Relationship of Salmonella infection and inflammatory intestinal response with hematological and serum biochemical values in laying hens
There are few studies about the blood serum of laying hens infected with Salmonella. The differential leukocyte count and blood chemistry values are an important aid in the diagnosis of human diseases, but blood parameters in the avian species are not well known. On the other hand, invasive forms of...
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1254 http://www.sciencedirect.com/science/article/pii/S016524271500077X https://doi.org/10.1016/j.vetimm.2015.03.008 |
| _version_ | 1855482856152760320 |
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| author | Soria, Mario Bonnet, María Agustina Bueno, Dante Javier |
| author_browse | Bonnet, María Agustina Bueno, Dante Javier Soria, Mario |
| author_facet | Soria, Mario Bonnet, María Agustina Bueno, Dante Javier |
| author_sort | Soria, Mario |
| collection | INTA Digital |
| description | There are few studies about the blood serum of laying hens infected with Salmonella. The differential leukocyte count and blood chemistry values are an important aid in the diagnosis of human diseases, but blood parameters in the avian species are not well known. On the other hand, invasive forms of bacterial gastroenteritis, like Salmonella, often cause intestinal inflammation so this study was undertaken to find a biomarker of Salmonella infection and inflammatory intestinal response in the hematological or serum biochemical parameters in laying hens. Furthermore, we evaluated the association of some farm characteristics with Salmonella infection and fecal leukocytes (FL). A fecal sample with at least one fecal leukocyte per field was considered positive for inflammatory intestinal response. False positive serum reactions for Salmonella infection, by serum plate agglutination (SPA) test, were reduced by heating the sample to 56 °C for 30 min and then diluting it 5-fold. The range of hematological and biochemical parameter values was very wide, in addition, there was a poor agreement between the SPA and FL results. Comparison of the positive and negative samples in SPA and FL showed that 1.3% and 79.8% of the laying hens were positive and negative in both tests, respectively. Hens with a positive SPA result showed a higher percentage of monocytes than those with a negative SPA result. Hens with a positive FL test had a higher percentage of heterophils, ratio of heterophils to lymphocytes and aspartate aminotransferase values, while the percentage of lymphocytes was significantly lower (P < 0.05) than those with a negative FL test. The risk of Salmonella infection increased when the age of laying hens and the number of hens per poultry house was greater than or equal to 18 months old and 10,000 laying hens, compared to less than 18 months old and 10,000 laying hens, respectively. On the other hand, the risk of inflammatory intestinal response was higher in laying hens ≥18 months old than in hens <18 months old. Despite the fact that we did not find any specific biomarker of Salmonella infection, this is the first report about the change of Salmonella infection and inflammatory response in hematological/serum biochemical values for laying hens. |
| format | Artículo |
| id | INTA1254 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA12542022-03-31T13:38:55Z Relationship of Salmonella infection and inflammatory intestinal response with hematological and serum biochemical values in laying hens Soria, Mario Bonnet, María Agustina Bueno, Dante Javier Gallina Ponedora Layer Chickens Infection Leukocytes Intestines Biological Analysis Salmonella Infección Leucocitos Intestinos Análisis Biológico There are few studies about the blood serum of laying hens infected with Salmonella. The differential leukocyte count and blood chemistry values are an important aid in the diagnosis of human diseases, but blood parameters in the avian species are not well known. On the other hand, invasive forms of bacterial gastroenteritis, like Salmonella, often cause intestinal inflammation so this study was undertaken to find a biomarker of Salmonella infection and inflammatory intestinal response in the hematological or serum biochemical parameters in laying hens. Furthermore, we evaluated the association of some farm characteristics with Salmonella infection and fecal leukocytes (FL). A fecal sample with at least one fecal leukocyte per field was considered positive for inflammatory intestinal response. False positive serum reactions for Salmonella infection, by serum plate agglutination (SPA) test, were reduced by heating the sample to 56 °C for 30 min and then diluting it 5-fold. The range of hematological and biochemical parameter values was very wide, in addition, there was a poor agreement between the SPA and FL results. Comparison of the positive and negative samples in SPA and FL showed that 1.3% and 79.8% of the laying hens were positive and negative in both tests, respectively. Hens with a positive SPA result showed a higher percentage of monocytes than those with a negative SPA result. Hens with a positive FL test had a higher percentage of heterophils, ratio of heterophils to lymphocytes and aspartate aminotransferase values, while the percentage of lymphocytes was significantly lower (P < 0.05) than those with a negative FL test. The risk of Salmonella infection increased when the age of laying hens and the number of hens per poultry house was greater than or equal to 18 months old and 10,000 laying hens, compared to less than 18 months old and 10,000 laying hens, respectively. On the other hand, the risk of inflammatory intestinal response was higher in laying hens ≥18 months old than in hens <18 months old. Despite the fact that we did not find any specific biomarker of Salmonella infection, this is the first report about the change of Salmonella infection and inflammatory response in hematological/serum biochemical values for laying hens. Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Bonnet, María Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina 2017-09-19T15:18:04Z 2017-09-19T15:18:04Z 2015-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1254 http://www.sciencedirect.com/science/article/pii/S016524271500077X 0165-2427 https://doi.org/10.1016/j.vetimm.2015.03.008 eng info:eu-repo/semantics/restrictedAccess application/pdf Veterinary immunology and immunopatology 165 (3-4) : 145-153. (June 2015) |
| spellingShingle | Gallina Ponedora Layer Chickens Infection Leukocytes Intestines Biological Analysis Salmonella Infección Leucocitos Intestinos Análisis Biológico Soria, Mario Bonnet, María Agustina Bueno, Dante Javier Relationship of Salmonella infection and inflammatory intestinal response with hematological and serum biochemical values in laying hens |
| title | Relationship of Salmonella infection and inflammatory intestinal response with hematological and serum biochemical values in laying hens |
| title_full | Relationship of Salmonella infection and inflammatory intestinal response with hematological and serum biochemical values in laying hens |
| title_fullStr | Relationship of Salmonella infection and inflammatory intestinal response with hematological and serum biochemical values in laying hens |
| title_full_unstemmed | Relationship of Salmonella infection and inflammatory intestinal response with hematological and serum biochemical values in laying hens |
| title_short | Relationship of Salmonella infection and inflammatory intestinal response with hematological and serum biochemical values in laying hens |
| title_sort | relationship of salmonella infection and inflammatory intestinal response with hematological and serum biochemical values in laying hens |
| topic | Gallina Ponedora Layer Chickens Infection Leukocytes Intestines Biological Analysis Salmonella Infección Leucocitos Intestinos Análisis Biológico |
| url | http://hdl.handle.net/20.500.12123/1254 http://www.sciencedirect.com/science/article/pii/S016524271500077X https://doi.org/10.1016/j.vetimm.2015.03.008 |
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