Phytochrome-dependent temperature perception modulates isoprenoid metabolism
Changes in environmental temperature influence many aspects of plant metabolism; however, the underlying regulatory mechanisms remain poorly understood. In addition to their role in light perception, phytochromes (PHYs) have been recently recognized as temperature sensors affecting plant growth. In...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
American Society of Plant Biologists
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/12287 https://academic.oup.com/plphys/article/183/3/869/6116626 https://doi.org/10.1104/pp.20.00019 |
| _version_ | 1855484944613113856 |
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| author | Bianchetti, Ricardo De Luca, Belen De Haro, Luis Alejandro Rosado, Daniele Demarco, Diego Conte, Mariana Bermudez Salazar, Luisa Freschi, Luciano Fernie, Alisdair R. Michaelson, Louise V. Haslam, Richard P. Rossi, Magdalena Carrari, Fernando |
| author_browse | Bermudez Salazar, Luisa Bianchetti, Ricardo Carrari, Fernando Conte, Mariana De Haro, Luis Alejandro De Luca, Belen Demarco, Diego Fernie, Alisdair R. Freschi, Luciano Haslam, Richard P. Michaelson, Louise V. Rosado, Daniele Rossi, Magdalena |
| author_facet | Bianchetti, Ricardo De Luca, Belen De Haro, Luis Alejandro Rosado, Daniele Demarco, Diego Conte, Mariana Bermudez Salazar, Luisa Freschi, Luciano Fernie, Alisdair R. Michaelson, Louise V. Haslam, Richard P. Rossi, Magdalena Carrari, Fernando |
| author_sort | Bianchetti, Ricardo |
| collection | INTA Digital |
| description | Changes in environmental temperature influence many aspects of plant metabolism; however, the underlying regulatory mechanisms remain poorly understood. In addition to their role in light perception, phytochromes (PHYs) have been recently recognized as temperature sensors affecting plant growth. In particular, in Arabidopsis (Arabidopsis thaliana), high temperature reversibly inactivates PHYB, reducing photomorphogenesis-dependent responses. Here, we show the role of phytochrome-dependent temperature perception in modulating the accumulation of isoprenoid-derived compounds in tomato (Solanum lycopersicum) leaves and fruits. The growth of tomato plants under contrasting temperature regimes revealed that high temperatures resulted in coordinated up-regulation of chlorophyll catabolic genes, impairment of chloroplast biogenesis, and reduction of carotenoid synthesis in leaves in a PHYB1B2-dependent manner. Furthermore, by assessing a triple phyAB1B2 mutant and fruit-specific PHYA- or PHYB2-silenced plants, we demonstrated that biosynthesis of the major tomato fruit carotenoid, lycopene, is sensitive to fruit-localized PHY-dependent temperature perception. The collected data provide compelling evidence concerning the impact of PHY-mediated temperature perception on plastid metabolism in both leaves and fruit, specifically on the accumulation of isoprenoid-derived compounds. |
| format | Artículo |
| id | INTA12287 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | American Society of Plant Biologists |
| publisherStr | American Society of Plant Biologists |
| record_format | dspace |
| spelling | INTA122872022-07-11T10:20:34Z Phytochrome-dependent temperature perception modulates isoprenoid metabolism Bianchetti, Ricardo De Luca, Belen De Haro, Luis Alejandro Rosado, Daniele Demarco, Diego Conte, Mariana Bermudez Salazar, Luisa Freschi, Luciano Fernie, Alisdair R. Michaelson, Louise V. Haslam, Richard P. Rossi, Magdalena Carrari, Fernando Carotenoids Chlorophylls Chloroplasts Isoprenoids Phytochrome Temperature Tomatoes Carotenoides Clorofilas Cloroplasto Isoprenoides Fitocroma Temperatura Solanum lycopersicum Tomate Changes in environmental temperature influence many aspects of plant metabolism; however, the underlying regulatory mechanisms remain poorly understood. In addition to their role in light perception, phytochromes (PHYs) have been recently recognized as temperature sensors affecting plant growth. In particular, in Arabidopsis (Arabidopsis thaliana), high temperature reversibly inactivates PHYB, reducing