gene associated with resistance to fipronil in Rhipicephalus (Boophilus) microplus ticks from Argentina |
| title | Analysis of sequence variations in the GABA gated chloride gene associated with resistance to fipronil in Rhipicephalus (Boophilus) microplus ticks from Argentina |
| title_full | Analysis of sequence variations in the GABA gated chloride gene associated with resistance to fipronil in Rhipicephalus (Boophilus) microplus ticks from Argentina |
| title_fullStr | Analysis of sequence variations in the GABA gated chloride gene associated with resistance to fipronil in Rhipicephalus (Boophilus) microplus ticks from Argentina |
| title_full_unstemmed | Analysis of sequence variations in the GABA gated chloride gene associated with resistance to fipronil in Rhipicephalus (Boophilus) microplus ticks from Argentina |
| title_short | Analysis of sequence variations in the GABA gated chloride gene associated with resistance to fipronil in Rhipicephalus (Boophilus) microplus ticks from Argentina |
| title_sort | analysis of sequence variations in the gaba gated chloride gene associated with resistance to fipronil in rhipicephalus boophilus microplus ticks from argentina |
| topic | Rhipicephalus Boophilus microplus Gamma-aminobutyric Acid Fipronil Insecticide Resistance Ácido Gamma-aminobutírico Resistencia a los Insecticidas Argentina GABA |
| url | http://hdl.handle.net/20.500.12123/12884 https://www.sciencedirect.com/science/article/pii/S1877959X22001327 https://doi.org/10.1016/j.ttbdis.2022.102030 |
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