First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
Spittlebugs cause major pasture damage in the Neotropics. As most xylem-feeders, they depend on microbial symbionts to supply essential amino acids to their diet. Here, the obligate nutritional endosymbiont ‘Candidatus Sulcia muelleri’ (Bacteroidetes) was detected in three main cercopid pests of Sou...
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| Format: | Artículo |
| Language: | Inglés |
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Alma Mater Studiorum, Università di Bologna
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/12347 http://www.bulletinofinsectology.org/pdfarticles/vol75-2022-015-020foieri.pdf |
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| author | Foieri, Alvaro Decker Franco, Cecilia Marino de Remes Lenicov, Ana María Arneodo Larochette, Joel Demian |
| author_browse | Arneodo Larochette, Joel Demian Decker Franco, Cecilia Foieri, Alvaro Marino de Remes Lenicov, Ana María |
| author_facet | Foieri, Alvaro Decker Franco, Cecilia Marino de Remes Lenicov, Ana María Arneodo Larochette, Joel Demian |
| author_sort | Foieri, Alvaro |
| collection | INTA Digital |
| description | Spittlebugs cause major pasture damage in the Neotropics. As most xylem-feeders, they depend on microbial symbionts to supply essential amino acids to their diet. Here, the obligate nutritional endosymbiont ‘Candidatus Sulcia muelleri’ (Bacteroidetes) was detected in three main cercopid pests of South America: Notozulia entreriana (Berg), Deois (Deois) mourei Cavichioli et Sakakibara and Deois (Deois) knoblauchii (Berg) (Cercopidae Ishnorhininae). In all insect species, bacteriomes were located laterally in the abdomen, and ultrathin sections of N. entreriana bacteriocytes showed typical sulcia-like bacteria. PCR and sequencing of a
914-bp fragment of the bacterial 16S rRNA gene revealed 100% nucleotide identity among sulcia strains obtained from the three host species. These sequences were also identical to those previously obtained from two other New World spittlebugs of the same subfamily, providing evidence for host/symbiont coevolution. Microscopic and molecular analyses suggested that N. entreriana
lacked additional symbionts (i. e. ‘Candidatus Zinderia insecticola’ or sodalis-like bacteria [Proteobacteria]) that often co-occur with sulcia within members of the superfamily Cercopoidea. Though amplicons were occasionally generated from D. (D.) mourei and D. (D.) knoblauchii with primers intended for zinderia, they failed to sequence. Further research is needed to elucidate the identity of bacteria other than sulcia in Deois spp. |
| format | Artículo |
| id | INTA12347 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Alma Mater Studiorum, Università di Bologna |
| publisherStr | Alma Mater Studiorum, Università di Bologna |
| record_format | dspace |
| spelling | INTA123472022-07-19T16:10:39Z First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii Foieri, Alvaro Decker Franco, Cecilia Marino de Remes Lenicov, Ana María Arneodo Larochette, Joel Demian Bacteria Cercopidae Symbionts Phylogeny South America Simbiontico Filogenia América del Sur Bacteriome Candidatus Sulcia muelleri Spittlebugs cause major pasture damage in the Neotropics. As most xylem-feeders, they depend on microbial symbionts to supply essential amino acids to their diet. Here, the obligate nutritional endosymbiont ‘Candidatus Sulcia muelleri’ (Bacteroidetes) was detected in three main cercopid pests of South America: Notozulia entreriana (Berg), Deois (Deois) mourei Cavichioli et Sakakibara and Deois (Deois) knoblauchii (Berg) (Cercopidae Ishnorhininae). In all insect species, bacteriomes were located laterally in the abdomen, and ultrathin sections of N. entreriana bacteriocytes showed typical sulcia-like bacteria. PCR and sequencing of a 914-bp fragment of the bacterial 16S rRNA gene revealed 100% nucleotide identity among sulcia strains obtained from the three host species. These sequences were also identical to those previously obtained from two other New World spittlebugs of the same subfamily, providing evidence for host/symbiont coevolution. Microscopic and molecular analyses suggested that N. entreriana lacked additional symbionts (i. e. ‘Candidatus Zinderia insecticola’ or sodalis-like bacteria [Proteobacteria]) that often co-occur with sulcia within members of the superfamily Cercopoidea. Though amplicons were occasionally generated from D. (D.) mourei and D. (D.) knoblauchii with primers intended for zinderia, they failed to sequence. Further research is needed to elucidate the identity of bacteria other than sulcia in Deois spp. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Foieri, Alvaro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina Fil: Foieri, Alvaro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Marino de Remes Lenicov, Ana Maria. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Entomología; Argentina Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina 2022-07-19T15:37:27Z 2022-07-19T15:37:27Z 2022-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12347 http://www.bulletinofinsectology.org/pdfarticles/vol75-2022-015-020foieri.pdf 2283-0332 1721-8861 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Alma Mater Studiorum, Università di Bologna Bulletin of Insectology 75 (1) : 15-20 (junio 2022) |
| spellingShingle | Bacteria Cercopidae Symbionts Phylogeny South America Simbiontico Filogenia América del Sur Bacteriome Candidatus Sulcia muelleri Foieri, Alvaro Decker Franco, Cecilia Marino de Remes Lenicov, Ana María Arneodo Larochette, Joel Demian First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii |
| title | First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii |
| title_full | First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii |
| title_fullStr | First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii |
| title_full_unstemmed | First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii |
| title_short | First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii |
| title_sort | first identification of bacterial endosymbionts in three south american spittlebug pests notozulia entreriana deois mourei and deois knoblauchii |
| topic | Bacteria Cercopidae Symbionts Phylogeny South America Simbiontico Filogenia América del Sur Bacteriome Candidatus Sulcia muelleri |
| url | http://hdl.handle.net/20.500.12123/12347 http://www.bulletinofinsectology.org/pdfarticles/vol75-2022-015-020foieri.pdf |
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