photomorphogenesis-dependent responses. Here, we show the role of phytochrome-dependent temperature perception in modulating the accumulation of isoprenoid-derived compounds in tomato (Solanum lycopersicum) leaves and fruits. The growth of tomato plants under contrasting temperature regimes revealed that high temperatures resulted in coordinated up-regulation of chlorophyll catabolic genes, impairment of chloroplast biogenesis, and reduction of carotenoid synthesis in leaves in a PHYB1B2-dependent manner. Furthermore, by assessing a triple phyAB1B2 mutant and fruit-specific PHYA- or PHYB2-silenced plants, we demonstrated that biosynthesis of the major tomato fruit carotenoid, lycopene, is sensitive to fruit-localized PHY-dependent temperature perception. The collected data provide compelling evidence concerning the impact of PHY-mediated temperature perception on plastid metabolism in both leaves and fruit, specifically on the accumulation of isoprenoid-derived compounds. Instituto de Biotecnología Fil: Bianchetti, Ricardo. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil Fil: De Luca, Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: De Haro, Luis Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Rosado, Daniele. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil Fil: Demarco, Diego. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil Fil: Conte, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Conte, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bermudez Salazar, Luisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Bermudez Salazar, Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bermudez Salazar, Luisa. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Genética; Argentina Fil: Freschi, Luciano. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil Fil: Fernie, Alisdair R. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Michaelson, Louise V. Rothamsted Research. Department of Plant Sciences; Reino Unido Fil: Haslam, Richard P. Rothamsted Research. Department of Plant Sciences; Reino Unido Fil: Rossi, Magdalena. Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica; Brasil Fil: Carrari, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Carrari, Fernando. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Genética; Argentina 2022-07-11T10:12:26Z 2022-07-11T10:12:26Z 2020-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12287 https://academic.oup.com/plphys/article/183/3/869/6116626 1532-2548 https://doi.org/10.1104/pp.20.00019 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Society of Plant Biologists Plant Physiology 183 (3) : 869-882 (Julio 2020) |
| spellingShingle | Carotenoids Chlorophylls Chloroplasts Isoprenoids Phytochrome Temperature Tomatoes Carotenoides Clorofilas Cloroplasto Isoprenoides Fitocroma Temperatura Solanum lycopersicum Tomate Bianchetti, Ricardo De Luca, Belen De Haro, Luis Alejandro Rosado, Daniele Demarco, Diego Conte, Mariana Bermudez Salazar, Luisa Freschi, Luciano Fernie, Alisdair R. Michaelson, Louise V. Haslam, Richard P. Rossi, Magdalena Carrari, Fernando Phytochrome-dependent temperature perception modulates isoprenoid metabolism |
| title | Phytochrome-dependent temperature perception modulates isoprenoid metabolism |
| title_full | Phytochrome-dependent temperature perception modulates isoprenoid metabolism |
| title_fullStr | Phytochrome-dependent temperature perception modulates isoprenoid metabolism |
| title_full_unstemmed | Phytochrome-dependent temperature perception modulates isoprenoid metabolism |
| title_short | Phytochrome-dependent temperature perception modulates isoprenoid metabolism |
| title_sort | phytochrome dependent temperature perception modulates isoprenoid metabolism |
| topic | Carotenoids Chlorophylls Chloroplasts Isoprenoids Phytochrome Temperature Tomatoes Carotenoides Clorofilas Cloroplasto Isoprenoides Fitocroma Temperatura Solanum lycopersicum Tomate |
| url | http://hdl.handle.net/20.500.12123/12287 https://academic.oup.com/plphys/article/183/3/869/6116626 https://doi.org/10.1104/pp.20.00019 |
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