Population replacement of benzimidazole-resistant Haemonchus contortus with susceptible strains: evidence of changes in the resistance status
The spread of anthelmintic resistance (AR) in nematode populations threatens the viability of sheep production systems worldwide, and warrants the adoption of sensitive, practical, and standardized tests to detect AR. The aim of this study was to characterize the replacement of an Haemonchus contort...
| Autores principales: | , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/12628 https://link.springer.com/article/10.1007/s00436-022-07582-9 https://doi.org/10.1007/s00436-022-07582-9 |
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| author | Muchiut, Sebastián Fiel, César Alberto Lirón, Juan Pedro Lloberas, María Mercedes Ceriani, Carolina Lorenzo, Ramiro Riva, Eliana Bernat, Gisele Anahí Cardozo, Patricia Alejandra Fernández, Silvina Steffan, Pedro |
| author_browse | Bernat, Gisele Anahí Cardozo, Patricia Alejandra Ceriani, Carolina Fernández, Silvina Fiel, César Alberto Lirón, Juan Pedro Lloberas, María Mercedes Lorenzo, Ramiro Muchiut, Sebastián Riva, Eliana Steffan, Pedro |
| author_facet | Muchiut, Sebastián Fiel, César Alberto Lirón, Juan Pedro Lloberas, María Mercedes Ceriani, Carolina Lorenzo, Ramiro Riva, Eliana Bernat, Gisele Anahí Cardozo, Patricia Alejandra Fernández, Silvina Steffan, Pedro |
| author_sort | Muchiut, Sebastián |
| collection | INTA Digital |
| description | The spread of anthelmintic resistance (AR) in nematode populations threatens the viability of sheep production systems worldwide, and warrants the adoption of sensitive, practical, and standardized tests to detect AR. The aim of this study was to characterize the replacement of an Haemonchus contortus population resistant to benzimidazoles (BZDs) by a susceptible one, by means of both phenotypic and genotypic techniques. Phenotypic methods to assess BZD resistance included in vivo tests, such as the fecal egg count reduction test (FECRT), and in vitro tests, such as the egg hatch assay (EHA). Additionally, genotypification of polymorphisms associated with BZD resistance by sequencing a fragment of the isotype 1 β-tubulin gene was carried out. The initial, BZD-resistant population (initial Balcarce population) exhibited an egg count reduction (ECR) of 59.3%. Following refugium replacement, the final population (final Balcarce population) exhibited an ECR of 95.2%. For the initial Balcarce population, the median effective dose (ED50) for the EHA was 0.607 μg thiabendazole (TBZ)/mL, with a rate of eclosion at a discriminating dose (EDD) of 0.1 μg TBZ/mL of 76.73%. For the final Balcarce population, ED50 was 0.02 μg TBZ/mL, and EDD was 1.97%. In the initial population, 93% of the analyzed individuals exhibited genotypic combinations associated with BZD resistance (53% Phe/Phe167–Tyr/Tyr200, 37% Phe/Tyr167–Phe/Tyr200, and 3% Phe/Tyr167–Glu/Leu198). Conversely, no combination associated with resistance was found in individuals from the final population. All of the tests were useful for detecting AR to BZDs. The results from the genetic and phenotypical studies were consistent, and the resulting information greatly aided in interpreting the outcomes of the population replacement and the potential impact of this strategy on management of AR. |
| format | info:ar-repo/semantics/artículo |
| id | INTA12628 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA126282025-05-13T11:10:48Z Population replacement of benzimidazole-resistant Haemonchus contortus with susceptible strains: evidence of changes in the resistance status Muchiut, Sebastián Fiel, César Alberto Lirón, Juan Pedro Lloberas, María Mercedes Ceriani, Carolina Lorenzo, Ramiro Riva, Eliana Bernat, Gisele Anahí Cardozo, Patricia Alejandra Fernández, Silvina Steffan, Pedro Haemonchus Contortus Resistencia a los Antihelmíncos Diagnóstico Nematoda Resistance to Anthelmintics Benzimidazoles Diagnosis The spread of anthelmintic resistance (AR) in nematode populations threatens the viability of sheep production systems worldwide, and warrants the adoption of sensitive, practical, and standardized tests to detect AR. The aim of this study was to characterize the replacement of an Haemonchus contortus population resistant to benzimidazoles (BZDs) by a susceptible one, by means of both phenotypic