Chlamydia pecorum : fetal and placental lesions in sporadic caprine abortion
Chlamydial abortion in small ruminants is usually associated with Chlamydia abortus infection. Although Chlamydia pecorum has been detected in aborted ruminants and epidemiological data suggests that C. pecorum is abortigenic in these species, published descriptions of lesions in fetuses are lacking...
| Autores principales: | , , , , , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1285 http://journals.sagepub.com/doi/pdf/10.1177/1040638715625729 https://doi.org/10.1177/1040638715625729 |
| _version_ | 1855034778913341440 |
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| author | Giannitti, Federico Anderson, Mark Miller, Myrna Rowe, Joan Sverlow, Karen Vasquez, Marce Canton, German Jose |
| author_browse | Anderson, Mark Canton, German Jose Giannitti, Federico Miller, Myrna Rowe, Joan Sverlow, Karen Vasquez, Marce |
| author_facet | Giannitti, Federico Anderson, Mark Miller, Myrna Rowe, Joan Sverlow, Karen Vasquez, Marce Canton, German Jose |
| author_sort | Giannitti, Federico |
| collection | INTA Digital |
| description | Chlamydial abortion in small ruminants is usually associated with Chlamydia abortus infection. Although Chlamydia pecorum has been detected in aborted ruminants and epidemiological data suggests that C. pecorum is abortigenic in these species, published descriptions of lesions in fetuses are lacking. This work describes fetoplacental lesions in a caprine abortion with C. pecorum infection, and further supports the abortigenic role of C. pecorum in ruminants. A 16-month-old Boer goat aborted twin fetuses at ~130 days of gestation. Both fetuses (A and B) and the placenta of fetus A were submitted for postmortem examination and diagnostic workup. At autopsy, the fetuses had moderate anasarca, intermuscular edema in the hindquarters (A), and brachygnathia and palatoschisis (B). In the placenta, the cotyledons were covered by yellow fibrinosuppurative exudate that extended into the adjacent intercotyledonary areas. Histologically, there was severe suppurative and necrotizing placentitis with vasculitis (arteriolitis) and thrombosis, multifocal lymphohistiocytic and neutrophilic hepatitis (A), and fibrinosuppurative enteritis in both fetuses. Chlamydia antigen was detected in the placenta by the direct fluorescent antibody test and in fetal intestines by immunohistochemistry. Nested polymerase chain reaction of DNA extracted from formalin-fixed, paraffin-embedded sections of placenta and intestine amplified 400 bp of the Chlamydia 16S rRNA gene that was sequenced and found to be 99% identical to C. pecorum by BLAST analysis. Other known abortigenic infectious
agents were ruled out by specific testing. It is concluded that C. pecorum infection is associated with fetoplacental lesions and
sporadic abortion in goats. |
| format | info:eu-repo/semantics/article |
| id | INTA1285 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA12852019-01-28T12:04:02Z Chlamydia pecorum : fetal and placental lesions in sporadic caprine abortion Giannitti, Federico Anderson, Mark Miller, Myrna Rowe, Joan Sverlow, Karen Vasquez, Marce Canton, German Jose Caprinos Enfermedades de los Animales Aborto Chlamydia Goats Animal Diseases Abortion Chlamydial abortion in small ruminants is usually associated with Chlamydia abortus infection. Although Chlamydia pecorum has been detected in aborted ruminants and epidemiological data suggests that C. pecorum is abortigenic in these species, published descriptions of lesions in fetuses are lacking. This work describes fetoplacental lesions in a caprine abortion with C. pecorum infection, and further supports the abortigenic role of C. pecorum in ruminants. A 16-month-old Boer goat aborted twin fetuses at ~130 days of gestation. Both fetuses (A and B) and the placenta of fetus A were submitted for postmortem examination and diagnostic workup. At autopsy, the fetuses had moderate anasarca, intermuscular edema in the hindquarters (A), and brachygnathia and palatoschisis (B). In the placenta, the cotyledons were covered by yellow fibrinosuppurative exudate that extended into the adjacent intercotyledonary areas. Histologically, there was severe suppurative and necrotizing placentitis with vasculitis (arteriolitis) and thrombosis, multifocal lymphohistiocytic and neutrophilic hepatitis (A), and fibrinosuppurative enteritis in both fetuses. Chlamydia antigen was detected in the placenta by the direct fluorescent antibody test and in fetal intestines by immunohistochemistry. Nested polymerase chain reaction of DNA extracted from formalin-fixed, paraffin-embedded sections of placenta and intestine amplified 400 bp of the Chlamydia 16S rRNA gene that was sequenced and found to be 99% identical to C. pecorum by BLAST analysis. Other known abortigenic infectious agents were ruled out by specific testing. It is concluded that C. pecorum infection is associated with fetoplacental lesions and sporadic abortion in goats. EEA Balcarce Fil: Giannitti, Federico. University of Minnesota. College of Veterinary Medicine. Veterinary Population Medicine Department. Veterinary Diagnostic Laboratory; Estados Unidos. Instituto Nacional de Investigaciones Agropecuarias. La Estanzuela, Colonia;Uruguay Fil: Anderson, Mark University of California. School of Veterinary Medicine. California Animal Health and Food Safety Laboratory System; Estados Unidos Fil: Miller, Myrna. University of Wyoming. Wyoming State Veterinary Laboratory; Estados Unidos Fil: Rowe, Joan. University of California. School of Veterinary Medicine; Estados Unidos Fil: Sverlow, Karen. University of Minnesota. College of Veterinary Medicine. Veterinary Population Medicine Department. Veterinary Diagnostic Laboratory; Estados Unidos Fil: Vasquez, Marce. University of Wyoming. Wyoming State Veterinary Laboratory; Estados Unidos Fil: Canton, German Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina 2017-09-21T18:09:50Z 2017-09-21T18:09:50Z 2016 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:ar-repo/semantics/artículo http://hdl.handle.net/20.500.12123/1285 http://journals.sagepub.com/doi/pdf/10.1177/1040638715625729 1040-6387 (Print) 1943-4936 (Online) https://doi.org/10.1177/1040638715625729 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of veterinary diagnostic investigation 28 (2) : 184–189. (2016) |
| spellingShingle | Caprinos Enfermedades de los Animales Aborto Chlamydia Goats Animal Diseases Abortion Giannitti, Federico Anderson, Mark Miller, Myrna Rowe, Joan Sverlow, Karen Vasquez, Marce Canton, German Jose Chlamydia pecorum : fetal and placental lesions in sporadic caprine abortion |
| title | Chlamydia pecorum : fetal and placental lesions in sporadic caprine abortion |
| title_full | Chlamydia pecorum : fetal and placental lesions in sporadic caprine abortion |
| title_fullStr | Chlamydia pecorum : fetal and placental lesions in sporadic caprine abortion |
| title_full_unstemmed | Chlamydia pecorum : fetal and placental lesions in sporadic caprine abortion |
| title_short | Chlamydia pecorum : fetal and placental lesions in sporadic caprine abortion |
| title_sort | chlamydia pecorum fetal and placental lesions in sporadic caprine abortion |
| topic | Caprinos Enfermedades de los Animales Aborto Chlamydia Goats Animal Diseases Abortion |
| url | http://hdl.handle.net/20.500.12123/1285 http://journals.sagepub.com/doi/pdf/10.1177/1040638715625729 https://doi.org/10.1177/1040638715625729 |
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