In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses
Equine salmonellosis is caused by several Salmonella serotypes, including Salmonella Newport, which cause enterocolitis and diarrhea. Treatment usually includes the administration of antibiotics. However, since multidrug-resistant Salmonella is commonly detected, alternative options to control the p...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/12744 https://www.sciencedirect.com/science/article/abs/pii/S0737080621002872 https://doi.org/10.1016/j.jevs.2021.103657 |
| _version_ | 1855485039305818112 |
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| author | Bustos, Carla Paola Leiva, Carlos Leónidas Gambarotta, Mariana Guida, Nora Chacana, Pablo |
| author_browse | Bustos, Carla Paola Chacana, Pablo Gambarotta, Mariana Guida, Nora Leiva, Carlos Leónidas |
| author_facet | Bustos, Carla Paola Leiva, Carlos Leónidas Gambarotta, Mariana Guida, Nora Chacana, Pablo |
| author_sort | Bustos, Carla Paola |
| collection | INTA Digital |
| description | Equine salmonellosis is caused by several Salmonella serotypes, including Salmonella Newport, which cause enterocolitis and diarrhea. Treatment usually includes the administration of antibiotics. However, since multidrug-resistant Salmonella is commonly detected, alternative options to control the pathogen are needed. One of these options is the use of specific egg yolk antibodies (IgY) for passive immunotherapy. Thus, the aim of our work was to produce IgY antibodies against an equine S. Newport strain and assess their in vitro inhibitory activity. To this end, laying hens were immunized with an inactivated S. Newport strain by using either Freund's or Montanide adjuvant and egg yolk extracts were obtained. The levels of specific IgY antibodies against Salmonella in sera and egg extracts were determined by dot-blot and microagglutination. Besides, the IgY extracts were characterized by total protein analysis, SDS-PAGE, Western Blot, and inhibition of bacterial motility. IgY extracts showed high purity (87.7 to 91.8 %), high microagglutination titers, and the ability to inhibit the motility of the bacterium. The results using Montanide were similar to those using the traditional Freund's adjuvant. Thus, Montanide may also be a good adjuvant to produce IgY. IgY-technology represents a potential tool for the control of salmonellosis in horses. |
| format | Artículo |
| id | INTA12744 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA127442022-08-31T16:25:41Z In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses Bustos, Carla Paola Leiva, Carlos Leónidas Gambarotta, Mariana Guida, Nora Chacana, Pablo Salmonella Caballos Anticuerpos Yema de Huevo Horses Antibodies Egg Yolk In Vitro Salmonella newport Equine salmonellosis is caused by several Salmonella serotypes, including Salmonella Newport, which cause enterocolitis and diarrhea. Treatment usually includes the administration of antibiotics. However, since multidrug-resistant Salmonella is commonly detected, alternative options to control the pathogen are needed. One of these options is the use of specific egg yolk antibodies (IgY) for passive immunotherapy. Thus, the aim of our work was to produce IgY antibodies against an equine S. Newport strain and assess their in vitro inhibitory activity. To this end, laying hens were immunized with an inactivated S. Newport strain by using either Freund's or Montanide adjuvant and egg yolk extracts were obtained. The levels of specific IgY antibodies against Salmonella in sera and egg extracts were determined by dot-blot and microagglutination. Besides, the IgY extracts were characterized by total protein analysis, SDS-PAGE, Western Blot, and inhibition of bacterial motility. IgY extracts showed high purity (87.7 to 91.8 %), high microagglutination titers, and the ability to inhibit the motility of the bacterium. The results using Montanide were similar to those using the traditional Freund's adjuvant. Thus, Montanide may also be a good adjuvant to produce IgY. IgY-technology represents a potential tool for the control of salmonellosis in horses. Instituto de Patobiología Fil: Bustos, Carla Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Bustos, Carla Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bustos, Carla Paola. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Enfermedades Infecciosas; Argentina Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gambarotta, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Bioestadística; Argentina Fil: Guida, Nora. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Enfermedades Infecciosas; Argentina Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2022-08-31T16:19:05Z 2022-08-31T16:19:05Z 2021-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12744 https://www.sciencedirect.com/science/article/abs/pii/S0737080621002872 0737-0806 https://doi.org/10.1016/j.jevs.2021.103657 eng info:eu-repograntAgreement/INTA/2019-PpAT-2.3.1-S.ANIM-001/2019-PpAT-2.3.1-S.ANIM-001/AR./Programa por Área Temática Salud Animal info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Journal of Equine Veterinary Science 103 : 103657 (Agosto 2021) |
| spellingShingle | Salmonella Caballos Anticuerpos Yema de Huevo Horses Antibodies Egg Yolk In Vitro Salmonella newport Bustos, Carla Paola Leiva, Carlos Leónidas Gambarotta, Mariana Guida, Nora Chacana, Pablo In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses |
| title | In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses |
| title_full | In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses |
| title_fullStr | In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses |
| title_full_unstemmed | In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses |
| title_short | In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses |
| title_sort | in vitro inhibitory activity of igy antibodies against salmonella ser newport isolated from horses |
| topic | Salmonella Caballos Anticuerpos Yema de Huevo Horses Antibodies Egg Yolk In Vitro Salmonella newport |
| url | http://hdl.handle.net/20.500.12123/12744 https://www.sciencedirect.com/science/article/abs/pii/S0737080621002872 https://doi.org/10.1016/j.jevs.2021.103657 |
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