and genotypic techniques. Phenotypic methods to assess BZD resistance included in vivo tests, such as the fecal egg count reduction test (FECRT), and in vitro tests, such as the egg hatch assay (EHA). Additionally, genotypification of polymorphisms associated with BZD resistance by sequencing a fragment of the isotype 1 β-tubulin gene was carried out. The initial, BZD-resistant population (initial Balcarce population) exhibited an egg count reduction (ECR) of 59.3%. Following refugium replacement, the final population (final Balcarce population) exhibited an ECR of 95.2%. For the initial Balcarce population, the median effective dose (ED50) for the EHA was 0.607 μg thiabendazole (TBZ)/mL, with a rate of eclosion at a discriminating dose (EDD) of 0.1 μg TBZ/mL of 76.73%. For the final Balcarce population, ED50 was 0.02 μg TBZ/mL, and EDD was 1.97%. In the initial population, 93% of the analyzed individuals exhibited genotypic combinations associated with BZD resistance (53% Phe/Phe167–Tyr/Tyr200, 37% Phe/Tyr167–Phe/Tyr200, and 3% Phe/Tyr167–Glu/Leu198). Conversely, no combination associated with resistance was found in individuals from the final population. All of the tests were useful for detecting AR to BZDs. The results from the genetic and phenotypical studies were consistent, and the resulting information greatly aided in interpreting the outcomes of the population replacement and the potential impact of this strategy on management of AR. EEA Balcarce Fil: Muchiut, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Fiel, César. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Lirón, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Lloberas, Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Ceriani, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Lorenzo, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Riva, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Bernat, Gisele. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Cardozo, Patricia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Fernández, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Steffan, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. 2022-08-19T10:28:16Z 2022-08-19T10:28:16Z 2022-07-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12628 https://link.springer.com/article/10.1007/s00436-022-07582-9 1432-1955 (online) 0932-0113 (print) https://doi.org/10.1007/s00436-022-07582-9 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Parasitology Research 121 : 2623–2632 (July 2022) |
| spellingShingle | Haemonchus Contortus Resistencia a los Antihelmíncos Diagnóstico Nematoda Resistance to Anthelmintics Benzimidazoles Diagnosis Muchiut, Sebastián Fiel, César Alberto Lirón, Juan Pedro Lloberas, María Mercedes Ceriani, Carolina Lorenzo, Ramiro Riva, Eliana Bernat, Gisele Anahí Cardozo, Patricia Alejandra Fernández, Silvina Steffan, Pedro Population replacement of benzimidazole-resistant Haemonchus contortus with susceptible strains: evidence of changes in the resistance status |
| title | Population replacement of benzimidazole-resistant Haemonchus contortus with susceptible strains: evidence of changes in the resistance status |
| title_full | Population replacement of benzimidazole-resistant Haemonchus contortus with susceptible strains: evidence of changes in the resistance status |
| title_fullStr | Population replacement of benzimidazole-resistant Haemonchus contortus with susceptible strains: evidence of changes in the resistance status |
| title_full_unstemmed | Population replacement of benzimidazole-resistant Haemonchus contortus with susceptible strains: evidence of changes in the resistance status |
| title_short | Population replacement of benzimidazole-resistant Haemonchus contortus with susceptible strains: evidence of changes in the resistance status |
| title_sort | population replacement of benzimidazole resistant haemonchus contortus with susceptible strains evidence of changes in the resistance status |
| topic | Haemonchus Contortus Resistencia a los Antihelmíncos Diagnóstico Nematoda Resistance to Anthelmintics Benzimidazoles Diagnosis |
| url | http://hdl.handle.net/20.500.12123/12628 https://link.springer.com/article/10.1007/s00436-022-07582-9 https://doi.org/10.1007/s00436-022-07582-9 |
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