Argentinean Bacillus thuringiensis strains exhibiting distinct morphology of their parasporal crystals
Bacillus thuringiensis is a Gram-positive and sporulated bacterium exhibiting insecticidal activity against a wide range of insects.3 During sporulation, this bacterium produces a number of different proteins forming crystalline inclusions adjacent to the spores (parasporal crystals). Among these in...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Asociación Argentina de Microbiología
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/12391 https://www.sciencedirect.com/science/article/pii/S0325754120300924 https://doi.org/10.1016/j.ram.2020.09.005 |
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| author | Peralta, Cecilia Sauka, Diego Herman Marozzi, Antonela Alejandra del Valle, Eleodoro Eduardo Palma, Leopoldo |
| author_browse | Marozzi, Antonela Alejandra Palma, Leopoldo Peralta, Cecilia Sauka, Diego Herman del Valle, Eleodoro Eduardo |
| author_facet | Peralta, Cecilia Sauka, Diego Herman Marozzi, Antonela Alejandra del Valle, Eleodoro Eduardo Palma, Leopoldo |
| author_sort | Peralta, Cecilia |
| collection | INTA Digital |
| description | Bacillus thuringiensis is a Gram-positive and sporulated bacterium exhibiting insecticidal activity against a wide range of insects.3 During sporulation, this bacterium produces a number of different proteins forming crystalline inclusions adjacent to the spores (parasporal crystals). Among these insecticidal proteins, the most abundant are those commonly known as Cry (Crystal) proteins, which are responsible for exerting a toxic activity (upon ingestion) against insects of different species.5 For this reason, B. thuringiensis has proved to be the most efficient and used bioinsecticide to date.2 However, Spodoptera cosmioides, Spodoptera eridania and Agrotis sp. (Lepidoptera) are species that are not yet controlled by some transgenic crops (e.g. Intacta RR2Pro soybean). Thus, in an attempt to enlarge the host spectrum of this bacterium it is necessary to search for novel strains. In this work we show a sporulated B. thuringiensis Bt-UNVM_84 strain exhibiting a number of rare amorphous to spherical crystal combinations, whereas sporulated B. thuringiensis strain Bt-UNVM-94 showed quasi symmetric bipyramidal parasporal crystals, by using scanning electron microscopy (SEM) (Fig. 1). Strains Bt-UNVM_84 and Bt-UNVM_94 were isolated from Oncativo (Córdoba, Argentina) and Cululú (Santa Fe, Argentina), respectively. The insecticidal activity of these different B. thuringiensis strains is currently under investigation. Each strain was grown in liquid CCY sporulation medium6 for ∼48h (150rpm) until no vegetative cells were observed under a light microscope. The presence of parasporal crystals was first determined using Coomassie blue stained slides1 (1000×) under a Nikon E100 light microscope and confirmed later by a Nikon Ti-Eclipse phase contrast microscope (1000×) (data not shown). For the SEM analysis, aliquots of 1ml were centrifuged for 5minutes (16,000g) at room temperature. Each pellet was washed three times with sterile distilled water and fixed with 100μl 4% formaldehyde. Each fixed preparation was then sent to Centro Integral de Microscopía Electrónica (CIME – CONICET – UNT) for SEM examination. |
| format | info:ar-repo/semantics/artículo |
| id | INTA12391 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Asociación Argentina de Microbiología |
| publisherStr | Asociación Argentina de Microbiología |
| record_format | dspace |
