Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay
Because some batch-to-batch variation in the preparation of rough lipopolysaccharide (RLPS) from Brucella ovis has been experienced, several protocols were tested to establish the most reliable method for detection of antibody in indirect enzyme immunoassay. An early version of the assay gave a perf...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Taylor & Francis Group
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/12414 https://www.tandfonline.com/doi/abs/10.1080/15321810701454789 https://doi.org/10.1080/15321810701454789 |
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| author | Nielsen, Klaus Smith, P. Yu, W. L. Rojas, X. Perez, B. Conde, Sandra Beatriz Samartino, Luis Ernesto Robles, Carlos Alejandro |
| author_browse | Conde, Sandra Beatriz Nielsen, Klaus Perez, B. Robles, Carlos Alejandro Rojas, X. Samartino, Luis Ernesto Smith, P. Yu, W. L. |
| author_facet | Nielsen, Klaus Smith, P. Yu, W. L. Rojas, X. Perez, B. Conde, Sandra Beatriz Samartino, Luis Ernesto Robles, Carlos Alejandro |
| author_sort | Nielsen, Klaus |
| collection | INTA Digital |
| description | Because some batch-to-batch variation in the preparation of rough lipopolysaccharide (RLPS) from Brucella ovis has been experienced, several protocols were tested to establish the most reliable method for detection of antibody in indirect enzyme immunoassay. An early version of the assay gave a performance index (PI ¼ sum of optimum percent sensitivity and percent specificity, determined by receiver operator characteristic analysis) of 198.6. This assay used RLPS from B. ovis as the antigen and a monoclonal antibody specific for bovine IgG1 heavy chain-enzyme conjugate for detection. This was not repeatable using other batches of antigen. Newer versions of the assay generally had decreased sensitivity values, giving PIs of 193. Use of a recombinant protein A/G-enzyme conjugate did not improve the PI (PI ¼ 190), giving reduced specificity and higher sensitivity. The final version used B. abortus RB51 RLPS as the antigen and protein A/G-enzyme conjugate for detection, giving a PI of 197. Because of the batch uniformity of the B. abortus RB51 RLPS and the versatility of the protein A/G-enzyme conjugate, the latter version appears to be the most useful for diagnostic serology. |
| format | info:ar-repo/semantics/artículo |
| id | INTA12414 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Taylor & Francis Group |
| publisherStr | Taylor & Francis Group |
| record_format | dspace |
| spelling | INTA124142022-07-27T11:04:28Z Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay Nielsen, Klaus Smith, P. Yu, W. L. Rojas, X. Perez, B. Conde, Sandra Beatriz Samartino, Luis Ernesto Robles, Carlos Alejandro Anticuerpos Brucelosis Brucella Brucella Ovis Técnicas Inmunológicas Antibodies Brucellosis Immunological Techniques Inmunoensayo Because some batch-to-batch variation in the preparation of rough lipopolysaccharide (RLPS) from Brucella ovis has been experienced, several protocols were tested to establish the most reliable method for detection of antibody in indirect enzyme immunoassay. An early version of the assay gave a performance index (PI ¼ sum of optimum percent sensitivity and percent specificity, determined by receiver operator characteristic analysis) of 198.6. This assay used RLPS from B. ovis as the antigen and a monoclonal antibody specific for bovine IgG1 heavy chain-enzyme conjugate for detection. This was not repeatable using other batches of antigen. Newer versions of the assay generally had decreased sensitivity values, giving PIs of 193. Use of a recombinant protein A/G-enzyme conjugate did not improve the PI (PI ¼ 190), giving reduced specificity and higher sensitivity. The final version used B. abortus RB51 RLPS as the antigen and protein A/G-enzyme conjugate for detection, giving a PI of 197. Because of the batch uniformity of the B. abortus RB51 RLPS and the versatility of the protein A/G-enzyme conjugate, the latter version appears to be the most useful for diagnostic serology. Estación Experimental Agropecuaria Bariloche Fil: Nielsen, Klaus. Canadian Food Inspection Agency. Ottawa Laboratories; Canadá Fil: Smith, P. Canadian Food Inspection Agency. Ottawa Laboratories; Canadá Fil: Yu, W. L. Canadian Food Inspection Agency. Ottawa Laboratories; Canadá Fil: Rojas, X. Universidad Austral de Chile. Instituto de Microbiología; Chile Fil: Perez, B. Servicio Agrícola y Ganadero; Chile Fil: Conde, Sandra Beatriz. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural Lobos; Argentina Fil: Samartino, Luis Ernesto. Instituto Nacional de Tecnologia Agropecuaria (INTA); Argentina Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina 2022-07-27T10:56:32Z 2022-07-27T10:56:32Z 2007 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12414 https://www.tandfonline.com/doi/abs/10.1080/15321810701454789 1532-1819 1532-4230 https://doi.org/10.1080/15321810701454789 eng info:eu-repo/semantics/restrictedAccess application/pdf Taylor & Francis Group Journal of Immunoassay and Immunochemistry 28 : 243-250 (2007) |
| spellingShingle | Anticuerpos Brucelosis Brucella Brucella Ovis Técnicas Inmunológicas Antibodies Brucellosis Immunological Techniques Inmunoensayo Nielsen, Klaus Smith, P. Yu, W. L. Rojas, X. Perez, B. Conde, Sandra Beatriz Samartino, Luis Ernesto Robles, Carlos Alejandro Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay |
| title | Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay |
| title_full | Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay |
| title_fullStr | Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay |
| title_full_unstemmed | Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay |
| title_short | Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay |
| title_sort | detection of ovine antibody to brucella ovis by indirect enzyme immunoassay |
| topic | Anticuerpos Brucelosis Brucella Brucella Ovis Técnicas Inmunológicas Antibodies Brucellosis Immunological Techniques Inmunoensayo |
| url | http://hdl.handle.net/20.500.12123/12414 https://www.tandfonline.com/doi/abs/10.1080/15321810701454789 https://doi.org/10.1080/15321810701454789 |
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