Establishment of a gnotobiotic pig model of Clostridioides difficile infection and disease
Clostridioides difficile (C. difficile) is a gram-positive, spore-forming, anaerobic bacterium known to be the most common cause of hospital-acquired and antibiotic-associated diarrhea. C. difficile infection rates are on the rise worldwide and treatment options are limited, indicating a clear need...
| Main Authors: | , , , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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BioMed Central
2022
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/12350 https://gutpathogens.biomedcentral.com/articles/10.1186/s13099-022-00496-y https://doi.org/10.1186/s13099-022-00496-y |
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| author | Nyblade, Charlotte Parreño, Gladys Viviana Zhou, Peng Hensley, Casey Oakes, Vanessa Mahsoub, Hassan M. Kiley, Kelsey Frazier, Maggie Frazier, Annie Zhang, Yongrong Feng, Hanping Yuan, Lijuan |
| author_browse | Feng, Hanping Frazier, Annie Frazier, Maggie Hensley, Casey Kiley, Kelsey Mahsoub, Hassan M. Nyblade, Charlotte Oakes, Vanessa Parreño, Gladys Viviana Yuan, Lijuan Zhang, Yongrong Zhou, Peng |
| author_facet | Nyblade, Charlotte Parreño, Gladys Viviana Zhou, Peng Hensley, Casey Oakes, Vanessa Mahsoub, Hassan M. Kiley, Kelsey Frazier, Maggie Frazier, Annie Zhang, Yongrong Feng, Hanping Yuan, Lijuan |
| author_sort | Nyblade, Charlotte |
| collection | INTA Digital |
| description | Clostridioides difficile (C. difficile) is a gram-positive, spore-forming, anaerobic bacterium known to be the most common cause of hospital-acquired and antibiotic-associated diarrhea. C. difficile infection rates are on the rise worldwide and treatment options are limited, indicating a clear need for novel therapeutics. Gnotobiotic piglets are an excellent model to reproduce the acute pseudomembranous colitis (PMC) caused by C. difficile due to their physiological similarities to humans and high susceptibility to infection. Here, we established a gnotobiotic pig model of C. difficile infection and disease using a hypervirulent strain. C. difficile-infected pigs displayed classic signs of C. difficile infection, including severe diarrhea and weight loss. Inoculated pigs had severe gross and microscopic intestinal lesions. C. difficile infection caused an increase in pro-inflammatory cytokines in samples of serum, large intestinal contents, and pleural effusion. C. difficile spores and toxins were detected in the feces of inoculated animals as tested by anaerobic culture and cytotoxicity assays. Successful establishment of this model is key for future work as therapeutics can be evaluated in an environment that accurately mimics what happens in humans. The model is especially suitable for evaluating potential prophylactics and therapeutics, including vaccines and passive immune strategies. |
| format | info:ar-repo/semantics/artículo |
| id | INTA12350 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | BioMed Central |
| publisherStr | BioMed Central |
| record_format | dspace |
| spelling | INTA123502022-07-19T16:36:39Z Establishment of a gnotobiotic pig model of Clostridioides difficile infection and disease Nyblade, Charlotte Parreño, Gladys Viviana Zhou, Peng Hensley, Casey Oakes, Vanessa Mahsoub, Hassan M. Kiley, Kelsey Frazier, Maggie Frazier, Annie Zhang, Yongrong Feng, Hanping Yuan, Lijuan Gnotobiotic Animals Swine Animales Notobióticos Cerdo Clostridioides difficile Pseudomembranous colitis Colitis pseudomembranosa Clostridioides difficile (C. difficile) is a gram-positive, spore-forming, anaerobic bacterium known to be the most common cause of hospital-acquired and antibiotic-associated diarrhea. C. difficile infection rates are on the rise worldwide and treatment options are limited, indicating a clear need for novel therapeutics. Gnotobiotic piglets are an excellent model to reproduce the acute pseudomembranous colitis (PMC) caused by C. difficile due to their physiological similarities to humans and high susceptibility to infection. Here, we established a gnotobiotic pig model of C. difficile infection and disease using a hypervirulent strain. C. difficile-infected pigs displayed classic signs of C. difficile infection, including severe diarrhea and weight loss. Inoculated pigs had severe gross and microscopic intestinal lesions. C. difficile infection caused an increase in pro-inflammatory cytokines in samples of serum, large intestinal contents, and pleural effusion. C. difficile spores and toxins were detected in the feces of inoculated animals as tested by anaerobic culture and cytotoxicity assays. Successful establishment of this model is key for future work as therapeutics can be evaluated in an environment that accurately mimics what happens in humans. The model is especially suitable for evaluating potential prophylactics and therapeutics, including vaccines and passive immune strategies. Instituto de Virología Fil: Nyblade, Charlotte. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Parreño, Gladys Viviana. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Zhou, Peng. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Hensley, Casey. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Oakes, Vanessa. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Mahsoub, Hassan M. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Mahsoub, Hassan M. Virginia Polytechnic Institute and State University. Center for Emerging, Zoonotic, and Arthropod‑Borne Pathogens; Estados Unidos Fil: Kiley, Kelsey. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Frazier, Maggie. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Frazier, Annie. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Zhang, Yongrong. University of Maryland at Baltimore. Department of Microbial Pathogenesis; Estados Unidos Fil: Feng, Hanping. University of Maryland at Baltimore. Department of Microbial Pathogenesis; Estados Unidos Fil: Yuan, Lijuan. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Yuan, Lijuan. Virginia Polytechnic Institute and State University. Center for Emerging, Zoonotic, and Arthropod‑Borne Pathogens; Estados Unidos 2022-07-19T16:32:40Z 2022-07-19T16:32:40Z 2022-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12350 https://gutpathogens.biomedcentral.com/articles/10.1186/s13099-022-00496-y 1757-4749 https://doi.org/10.1186/s13099-022-00496-y eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BioMed Central Gut Pathogens 14 : 22 (Junio 2022) |
| spellingShingle | Gnotobiotic Animals Swine Animales Notobióticos Cerdo Clostridioides difficile Pseudomembranous colitis Colitis pseudomembranosa Nyblade, Charlotte Parreño, Gladys Viviana Zhou, Peng Hensley, Casey Oakes, Vanessa Mahsoub, Hassan M. Kiley, Kelsey Frazier, Maggie Frazier, Annie Zhang, Yongrong Feng, Hanping Yuan, Lijuan Establishment of a gnotobiotic pig model of Clostridioides difficile infection and disease |
| title | Establishment of a gnotobiotic pig model of Clostridioides difficile infection and disease |
| title_full | Establishment of a gnotobiotic pig model of Clostridioides difficile infection and disease |
| title_fullStr | Establishment of a gnotobiotic pig model of Clostridioides difficile infection and disease |
| title_full_unstemmed | Establishment of a gnotobiotic pig model of Clostridioides difficile infection and disease |
| title_short | Establishment of a gnotobiotic pig model of Clostridioides difficile infection and disease |
| title_sort | establishment of a gnotobiotic pig model of clostridioides difficile infection and disease |
| topic | Gnotobiotic Animals Swine Animales Notobióticos Cerdo Clostridioides difficile Pseudomembranous colitis Colitis pseudomembranosa |
| url | http://hdl.handle.net/20.500.12123/12350 https://gutpathogens.biomedcentral.com/articles/10.1186/s13099-022-00496-y https://doi.org/10.1186/s13099-022-00496-y |
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