| spelling | INTA123912022-07-25T10:40:30Z Argentinean Bacillus thuringiensis strains exhibiting distinct morphology of their parasporal crystals Cepas argentinas de Bacillus thuringiensis con distinta morfología en sus cristales paraesporales Peralta, Cecilia Sauka, Diego Herman Marozzi, Antonela Alejandra del Valle, Eleodoro Eduardo Palma, Leopoldo Bacillus thuringiensis Bacteria Morfología Argentina Morphology Bacillus thuringiensis is a Gram-positive and sporulated bacterium exhibiting insecticidal activity against a wide range of insects.3 During sporulation, this bacterium produces a number of different proteins forming crystalline inclusions adjacent to the spores (parasporal crystals). Among these insecticidal proteins, the most abundant are those commonly known as Cry (Crystal) proteins, which are responsible for exerting a toxic activity (upon ingestion) against insects of different species.5 For this reason, B. thuringiensis has proved to be the most efficient and used bioinsecticide to date.2 However, Spodoptera cosmioides, Spodoptera eridania and Agrotis sp. (Lepidoptera) are species that are not yet controlled by some transgenic crops (e.g. Intacta RR2Pro soybean). Thus, in an attempt to enlarge the host spectrum of this bacterium it is necessary to search for novel strains. In this work we show a sporulated B. thuringiensis Bt-UNVM_84 strain exhibiting a number of rare amorphous to spherical crystal combinations, whereas sporulated B. thuringiensis strain Bt-UNVM-94 showed quasi symmetric bipyramidal parasporal crystals, by using scanning electron microscopy (SEM) (Fig. 1). Strains Bt-UNVM_84 and Bt-UNVM_94 were isolated from Oncativo (Córdoba, Argentina) and Cululú (Santa Fe, Argentina), respectively. The insecticidal activity of these different B. thuringiensis strains is currently under investigation. Each strain was grown in liquid CCY sporulation medium6 for ∼48h (150rpm) until no vegetative cells were observed under a light microscope. The presence of parasporal crystals was first determined using Coomassie blue stained slides1 (1000×) under a Nikon E100 light microscope and confirmed later by a Nikon Ti-Eclipse phase contrast microscope (1000×) (data not shown). For the SEM analysis, aliquots of 1ml were centrifuged for 5minutes (16,000g) at room temperature. Each pellet was washed three times with sterile distilled water and fixed with 100μl 4% formaldehyde. Each fixed preparation was then sent to Centro Integral de Microscopía Electrónica (CIME – CONICET – UNT) for SEM examination. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Peralta, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina Fil: Marozzi, Antonela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA). Universidad Nacional del Comahue (UNCo); Argentina Fil: del Valle, Eleodoro Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina Fil: Palma, Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina 2022-07-25T10:33:19Z 2022-07-25T10:33:19Z 2021-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12391 https://www.sciencedirect.com/science/article/pii/S0325754120300924 0325-7541 https://doi.org/10.1016/j.ram.2020.09.005 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Asociación Argentina de Microbiología Revista Argentina de Microbiología 53 (4) : 378-379 (Octubre - Diciembre 2021) |
| spellingShingle | Bacillus thuringiensis Bacteria Morfología Argentina Morphology Peralta, Cecilia Sauka, Diego Herman Marozzi, Antonela Alejandra del Valle, Eleodoro Eduardo Palma, Leopoldo Argentinean Bacillus thuringiensis strains exhibiting distinct morphology of their parasporal crystals |
| title | Argentinean Bacillus thuringiensis strains exhibiting distinct morphology of their parasporal crystals |
| title_full | Argentinean Bacillus thuringiensis strains exhibiting distinct morphology of their parasporal crystals |
| title_fullStr | Argentinean Bacillus thuringiensis strains exhibiting distinct morphology of their parasporal crystals |
| title_full_unstemmed | Argentinean Bacillus thuringiensis strains exhibiting distinct morphology of their parasporal crystals |
| title_short | Argentinean Bacillus thuringiensis strains exhibiting distinct morphology of their parasporal crystals |
| title_sort | argentinean bacillus thuringiensis strains exhibiting distinct morphology of their parasporal crystals |
| topic | Bacillus thuringiensis Bacteria Morfología Argentina Morphology |
| url | http://hdl.handle.net/20.500.12123/12391 https://www.sciencedirect.com/science/article/pii/S0325754120300924 https://doi.org/10.1016/j.ram.2020.09.005 |
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