Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil
Genotyping methods have led to a better understanding of the epidemiology of Mycobacterium bovis (M. bovis) infection, and its transmission dynamics, as well as the possible phylogenetic relationships between Mycobacterium strains, thus making bovine tuberculosis control programs more efficient. The...
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/12493 https://link.springer.com/article/10.1007/s11250-022-03243-2 https://doi.org/10.1007/s11250-022-03243-2 |
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| author | Souza, Mariana Assunção de Lopes, Thaís Aline Carolino Silva, Brendhal Almeida Bombonato, Nadia Grandi Dib, Cristina Corsi Marfil, Maria Jimena Zumarraga, Martin Jose Lima, Anna Monteiro Correia |
| author_browse | Bombonato, Nadia Grandi Dib, Cristina Corsi Lima, Anna Monteiro Correia Lopes, Thaís Aline Carolino Marfil, Maria Jimena Silva, Brendhal Almeida Souza, Mariana Assunção de Zumarraga, Martin Jose |
| author_facet | Souza, Mariana Assunção de Lopes, Thaís Aline Carolino Silva, Brendhal Almeida Bombonato, Nadia Grandi Dib, Cristina Corsi Marfil, Maria Jimena Zumarraga, Martin Jose Lima, Anna Monteiro Correia |
| author_sort | Souza, Mariana Assunção de |
| collection | INTA Digital |
| description | Genotyping methods have led to a better understanding of the epidemiology of Mycobacterium bovis (M. bovis) infection, and its transmission dynamics, as well as the possible phylogenetic relationships between Mycobacterium strains, thus making bovine tuberculosis control programs more efficient. The goal of this study was to characterize the main spoligotypes of M. bovis isolated from cattle in the State of Minas Gerais, Brazil. It was carried out in 28 municipalities of “Triângulo Mineiro” and “Alto Paranaíba” regions of the state. Viscera samples were obtained from 58 bovines positive for tuberculosis according to comparative cervical tests, and from another 100 bovines with lesions suggestive of tuberculosis, which were donated by the National Agricultural Laboratory of Pedro Leopoldo, Minas Gerais. Microbiological isolation was performed in Stonebrink medium, and molecular identification of mycobacteria was performed by PCR. Genotyping was performed using the spoligotyping method at the Agrobiotechnology and Molecular Biology Institute of National Agricultural Technology Institute-National Scientific and Technical Research Council, Buenos Aires, Argentina. Among the 158 viscera samples, we obtained 40 (25%) isolates of M. bovis, and detected 11 spoligotype patterns, with a predominance of SB1142 (37.5%), SB0121 (25.0%), and SB1145 (10.0%). Other standards, SB0295, SB1050, SB0881, SB1144, SB1802, SB0140, SB0120, and SB0849, varied from 2.5 to 7.5%, heterogeneously distributed among the municipalities. The presence of spoligotypes shared with other Brazilian states and different countries indicates their possible exchange through epidemiological relationships, such as the transit of live animals and/or genetic similarity between strains that share a common ancestor. |
| format | Artículo |
| id | INTA12493 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA124932022-08-04T11:36:44Z Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil Souza, Mariana Assunção de Lopes, Thaís Aline Carolino Silva, Brendhal Almeida Bombonato, Nadia Grandi Dib, Cristina Corsi Marfil, Maria Jimena Zumarraga, Martin Jose Lima, Anna Monteiro Correia Epidemiology Tuberculosis Cattle Genotyping Brazil Epidemiología Bovinae Mycobacterium bovis Ganado Bovino Genotipado Brasil Spoligotypes Espoligotipos Genotyping methods have led to a better understanding of the epidemiology of Mycobacterium bovis (M. bovis) infection, and its transmission dynamics, as well as the possible phylogenetic relationships between Mycobacterium strains, thus making bovine tuberculosis control programs more efficient. The goal of this study was to characterize the main spoligotypes of M. bovis isolated from cattle in the State of Minas Gerais, Brazil. It was carried out in 28 municipalities of “Triângulo Mineiro” and “Alto Paranaíba” regions of the state. Viscera samples were obtained from 58 bovines positive for tuberculosis according to comparative cervical tests, and from another 100 bovines with lesions suggestive of tuberculosis, which were donated by the National Agricultural Laboratory of Pedro Leopoldo, Minas Gerais. Microbiological isolation was performed in Stonebrink medium, and molecular identification of mycobacteria was performed by PCR. Genotyping was performed using the spoligotyping method at the Agrobiotechnology and Molecular Biology Institute of National Agricultural Technology Institute-National Scientific and Technical Research Council, Buenos Aires, Argentina. Among the 158 viscera samples, we obtained 40 (25%) isolates of M. bovis, and detected 11 spoligotype patterns, with a predominance of SB1142 (37.5%), SB0121 (25.0%), and SB1145 (10.0%). Other standards, SB0295, SB1050, SB0881, SB1144, SB1802, SB0140, SB0120, and SB0849, varied from 2.5 to 7.5%, heterogeneously distributed among the municipalities. The presence of spoligotypes shared with other Brazilian states and different countries indicates their possible exchange through epidemiological relationships, such as the transit of live animals and/or genetic similarity between strains that share a common ancestor. Instituto de Biotecnología Fil: de Souza, Mariana Assunção. Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Laboratório de Doenças Infectocontagiosas; Brasil Fil: Lopes, Thaís Aline Carolino. Centro Universitário de Patos de Minas. Faculdade de Medicina Veterinária; Brasil Fil: Silva, Brendhal Almeida. Centro Universitário de Patos de Minas. Faculdade de Medicina Veterinária; Brasil Fil: Bombonato, Nadia Grandi. Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Laboratório de Doenças Infectocontagiosas; Brasil Fil: Dib, Cristina Corsi. Instituto Biológico de São Paulo. Laboratório de Tuberculose; Brasil Fil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Marfil, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lima, Anna Monteiro Correia. Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Laboratório de Doenças Infectocontagiosas; Brasil 2022-08-04T11:29:23Z 2022-08-04T11:29:23Z 2022-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12493 https://link.springer.com/article/10.1007/s11250-022-03243-2 1573-7438 https://doi.org/10.1007/s11250-022-03243-2 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I103-001/2019-PD-E5-I103-001/AR./Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud pública info:eu-repo/semantics/restrictedAccess application/pdf Springer Tropical Animal Health and Production 54 (4) : 238 (Julio 2022) |
| spellingShingle | Epidemiology Tuberculosis Cattle Genotyping Brazil Epidemiología Bovinae Mycobacterium bovis Ganado Bovino Genotipado Brasil Spoligotypes Espoligotipos Souza, Mariana Assunção de Lopes, Thaís Aline Carolino Silva, Brendhal Almeida Bombonato, Nadia Grandi Dib, Cristina Corsi Marfil, Maria Jimena Zumarraga, Martin Jose Lima, Anna Monteiro Correia Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil |
| title | Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil |
| title_full | Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil |
| title_fullStr | Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil |
| title_full_unstemmed | Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil |
| title_short | Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil |
| title_sort | spatial distribution of mycobacterium bovis spoligotypes in cattle from the state of minas gerais brazil |
| topic | Epidemiology Tuberculosis Cattle Genotyping Brazil Epidemiología Bovinae Mycobacterium bovis Ganado Bovino Genotipado Brasil Spoligotypes Espoligotipos |
| url | http://hdl.handle.net/20.500.12123/12493 https://link.springer.com/article/10.1007/s11250-022-03243-2 https://doi.org/10.1007/s11250-022-03243-2 |
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