Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance
Drought is one of the main environmental stresses that negatively impacts vegetative and reproductive yield. Water deficit responses are determined by the duration and intensity of the stress, which, together with plant genotype, will define the chances of plant survival. The metabolic adjustments i...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/12491 https://link.springer.com/article/10.1007/s11103-022-01273-w https://doi.org/10.1007/s11103-022-01273-w |
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| author | Suguiyama, Vanessa Fuentes Rodriguez, Jae Diana Paredes Santos, Tatiane Cristina Nicomedio dos Lira, Bruno Silvestre de Haro, Luis Alejandro Silva, João Paulo Naldi Borba, Eduardo Leite Purgatto, Eduardo Silva, Emerson Alves da Bellora Pereyra, Nicolás Carrari, Fernando Centeno, Danilo da Cruz Bermudez Salazar, Luisa Rossi, Magdalena de Setta, Nathalia |
| author_browse | Bellora Pereyra, Nicolás Bermudez Salazar, Luisa Borba, Eduardo Leite Carrari, Fernando Centeno, Danilo da Cruz Lira, Bruno Silvestre Purgatto, Eduardo Rodriguez, Jae Diana Paredes Rossi, Magdalena Santos, Tatiane Cristina Nicomedio dos Silva, Emerson Alves da Silva, João Paulo Naldi Suguiyama, Vanessa Fuentes de Haro, Luis Alejandro de Setta, Nathalia |
| author_facet | Suguiyama, Vanessa Fuentes Rodriguez, Jae Diana Paredes Santos, Tatiane Cristina Nicomedio dos Lira, Bruno Silvestre de Haro, Luis Alejandro Silva, João Paulo Naldi Borba, Eduardo Leite Purgatto, Eduardo Silva, Emerson Alves da Bellora Pereyra, Nicolás Carrari, Fernando Centeno, Danilo da Cruz Bermudez Salazar, Luisa Rossi, Magdalena de Setta, Nathalia |
| author_sort | Suguiyama, Vanessa Fuentes |
| collection | INTA Digital |
| description | Drought is one of the main environmental stresses that negatively impacts vegetative and reproductive yield. Water deficit responses are determined by the duration and intensity of the stress, which, together with plant genotype, will define the chances of plant survival. The metabolic adjustments in response to water deficit are complex and involve gene expression modulation regulated by DNA-binding proteins and epigenetic modifications. This last mechanism may also regulate the activity of transposable elements, which in turn impact the expression of nearby loci. Setaria italica plants submitted to five water deficit regimes were analyzed through a phenotypical approach, including growth, physiological, RNA-seq and sRNA-seq analyses. The results showed a progressive reduction in yield as a function of water deficit intensity associated with signaling pathway modulation and metabolic adjustments. We identified a group of loci that were consistently associated with drought responses, some of which were related to water deficit perception, signaling and regulation. Finally, an analysis of the transcriptome and sRNAome allowed us to identify genes putatively regulated by TE- and sRNA-related mechanisms and an intriguing positive correlation between transcript levels and sRNA accumulation in gene body regions. These findings shed light on the processes that allow S. italica to overcome drought and survive under water restrictive conditions. |
| format | info:ar-repo/semantics/artículo |
| id | INTA12491 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA124912022-08-11T16:43:11Z Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance Suguiyama, Vanessa Fuentes Rodriguez, Jae Diana Paredes Santos, Tatiane Cristina Nicomedio dos Lira, Bruno Silvestre de Haro, Luis Alejandro Silva, João Paulo Naldi Borba, Eduardo Leite Purgatto, Eduardo Silva, Emerson Alves da Bellora Pereyra, Nicolás Carrari, Fernando Centeno, Danilo da Cruz Bermudez Salazar, Luisa Rossi, Magdalena de Setta, Nathalia Monocotyledons Transcriptome Plant Metabolism Transposons Phenotypic Plasticity Tolerance Monocotiledóneas Transcriptomas Metabolismo de las Plantas Transposones Plasticidad Fenotípica Setaria italica Tolerancia Water Deficit Déficit de Agua Drought is one of the main environmental stresses that negatively impacts vegetative and reproductive yield. Water deficit responses are determined by the duration and intensity of the stress, which, together with plant genotype, will define the chances of plant survival. The metabolic adjustments in response to water deficit are complex and involve gene expression modulation regulated by DNA-binding proteins and epigenetic modifications. This last mechanism may also regulate the activity of transposable elements, which in turn impact the expression of nearby loci. Setaria italica plants submitted to five water deficit regimes were analyzed through a phenotypical approach, including