Next-generation technologies and systems biology for the design of novel vaccines against apicomplexan parasites

Parasites of the phylum Apicomplexa are the causative agents of important diseases such as malaria, toxoplasmosis or cryptosporidiosis in humans, and babesiosis and coccidiosis in animals. Whereas the first human recombinant vaccine against malaria has been approved and recently recommended for wide...

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Autores principales: Tomazic, Mariela Luján, Marugán-Hernandez, Virginia, Rodriguez, Anabel Elisa
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: Frontiers Media 2022
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/12729
https://www.frontiersin.org/articles/10.3389/fvets.2021.800361/full
https://doi.org/10.3389/fvets.2021.800361
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author Tomazic, Mariela Luján
Marugán-Hernandez, Virginia
Rodriguez, Anabel Elisa
author_browse Marugán-Hernandez, Virginia
Rodriguez, Anabel Elisa
Tomazic, Mariela Luján
author_facet Tomazic, Mariela Luján
Marugán-Hernandez, Virginia
Rodriguez, Anabel Elisa
author_sort Tomazic, Mariela Luján
collection INTA Digital
description Parasites of the phylum Apicomplexa are the causative agents of important diseases such as malaria, toxoplasmosis or cryptosporidiosis in humans, and babesiosis and coccidiosis in animals. Whereas the first human recombinant vaccine against malaria has been approved and recently recommended for wide administration by the WHO, most other zoonotic parasitic diseases lack of appropriate immunoprophylaxis. Sequencing technologies, bioinformatics, and statistics, have opened the “omics” era into apicomplexan parasites, which has led to the development of systems biology, a recent field that can significantly contribute to more rational design for new vaccines. The discovery of novel antigens by classical approaches is slow and limited to very few antigens identified and analyzed by each study. High throughput approaches based on the expansion of the “omics”, mainly genomics and transcriptomics have facilitated the functional annotation of the genome for many of these parasites, improving significantly the understanding of the parasite biology, interactions with the host, as well as virulence and host immune response. Developments in genetic manipulation in apicomplexan parasites have also contributed to the discovery of new potential vaccine targets. The present minireview does a comprehensive summary of advances in “omics”, CRISPR/Cas9 technologies, and in systems biology approaches applied to apicomplexan parasites of economic and zoonotic importance, highlighting their potential of the holistic view in vaccine development.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA127292023-07-05T17:11:54Z Next-generation technologies and systems biology for the design of novel vaccines against apicomplexan parasites Tomazic, Mariela Luján Marugán-Hernandez, Virginia Rodriguez, Anabel Elisa Sporozoa CRISPR Vaccines Parasites Biotechnology Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Vacuna Parásitos Biotecnología Omics Omica Parasites of the phylum Apicomplexa are the causative agents of important diseases such as malaria, toxoplasmosis or cryptosporidiosis in humans, and babesiosis and coccidiosis in animals. Whereas the first human recombinant vaccine against malaria has been approved and recently recommended for wide administration by the WHO, most other zoonotic parasitic diseases lack of appropriate immunoprophylaxis. Sequencing technologies, bioinformatics, and statistics, have opened the “omics” era into apicomplexan parasites, which has led to the development of systems biology, a recent field that can significantly contribute to more rational design for new vaccines. The discovery of novel antigens by classical approaches is slow and limited to very few antigens identified and analyzed by each study. High throughput approaches based on the expansion of the “omics”, mainly genomics and transcriptomics have facilitated the functional annotation of the genome for many of these parasites, improving significantly the understanding of the parasite biology, interactions with the host, as well as virulence and host immune response. Developments in genetic manipulation in apicomplexan parasites have also contributed to the discovery of new potential vaccine targets. The present minireview does a comprehensive summary of advances in “omics”, CRISPR/Cas9 technologies, and in systems biology approaches applied to apicomplexan parasites of economic and zoonotic importance, highlighting their potential of the holistic view in vaccine development. Instituto de Patobiología Fil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Tomazic, Mariela Luján. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tomazic, Mariela Luján. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Catedra de Biotecnología; Argentina Fil: Marugan-Hernandez, Virginia. University of London. The Royal Veterinary College; Reino Unido Fil: Rodriguez, Anabel Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Rodriguez, Anabel Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2022-08-30T16:32:59Z 2022-08-30T16:32:59Z 2022-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12729 https://www.frontiersin.org/articles/10.3389/fvets.2021.800361/full 2297-1769 https://doi.org/10.3389/fvets.2021.800361 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I103-001/2019-PD-E5-I103-001/AR./Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud pública info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001/AR./Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco “Una Salud” info:eu-repograntAgreement/INTA/2019-PD-E5-I102-001/2019-PD-E5-I102-001/AR./Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Veterinary Science 8 : 800361 (Enero 2022)
spellingShingle Sporozoa
CRISPR
Vaccines
Parasites
Biotechnology
Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas
Vacuna
Parásitos
Biotecnología
Omics
Omica
Tomazic, Mariela Luján
Marugán-Hernandez, Virginia
Rodriguez, Anabel Elisa
Next-generation technologies and systems biology for the design of novel vaccines against apicomplexan parasites
title Next-generation technologies and systems biology for the design of novel vaccines against apicomplexan parasites
title_full Next-generation technologies and systems biology for the design of novel vaccines against apicomplexan parasites
title_fullStr Next-generation technologies and systems biology for the design of novel vaccines against apicomplexan parasites
title_full_unstemmed Next-generation technologies and systems biology for the design of novel vaccines against apicomplexan parasites
title_short Next-generation technologies and systems biology for the design of novel vaccines against apicomplexan parasites
title_sort next generation technologies and systems biology for the design of novel vaccines against apicomplexan parasites
topic Sporozoa
CRISPR
Vaccines
Parasites
Biotechnology
Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas
Vacuna
Parásitos
Biotecnología
Omics
Omica
url http://hdl.handle.net/20.500.12123/12729
https://www.frontiersin.org/articles/10.3389/fvets.2021.800361/full
https://doi.org/10.3389/fvets.2021.800361
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