Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis
Propolis with a botanic origin in Zuccagnia punctata Cav. (Fabaceae), a plant species widely distributed in the Argentinean Monte region, was used to produce a standardized propolis extract by biological activity and chemical composition. In this paper the effect on microorganisms isolated from otit...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Sage Publishing
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/12342 https://journals.sagepub.com/doi/abs/10.1177/1934578X1801300214 https://doi.org/10.1177/1934578X1801300214 |
| _version_ | 1855036739082518528 |
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| author | Salas, Ana Lilia Zampini, Iris Catiana Maldonado, Luis Maria Isla, María Inés |
| author_browse | Isla, María Inés Maldonado, Luis Maria Salas, Ana Lilia Zampini, Iris Catiana |
| author_facet | Salas, Ana Lilia Zampini, Iris Catiana Maldonado, Luis Maria Isla, María Inés |
| author_sort | Salas, Ana Lilia |
| collection | INTA Digital |
| description | Propolis with a botanic origin in Zuccagnia punctata Cav. (Fabaceae), a plant species widely distributed in the Argentinean Monte region, was used to produce a standardized propolis extract by biological activity and chemical composition. In this paper the effect on microorganisms isolated from otitis was determined. The extract was active against different Staphylococcus strains with MIC and MBC values (75 and 150 μg DE/mL, 200 and 600 μg DE/mL, respectively) and with MIC values of 800 μg DE/mL against Proteus mirabilis. A pharmaceutical product, ear drops containing this extract as bioactive was developed. Stability of ear drops was evaluated for 6 months, and their biological activity, physical and phytochemical properties were preserved. Ear drops showed inhibitory activity on pro-inflammatory enzymes such as lipoxygenase (IC50 values 90 and 100 μg DE/mL), free radical-scavenging effect (SC50 values 23 and 30 μg DE/mL), antihelmintic (LC50 values 70 and 71 μg DE/mL), anti-candida (400 μg DE/mL) and antibacterial activity against Gram-positive bacteria (200 μg DE/mL) at zero time and during six month-storage, respectively. The content of two chalcones, chemical markers of Zuccagnia -type propolis was quantified and its level was maintained while stored at room temperature. Its toxicity was also assayed. Our results are interesting since the extract and ear drops of Zuccagnia -type propolis developed could be promising for use in alternative medicine or phytomedicine as antibacterial, anti-candida, antihelmintic, anti-inflammatory and antioxidant. |
| format | info:ar-repo/semantics/artículo |
| id | INTA12342 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Sage Publishing |
| publisherStr | Sage Publishing |
| record_format | dspace |
| spelling | INTA123422022-07-18T13:42:52Z Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis Salas, Ana Lilia Zampini, Iris Catiana Maldonado, Luis Maria Isla, María Inés Propóleos Calcona Propiedades Medicinales Fabaceae Extractos Vegetales Propolis Chalcones Medicinal Properties Plant Extracts Zuccagnia Zuccagnia punctata Propolis with a botanic origin in Zuccagnia punctata Cav. (Fabaceae), a plant species widely distributed in the Argentinean Monte region, was used to produce a standardized propolis extract by biological activity and chemical composition. In this paper the effect on microorganisms isolated from otitis was determined. The extract was active against different Staphylococcus strains with MIC and MBC values (75 and 150 μg DE/mL, 200 and 600 μg DE/mL, respectively) and with MIC values of 800 μg DE/mL against Proteus mirabilis. A pharmaceutical product, ear drops containing this extract as bioactive was developed. Stability of ear drops was evaluated for 6 months, and their biological activity, physical and phytochemical properties were preserved. Ear drops showed inhibitory activity on pro-inflammatory enzymes such as lipoxygenase (IC50 values 90 and 100 μg DE/mL), free radical-scavenging effect (SC50 values 23 and 30 μg DE/mL), antihelmintic (LC50 values 70 and 71 μg DE/mL), anti-candida (400 μg DE/mL) and antibacterial activity against Gram-positive bacteria (200 μg DE/mL) at zero time and during six month-storage, respectively. The content of two chalcones, chemical markers of Zuccagnia -type propolis was quantified and its level was maintained while stored at room temperature. Its toxicity was also assayed. Our results are interesting since the extract and ear drops of Zuccagnia -type propolis developed could be promising for use in alternative medicine or phytomedicine as antibacterial, anti-candida, antihelmintic, anti-inflammatory and antioxidant. EEA Famaillá Fil: Salas, Ana Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Bioprospección y Fisiología Vegetal; Argentina Fil: Salas, Ana Lilia. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal; Argentina Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Bioprospección y Fisiología Vegetal; Argentina Fil: Zampini, Iris Catiana. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal; Argentina Fil: Zampini, Iris Catiana. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina Fil: Maldonado, Luis Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Isla, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Bioprospección y Fisiología Vegetal; Argentina Fil: Isla, María Inés. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal; Argentina Fil: Isla, María Inés. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina 2022-07-18T13:39:43Z 2022-07-18T13:39:43Z 2018-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12342 https://journals.sagepub.com/doi/abs/10.1177/1934578X1801300214 1934-578X 1555-9475 https://doi.org/10.1177/1934578X1801300214 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Sage Publishing Natural Product Communications 13 (2) : 167-170 (February 2018) |
| spellingShingle | Propóleos Calcona Propiedades Medicinales Fabaceae Extractos Vegetales Propolis Chalcones Medicinal Properties Plant Extracts Zuccagnia Zuccagnia punctata Salas, Ana Lilia Zampini, Iris Catiana Maldonado, Luis Maria Isla, María Inés Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis |
| title | Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis |
| title_full | Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis |
| title_fullStr | Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis |
| title_full_unstemmed | Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis |
| title_short | Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis |
| title_sort | development of a bioproduct for medicinal use with extracts of zuccagnia type propolis |
| topic | Propóleos Calcona Propiedades Medicinales Fabaceae Extractos Vegetales Propolis Chalcones Medicinal Properties Plant Extracts Zuccagnia Zuccagnia punctata |
| url | http://hdl.handle.net/20.500.12123/12342 https://journals.sagepub.com/doi/abs/10.1177/1934578X1801300214 https://doi.org/10.1177/1934578X1801300214 |
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