Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds
Thecaphora frezii is the causal agent of peanut smut, important disease in the Argentine production area. The pathogen can be dispersed by kernels, through surface contamination or small lesions. The aim of this work was to design specific primers to develop a Polymerase Chain Reaction assay for th...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1242 http://sipav.org/main/jpp/index.php/jpp/article/view/3570/2237 http://dx.doi.org/10.4454/JPP.V98I2.034 |
| _version_ | 1855482853807095808 |
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| author | Cazon, Luis Ignacio Conforto, Erica Cinthia Fernandez, Franco Daniel Paredes, Juan Andrés Rago, Alejandro Mario |
| author_browse | Cazon, Luis Ignacio Conforto, Erica Cinthia Fernandez, Franco Daniel Paredes, Juan Andrés Rago, Alejandro Mario |
| author_facet | Cazon, Luis Ignacio Conforto, Erica Cinthia Fernandez, Franco Daniel Paredes, Juan Andrés Rago, Alejandro Mario |
| author_sort | Cazon, Luis Ignacio |
| collection | INTA Digital |
| description | Thecaphora frezii is the causal agent of peanut smut, important disease in the Argentine production area. The pathogen can be dispersed by kernels, through surface contamination or small lesions. The aim of this work was to design specific primers to develop a Polymerase Chain Reaction assay for the detection of Thecaphora frezii in peanuts kernels. The ITS region of four T. frezii isolates was amplified and sequenced. A consensus sequence was obtained and a pair of primers specific for the pathogen, named TF-2F and TF- 2R, was synthesized. The primers were used for specificity and sensitivity tests. The former test involved a PCR using DNA from T. frezii and other seed-transmitted pathogens (S. rolfsi, S. minor, Fusarium solani and S. sclerotiorum). A 190 bp fragment was obtained for the T. frezii isolates, whereas no amplification was observed for the remaining pathogens. For the sensitivity test, positive results were observed at concentrations up to 3x10-4 pg of the target DNA. The specificity of this method, along with its sensitivity, makes it an important tool for epidemiological studies of peanut smut. |
| format | Artículo |
| id | INTA1242 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA12422017-11-17T11:42:09Z Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds Cazon, Luis Ignacio Conforto, Erica Cinthia Fernandez, Franco Daniel Paredes, Juan Andrés Rago, Alejandro Mario Enfermedades de las Plantas Arachis Hypogaea Genética Plant Diseases Genetics Maní Thecaphora Frezzi Thecaphora frezii is the causal agent of peanut smut, important disease in the Argentine production area. The pathogen can be dispersed by kernels, through surface contamination or small lesions. The aim of this work was to design specific primers to develop a Polymerase Chain Reaction assay for the detection of Thecaphora frezii in peanuts kernels. The ITS region of four T. frezii isolates was amplified and sequenced. A consensus sequence was obtained and a pair of primers specific for the pathogen, named TF-2F and TF- 2R, was synthesized. The primers were used for specificity and sensitivity tests. The former test involved a PCR using DNA from T. frezii and other seed-transmitted pathogens (S. rolfsi, S. minor, Fusarium solani and S. sclerotiorum). A 190 bp fragment was obtained for the T. frezii isolates, whereas no amplification was observed for the remaining pathogens. For the sensitivity test, positive results were observed at concentrations up to 3x10-4 pg of the target DNA. The specificity of this method, along with its sensitivity, makes it an important tool for epidemiological studies of peanut smut. Inst. Patología Vegetal Fil: Cazon, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Paredes, Juan Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Rago, Alejandro Mario Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina 2017-09-18T17:05:13Z 2017-09-18T17:05:13Z 2016 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1242 http://sipav.org/main/jpp/index.php/jpp/article/view/3570/2237 1125-4653 http://dx.doi.org/10.4454/JPP.V98I2.034 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Journal of Plant Pathology 98 (2) : 327-330 (2016) |
| spellingShingle | Enfermedades de las Plantas Arachis Hypogaea Genética Plant Diseases Genetics Maní Thecaphora Frezzi Cazon, Luis Ignacio Conforto, Erica Cinthia Fernandez, Franco Daniel Paredes, Juan Andrés Rago, Alejandro Mario Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds |
| title | Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds |
| title_full | Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds |
| title_fullStr | Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds |
| title_full_unstemmed | Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds |
| title_short | Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds |
| title_sort | molecular detection of thecaphora frezzi in peanut arachis hypogaea l seeds |
| topic | Enfermedades de las Plantas Arachis Hypogaea Genética Plant Diseases Genetics Maní Thecaphora Frezzi |
| url | http://hdl.handle.net/20.500.12123/1242 http://sipav.org/main/jpp/index.php/jpp/article/view/3570/2237 http://dx.doi.org/10.4454/JPP.V98I2.034 |
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