Occurrence and characterization of a severe isolate of Watermelon mosaic virus from Argentina
More than 50 viruses have been reported in cucurbit crops worldwide. In Argentina, cucurbit viruses have been associated with important yield losses. The most prevalent and widespread potyvirus is Watermelon mosaic virus (WMV). WMV was detected in Argentina in all cucurbit species with high incidenc...
| Autores principales: | , , , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1234 https://link.springer.com/content/pdf/10.1007%2Fs10658-016-0904-z.pdf https://doi.org/10.1007/s10658-016-0904-z |
| _version_ | 1855034770238472192 |
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| author | Perotto, Maria Cecilia Celli, Marcos Giovani Pozzi, Elizabeth Alicia Luciani, Cecilia Elizabeth Conci, Vilma Cecilia |
| author_browse | Celli, Marcos Giovani Conci, Vilma Cecilia Luciani, Cecilia Elizabeth Perotto, Maria Cecilia Pozzi, Elizabeth Alicia |
| author_facet | Perotto, Maria Cecilia Celli, Marcos Giovani Pozzi, Elizabeth Alicia Luciani, Cecilia Elizabeth Conci, Vilma Cecilia |
| author_sort | Perotto, Maria Cecilia |
| collection | INTA Digital |
| description | More than 50 viruses have been reported in cucurbit crops worldwide. In Argentina, cucurbit viruses have been associated with important yield losses. The most prevalent and widespread potyvirus is Watermelon mosaic virus (WMV). WMV was detected in Argentina in all cucurbit species with high incidence. In this study, a WMV isolate (WMV 1 SDE FF) was obtained from a naturally infected squash associated with a severe outbreak on melon and squash crops in an important cucurbit growing area in Santiago del Estero province (Argentina), during a survey conducted in November 2012. The fully sequenced WMV 1 SDE FF genome consists of 10,027 nucleotides and shares 96 % nt identity and 98 % aa identity with the French isolates JF273464.1|C07–014 and EU660581.1|FMF00-LL1 of the WMV molecular group 3. Using the recombination detection program RDP4, two statistically significant recombination events were identified: event 1, an 830-nt long recombinant fragment in the putative P1 coding region, and event 2, a 4071-nt recombinant fragment detected across the HC Pro, P3 and CI coding regions. The putative parental sequences detected for event 1 were the EU660586.1| FBR04–37 (major parent) and JF273468.1|C07–284 (minor parent), both from France. Putative parental sequences for event 2 were JX079685.1| WMV-ShanXi (major parent) and HQ384216.1|Dendrobium (minor parent), from China and USA, respectively. To our knowledge, this is the first complete genome of an Argentine WMV isolate. Our results provide evidence that WMV 1 SDE FF is the causal agent of the strong outbreak reported in melon and squash fields in recent years. |
| format | info:eu-repo/semantics/article |
| id | INTA1234 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA12342022-11-09T11:26:53Z Occurrence and characterization of a severe isolate of Watermelon mosaic virus from Argentina Perotto, Maria Cecilia Celli, Marcos Giovani Pozzi, Elizabeth Alicia Luciani, Cecilia Elizabeth Conci, Vilma Cecilia Enfermedades de las Plantas Sandía Watermelons Plant Diseases Virus del Mosaico de la Sandía Virus de las Plantas Plant Viruses More than 50 viruses have been reported in cucurbit crops worldwide. In Argentina, cucurbit viruses have been associated with important yield losses. The most prevalent and widespread potyvirus is Watermelon mosaic virus (WMV). WMV was detected in Argentina in all cucurbit species with high incidence. In this study, a WMV isolate (WMV 1 SDE FF) was obtained from a naturally infected squash associated with a severe outbreak on melon and squash crops in an important cucurbit growing area in Santiago del Estero province (Argentina), during a survey conducted in November 2012. The fully sequenced WMV 1 SDE FF genome consists of 10,027 nucleotides and shares 96 % nt identity and 98 % aa identity with the French isolates JF273464.1|C07–014 and EU660581.1|FMF00-LL1 of the WMV molecular group 3. Using the recombination detection program RDP4, two statistically significant recombination events were identified: event 1, an 830-nt long recombinant fragment in the putative P1 coding region, and event 2, a 4071-nt recombinant fragment detected across the HC Pro, P3 and CI coding regions. The putative parental sequences detected for event 1 were the EU660586.1| FBR04–37 (major parent) and JF273468.1|C07–284 (minor parent), both from France. Putative parental sequences for event 2 were JX079685.1| WMV-ShanXi (major parent) and HQ384216.1|Dendrobium (minor parent), from China and USA, respectively. To our knowledge, this is the first complete genome of an Argentine WMV isolate. Our results provide evidence that WMV 1 SDE FF is the causal agent of the strong outbreak reported in melon and squash fields in recent years. Inst. Patología Vegetal Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pozzi, Elizabeth Alicia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Luciani, Cecilia E. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-09-18T12:58:45Z 2017-09-18T12:58:45Z 2016 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1234 https://link.springer.com/content/pdf/10.1007%2Fs10658-016-0904-z.pdf 0929-1873 (Print) 1573-8469 (Online) https://doi.org/10.1007/s10658-016-0904-z eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Argentina (nation) European Journal of Plant Pathology 146 (1) : 213–218 (September 2016) |
| spellingShingle | Enfermedades de las Plantas Sandía Watermelons Plant Diseases Virus del Mosaico de la Sandía Virus de las Plantas Plant Viruses Perotto, Maria Cecilia Celli, Marcos Giovani Pozzi, Elizabeth Alicia Luciani, Cecilia Elizabeth Conci, Vilma Cecilia Occurrence and characterization of a severe isolate of Watermelon mosaic virus from Argentina |
| title | Occurrence and characterization of a severe isolate of Watermelon mosaic virus from Argentina |
| title_full | Occurrence and characterization of a severe isolate of Watermelon mosaic virus from Argentina |
| title_fullStr | Occurrence and characterization of a severe isolate of Watermelon mosaic virus from Argentina |
| title_full_unstemmed | Occurrence and characterization of a severe isolate of Watermelon mosaic virus from Argentina |
| title_short | Occurrence and characterization of a severe isolate of Watermelon mosaic virus from Argentina |
| title_sort | occurrence and characterization of a severe isolate of watermelon mosaic virus from argentina |
| topic | Enfermedades de las Plantas Sandía Watermelons Plant Diseases Virus del Mosaico de la Sandía Virus de las Plantas Plant Viruses |
| url | http://hdl.handle.net/20.500.12123/1234 https://link.springer.com/content/pdf/10.1007%2Fs10658-016-0904-z.pdf https://doi.org/10.1007/s10658-016-0904-z |
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