RNAi Expression in Cotton for Control of Herbivorous Insects
Cultivated cotton (Gossypium hirsutum) is heavily attacked by various species of insects worldwide and breeding of new varieties resistant to pests is still a hard battle to win. RNAi technology is an important reverse genetics tool to induce gene silencing in eukaryotic organisms and produce phenot...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/parte de libro |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/12411 https://link.springer.com/protocol/10.1007/978-1-0716-1633-8_17 https://doi.org/10.1007/978-1-0716-1633-8_17 |
| _version_ | 1855036751559524352 |
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| author | Salvador, Ricardo Maskin, Laura Niz, José María Turica, Mariana Daniela Pedarros, Analía Soledad Hopp, Horacio Esteban Lewi, Dalia Marcela |
| author_browse | Hopp, Horacio Esteban Lewi, Dalia Marcela Maskin, Laura Niz, José María Pedarros, Analía Soledad Salvador, Ricardo Turica, Mariana Daniela |
| author_facet | Salvador, Ricardo Maskin, Laura Niz, José María Turica, Mariana Daniela Pedarros, Analía Soledad Hopp, Horacio Esteban Lewi, Dalia Marcela |
| author_sort | Salvador, Ricardo |
| collection | INTA Digital |
| description | Cultivated cotton (Gossypium hirsutum) is heavily attacked by various species of insects worldwide and breeding of new varieties resistant to pests is still a hard battle to win. RNAi technology is an important reverse genetics tool to induce gene silencing in eukaryotic organisms and produce phenotypic modifications. In cotton, RNAi was applied to investigate gene function and enhance resistance to insects and pathogens. Different methods and techniques can be used to synthetize double stranded RNA (dsRNA) into plant cells. The Agrobacterium-mediated transformation is a common method to introduce RNAi binary plasmids into cotton genome and obtain stable transgenics plants. This methodology includes the coculture of cotton tissues with Agrobacterium cultures, selection of transgenic cells and induction of somatic embryogenesis to finally obtain transgenic plants after a relatively long period of time. The transient synthesis of dsRNA mediated by virus-induced gene silencing (VIGS) in cotton is an alternative to anticipate the silencing effect of a specific RNA sequence, prior to the development of a stable transgenic plant. VIGS vectors are incorporated into the plant by agroinfiltration technique. During VIGS replication inside plant cells, synthetized dsRNA allows the study on specific heterologous gene expression including the phenotypic effect on herbivorous target pests, thus facilitating a rapid evaluation of dsRNA expressed in cotton plants against individual insect target genes. Here we describe the complementation of these two techniques to evaluate RNAi-based cotton plant protection against insect pests. |
| format | info:ar-repo/semantics/parte de libro |
| id | INTA12411 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA124112022-07-26T23:03:05Z RNAi Expression in Cotton for Control of Herbivorous Insects Salvador, Ricardo Maskin, Laura Niz, José María Turica, Mariana Daniela Pedarros, Analía Soledad Hopp, Horacio Esteban Lewi, Dalia Marcela Gossypium hirsutum Algodón Cotton Biological Control Pest Control Control Biológico Control de Plagas Transgenic Cotton Plants Virus Induced Gene Silencing RNA Interference Cultivated cotton (Gossypium hirsutum) is heavily attacked by various species of insects worldwide and breeding of new varieties resistant to pests is still a hard battle to win. RNAi technology is an important reverse genetics tool to induce gene silencing in eukaryotic organisms and produce phenotypic modifications. In cotton, RNAi was applied to investigate gene function and enhance resistance to insects and pathogens. Different methods and techniques can be used to synthetize double stranded RNA (dsRNA) into plant cells. The Agrobacterium-mediated transformation is a common method to introduce RNAi binary plasmids into cotton genome and obtain stable transgenics plants. This methodology includes the coculture of cotton tissues with Agrobacterium cultures, selection of transgenic cells and induction of somatic embryogenesis to finally obtain transgenic plants after a relatively long period of time. The transient synthesis of dsRNA mediated by virus-induced gene silencing (VIGS) in cotton is an alternative to anticipate the silencing effect of a specific RNA sequence, prior to the development of a stable transgenic plant. VIGS vectors are incorporated into the plant by agroinfiltration technique. During VIGS replication inside plant cells, synthetized dsRNA allows the study on specific heterologous gene expression including the phenotypic effect on herbivorous target pests, thus facilitating a rapid evaluation of dsRNA expressed in cotton plants against individual insect target genes. Here we describe the complementation of these two techniques to evaluate RNAi-based cotton plant protection against insect pests. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Maskin, Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Niz, José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Turica, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Pedarros, Analía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Agrobiotecnología; Argentina Fil: Lewi, Dalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2022-07-26T22:43:52Z 2022-07-26T22:43:52Z 2022 info:ar-repo/semantics/parte de libro info:eu-repo/semantics/bookPart info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12411 https://link.springer.com/protocol/10.1007/978-1-0716-1633-8_17 Salvador, R. et al. (2022). RNAi Expression in Cotton for Control of Herbivorous Insects. pp 217-233. In: Vaschetto, L.M. (eds) RNAi Strategies for Pest Management. Methods in Molecular Biology, vol 2360. Humana, New York, NY 978-1-0716-1633-8 https://doi.org/10.1007/978-1-0716-1633-8_17 eng Methods in Molecular Biology (Springer) info:eu-repo/semantics/restrictedAccess application/pdf Springer RNAi Strategies for Pest Management : methods and protocols / editors: Luis María Vaschetto. New York: Humana, 2021. p. 217–233 |
| spellingShingle | Gossypium hirsutum Algodón Cotton Biological Control Pest Control Control Biológico Control de Plagas Transgenic Cotton Plants Virus Induced Gene Silencing RNA Interference Salvador, Ricardo Maskin, Laura Niz, José María Turica, Mariana Daniela Pedarros, Analía Soledad Hopp, Horacio Esteban Lewi, Dalia Marcela RNAi Expression in Cotton for Control of Herbivorous Insects |
| title | RNAi Expression in Cotton for Control of Herbivorous Insects |
| title_full | RNAi Expression in Cotton for Control of Herbivorous Insects |
| title_fullStr | RNAi Expression in Cotton for Control of Herbivorous Insects |
| title_full_unstemmed | RNAi Expression in Cotton for Control of Herbivorous Insects |
| title_short | RNAi Expression in Cotton for Control of Herbivorous Insects |
| title_sort | rnai expression in cotton for control of herbivorous insects |
| topic | Gossypium hirsutum Algodón Cotton Biological Control Pest Control Control Biológico Control de Plagas Transgenic Cotton Plants Virus Induced Gene Silencing RNA Interference |
| url | http://hdl.handle.net/20.500.12123/12411 https://link.springer.com/protocol/10.1007/978-1-0716-1633-8_17 https://doi.org/10.1007/978-1-0716-1633-8_17 |
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