Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus
Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) represents a strong candidate to develop environmental-friendly pesticides against the fall armyworm (Spodoptera frugiperda), a widespread pest that poses a severe threat to different crops around the world. To date, SfMNPV genomic diversi...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/12408 https://www.sciencedirect.com/science/article/abs/pii/S1567134821000460 https://doi.org/10.1016/j.meegid.2021.104749 |
| _version_ | 1855484967927152640 |
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| author | Masson, Tomás Fabre, María Laura Pidre, Matias Luis Niz, José María Berretta, Marcelo Facundo Romanowski, Víctor Ferrelli, María Leticia |
| author_browse | Berretta, Marcelo Facundo Fabre, María Laura Ferrelli, María Leticia Masson, Tomás Niz, José María Pidre, Matias Luis Romanowski, Víctor |
| author_facet | Masson, Tomás Fabre, María Laura Pidre, Matias Luis Niz, José María Berretta, Marcelo Facundo Romanowski, Víctor Ferrelli, María Leticia |
| author_sort | Masson, Tomás |
| collection | INTA Digital |
| description | Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) represents a strong candidate to develop environmental-friendly pesticides against the fall armyworm (Spodoptera frugiperda), a widespread pest that poses a severe threat to different crops around the world. To date, SfMNPV genomic diversity of different isolates has been mainly studied by means of restriction pattern analyses and by sequencing of the egt region. Here, the genomic diversity present inside an isolate of SfMNPV was explored using high-throughput sequencing for the first time. We identified 704 intrahost single nucleotide variants, from which 184 are nonsynonymous mutations distributed among 82 different coding sequences. We detected several structural variants affecting SfMNPV genome, including two previously reported deletions inside the egt region. A comparative analysis between polymorphisms present in different SfMNPV isolates and our intraisolate diversity data suggests that coding regions with higher genetic diversity are associated with oral infectivity or unknown functions. In this context, through molecular evolution studies we provide evidence of diversifying selection acting on sf29, a putative collagenase which could contribute to the oral infectivity of SfMNPV. Overall, our results contribute to deepen our understanding of the coevolution between SfMNPV and the fall armyworm and will be useful to improve the applicability of this virus as a biological control agent. |
| format | Artículo |
| id | INTA12408 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA124082022-07-26T22:23:25Z Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus Masson, Tomás Fabre, María Laura Pidre, Matias Luis Niz, José María Berretta, Marcelo Facundo Romanowski, Víctor Ferrelli, María Leticia Spodoptera frugiperda Control Biológico Biological Control Pesticides Plaguicidas Baculovirus Genomic Diversity Virus Evolution SfMNPV Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) represents a strong candidate to develop environmental-friendly pesticides against the fall armyworm (Spodoptera frugiperda), a widespread pest that poses a severe threat to different crops around the world. To date, SfMNPV genomic diversity of different isolates has been mainly studied by means of restriction pattern analyses and by sequencing of the egt region. Here, the genomic diversity present inside an isolate of SfMNPV was explored using high-throughput sequencing for the first time. We identified 704 intrahost single nucleotide variants, from which 184 are nonsynonymous mutations distributed among 82 different coding sequences. We detected several structural variants affecting SfMNPV genome, including two previously reported deletions inside the egt region. A comparative analysis between polymorphisms present in different SfMNPV isolates and our intraisolate diversity data suggests that coding regions with higher genetic diversity are associated with oral infectivity or unknown functions. In this context, through molecular evolution studies we provide evidence of diversifying selection acting on sf29, a putative collagenase which could contribute to the oral infectivity of SfMNPV. Overall, our results contribute to deepen our understanding of the coevolution between SfMNPV and the fall armyworm and will be useful to improve the applicability of this virus as a biological control agent. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Masson, Tomás. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); Argentina Fil: Fabre, María Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); Argentina Fil: Pidre, Matias Luis. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); Argentina Fil: Niz, José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Romanowski, Víctor. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); Argentina Fil: Ferrelli, María Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM-UNLP-CONICET); Argentina 2022-07-26T22:16:56Z 2022-07-26T22:16:56Z 2021-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12408 https://www.sciencedirect.com/science/article/abs/pii/S1567134821000460 https://doi.org/10.1016/j.meegid.2021.104749 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Infection, Genetics and Evolution 90 : 104749 (junio 2021) |
| spellingShingle | Spodoptera frugiperda Control Biológico Biological Control Pesticides Plaguicidas Baculovirus Genomic Diversity Virus Evolution SfMNPV Masson, Tomás Fabre, María Laura Pidre, Matias Luis Niz, José María Berretta, Marcelo Facundo Romanowski, Víctor Ferrelli, María Leticia Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus |
| title | Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus |
| title_full | Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus |
| title_fullStr | Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus |
| title_full_unstemmed | Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus |
| title_short | Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus |
| title_sort | genomic diversity in a population of spodoptera frugiperda nucleopolyhedrovirus |
| topic | Spodoptera frugiperda Control Biológico Biological Control Pesticides Plaguicidas Baculovirus Genomic Diversity Virus Evolution SfMNPV |
| url | http://hdl.handle.net/20.500.12123/12408 https://www.sciencedirect.com/science/article/abs/pii/S1567134821000460 https://doi.org/10.1016/j.meegid.2021.104749 |
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