Ectomycorrhizal fungal communities in Nothofagus nervosa [Rauli] : a comparison between domesticated and naturally established specimens in a native forest of Patagonia, Argentina

Due to its overexploitation during the past century, Nothofagus nervosa is currently included in conservation and domestication programs, in which ectomycorrhizas play an important role. We aimed to describe the abundance and diversity of ectomycorrhizal fungi (EcMF) in both domesticated and natural...

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Main Authors: Fernandez, Natalia Verónica, Marchelli, Paula, Gherghel, Felicia, Kost, Gerhard, Fontenla, Sonia Beatriz
Format: Artículo
Language:Inglés
Published: 2017
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/1272
http://www.sciencedirect.com/science/article/pii/S1754504815000653
https://doi.org/10.1016/j.funeco.2015.05.011
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author Fernandez, Natalia Verónica
Marchelli, Paula
Gherghel, Felicia
Kost, Gerhard
Fontenla, Sonia Beatriz
author_browse Fernandez, Natalia Verónica
Fontenla, Sonia Beatriz
Gherghel, Felicia
Kost, Gerhard
Marchelli, Paula
author_facet Fernandez, Natalia Verónica
Marchelli, Paula
Gherghel, Felicia
Kost, Gerhard
Fontenla, Sonia Beatriz
author_sort Fernandez, Natalia Verónica
collection INTA Digital
description Due to its overexploitation during the past century, Nothofagus nervosa is currently included in conservation and domestication programs, in which ectomycorrhizas play an important role. We aimed to describe the abundance and diversity of ectomycorrhizal fungi (EcMF) in both domesticated and naturally established N. nervosa specimens, and to analyse the influence of age, seasonality and forest management on EcMF communities. The occurrence of arbuscular mycorrhizas (AM) and dark septate endophytes (DSE) was also investigated. Fungal diversity and taxonomic identification were assessed by morphotyping and subsequent ITS-rDNA sequencing. Plant age, seasonality and forest management influenced EcMF communities. Colonization rates were higher than 90 % in all the specimens, and were significantly higher in mature trees and in autumn. The highest EcMF richness and diversity values were registered in domesticated specimens and in autumn. Most EcMF were basidiomycetes, belonging mainly to the Cortinariaceae and Tricholomataceae. Arbuscular mycorrhizas were not detected, while DSE were present within N. nervosa roots. Our results and previously published reports showed that some EcMF are capable of colonizing different Nothofagus species. In addition, the EcMF described in natural ecosystems are different from those colonizing N. nervosa during its cultivation in the nursery. These results improve our understanding of key factors affecting EcMF communities associated with Nothofagus in native forests and nurseries (age, season, forest management, cultivation techniques), and this information is relevant for improving domestication programs.
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spelling INTA12722018-06-29T14:37:50Z Ectomycorrhizal fungal communities in Nothofagus nervosa [Rauli] : a comparison between domesticated and naturally established specimens in a native forest of Patagonia, Argentina Fernandez, Natalia Verónica Marchelli, Paula Gherghel, Felicia Kost, Gerhard Fontenla, Sonia Beatriz Nothofagus Hongos Bosque Primario Ectomicorrizas Fungi Ectomycorrhizae Región Patagónica Rauli Nothofagus Nervosa Bosque Nativo Due to its overexploitation during the past century, Nothofagus nervosa is currently included in conservation and domestication programs, in which ectomycorrhizas play an important role. We aimed to describe the abundance and diversity of ectomycorrhizal fungi (EcMF) in both domesticated and naturally established N. nervosa specimens, and to analyse the influence of age, seasonality and forest management on EcMF communities. The occurrence of arbuscular mycorrhizas (AM) and dark septate endophytes (DSE) was also investigated. Fungal diversity and taxonomic identification were assessed by morphotyping and subsequent ITS-rDNA sequencing. Plant age, seasonality and forest management influenced EcMF communities. Colonization rates were higher than 90 % in all the specimens, and were significantly higher in mature trees and in autumn. The highest EcMF richness and diversity values were registered in domesticated specimens and in autumn. Most EcMF were basidiomycetes, belonging mainly to the Cortinariaceae and Tricholomataceae. Arbuscular mycorrhizas were not detected, while DSE were present within N. nervosa roots. Our results and previously published reports showed that some EcMF are capable of colonizing different Nothofagus species. In addition, the EcMF described in natural ecosystems are different from those colonizing N. nervosa during its cultivation in the nursery. These results improve our understanding of key factors affecting EcMF communities associated with Nothofagus in native forests and nurseries (age, season, forest management, cultivation techniques), and this information is relevant for improving domestication programs. Fil: Fernández, Natalia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina Fil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Unidad de Genética Ecológica y Mejoramiento Forestal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gherghel, Felicia. Philipps-Universitat Marburg. Department of Mycology; Alemania Fil: Kost, Gerhard. Philipps-Universitat Marburg. Department of Mycology; Alemania Fil: Fontenla, Sonia Beatriz. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Laboratorio de Microbiología Aplicada y Biotecnología; Argentina 2017-09-20T15:10:23Z 2017-09-20T15:10:23Z 2015-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1272 http://www.sciencedirect.com/science/article/pii/S1754504815000653 1754-5048 https://doi.org/10.1016/j.funeco.2015.05.011 eng info:eu-repo/semantics/restrictedAccess application/pdf Patagonia (general region) Fungal ecology 18 : 36-47.(December 2015)
spellingShingle Nothofagus
Hongos
Bosque Primario
Ectomicorrizas
Fungi
Ectomycorrhizae
Región Patagónica
Rauli
Nothofagus Nervosa
Bosque Nativo
Fernandez, Natalia Verónica
Marchelli, Paula
Gherghel, Felicia
Kost, Gerhard
Fontenla, Sonia Beatriz
Ectomycorrhizal fungal communities in Nothofagus nervosa [Rauli] : a comparison between domesticated and naturally established specimens in a native forest of Patagonia, Argentina
title Ectomycorrhizal fungal communities in Nothofagus nervosa [Rauli] : a comparison between domesticated and naturally established specimens in a native forest of Patagonia, Argentina
title_full Ectomycorrhizal fungal communities in Nothofagus nervosa [Rauli] : a comparison between domesticated and naturally established specimens in a native forest of Patagonia, Argentina
title_fullStr Ectomycorrhizal fungal communities in Nothofagus nervosa [Rauli] : a comparison between domesticated and naturally established specimens in a native forest of Patagonia, Argentina
title_full_unstemmed Ectomycorrhizal fungal communities in Nothofagus nervosa [Rauli] : a comparison between domesticated and naturally established specimens in a native forest of Patagonia, Argentina
title_short Ectomycorrhizal fungal communities in Nothofagus nervosa [Rauli] : a comparison between domesticated and naturally established specimens in a native forest of Patagonia, Argentina
title_sort ectomycorrhizal fungal communities in nothofagus nervosa rauli a comparison between domesticated and naturally established specimens in a native forest of patagonia argentina
topic Nothofagus
Hongos
Bosque Primario
Ectomicorrizas
Fungi
Ectomycorrhizae
Región Patagónica
Rauli
Nothofagus Nervosa
Bosque Nativo
url http://hdl.handle.net/20.500.12123/1272
http://www.sciencedirect.com/science/article/pii/S1754504815000653
https://doi.org/10.1016/j.funeco.2015.05.011
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