growth, physiological, RNA-seq and sRNA-seq analyses. The results showed a progressive reduction in yield as a function of water deficit intensity associated with signaling pathway modulation and metabolic adjustments. We identified a group of loci that were consistently associated with drought responses, some of which were related to water deficit perception, signaling and regulation. Finally, an analysis of the transcriptome and sRNAome allowed us to identify genes putatively regulated by TE- and sRNA-related mechanisms and an intriguing positive correlation between transcript levels and sRNA accumulation in gene body regions. These findings shed light on the processes that allow S. italica to overcome drought and survive under water restrictive conditions. Instituto de Biotecnología Fil: Suguiyama, Vanessa Fuentes. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil Fil: Paredes Rodriguez, Jae Diana. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil Fil: dos Santos, Tatiane Cristina Nicomedio. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil Fil: Lira, Bruno Silvestre. Universidade de São Pablo. Instituto de Biociencias. Departamento de Botânica; Brasil Fil: de Haro, Luis Alejandro. Weizmann Institute of Science. Departament of Plant and Environmental Sciences; Israel Fil: Silva, João Paulo Naldi. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil Fil: Borba, Eduardo Leite. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Botânica; Brasil Fil: Purgatto, Eduardo. Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Alimentos e Nutrição Experimental; Brasil Fil: da Silva, Emerson Alves. Secretaria do Meio Ambiente do Estado de São Paulo. Instituto de Botânica; Brasil Fil: Bellora, Nicolas. Instituto de Tecnologías Nucleares para la Salud (INTECNUS); Argentina Fil: Bellora, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología Y Biología Molecular; Argentina Fil: Carrari, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carrari, Fernando. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Genética; Argentina Fil: da Cruz Centeno, Danilo. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil Fil: Bermudez Salazar, Luisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Bermudez Salazar, Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bermudez Salazar, Luisa. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Genética; Argentina Fil: Rossi, Magdalena. Universidade de São Pablo. Instituto de Biociencias. Departamento de Botânica; Brasil Fil: de Setta, Nathalia. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil 2022-08-04T11:05:11Z 2022-08-04T11:05:11Z 2022-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12491 https://link.springer.com/article/10.1007/s11103-022-01273-w 1573-5028 0167-4412 https://doi.org/10.1007/s11103-022-01273-w eng info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001/2019-PD-E6-I116-001/AR./Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial. info:eu-repo/semantics/restrictedAccess application/pdf Springer Plant Molecular Biology 109 : 761-780. (2022) |
| spellingShingle | Monocotyledons Transcriptome Plant Metabolism Transposons Phenotypic Plasticity Tolerance Monocotiledóneas Transcriptomas Metabolismo de las Plantas Transposones Plasticidad Fenotípica Setaria italica Tolerancia Water Deficit Déficit de Agua Suguiyama, Vanessa Fuentes Rodriguez, Jae Diana Paredes Santos, Tatiane Cristina Nicomedio dos Lira, Bruno Silvestre de Haro, Luis Alejandro Silva, João Paulo Naldi Borba, Eduardo Leite Purgatto, Eduardo Silva, Emerson Alves da Bellora Pereyra, Nicolás Carrari, Fernando Centeno, Danilo da Cruz Bermudez Salazar, Luisa Rossi, Magdalena de Setta, Nathalia Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance |
| title | Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance |
| title_full | Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance |
| title_fullStr | Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance |
| title_full_unstemmed | Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance |
| title_short | Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance |
| title_sort | regulatory mechanisms behind the phenotypic plasticity associated with setaria italica water deficit tolerance |
| topic | Monocotyledons Transcriptome Plant Metabolism Transposons Phenotypic Plasticity Tolerance Monocotiledóneas Transcriptomas Metabolismo de las Plantas Transposones Plasticidad Fenotípica Setaria italica Tolerancia Water Deficit Déficit de Agua |
| url | http://hdl.handle.net/20.500.12123/12491 https://link.springer.com/article/10.1007/s11103-022-01273-w https://doi.org/10.1007/s11103-022-01273-w |
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