Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina
The objective of this work was to identify, by molecular analysis, the species of the genus Helicoverpa in corn, soybean, and lucerne Bt and non-Bt crops, in central Argentina. A total of 1,343 carterpillars were analyzed by PCR-RFLP. Helicoverpa zea was the only species detected on non-Bt and on mo...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Embrapa Secretaria de Pesquisa e Desenvolvimento
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/12261 https://www.scielo.br/j/pab/a/yJWqGg7F6bXWZdMYn7S4NWN/ https://doi.org/10.1590/S1678-3921.pab2022.v57.02317 |
| _version_ | 1855036725330444288 |
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| author | Balbi, Emilia Ines Flores, Fernando Miguel Tosto, Daniela Sandra Arneodo Larochette, Joel Demian |
| author_browse | Arneodo Larochette, Joel Demian Balbi, Emilia Ines Flores, Fernando Miguel Tosto, Daniela Sandra |
| author_facet | Balbi, Emilia Ines Flores, Fernando Miguel Tosto, Daniela Sandra Arneodo Larochette, Joel Demian |
| author_sort | Balbi, Emilia Ines |
| collection | INTA Digital |
| description | The objective of this work was to identify, by molecular analysis, the species of the genus Helicoverpa in corn, soybean, and lucerne Bt and non-Bt crops, in central Argentina. A total of 1,343 carterpillars were analyzed by PCR-RFLP. Helicoverpa zea was the only species detected on non-Bt and on most Bt corn hybrids. The species H. gelotopoeon predominated on non-Bt soybean and on lucerne, and H. zea larvae occurred in low proportion. Bt soybean did not show Helicoverpa spp. infestations. The exotic H. armigera was not found, though adult moths of this species were light-trapped in a complementary sampling. |
| format | info:ar-repo/semantics/artículo |
| id | INTA12261 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Embrapa Secretaria de Pesquisa e Desenvolvimento |
| publisherStr | Embrapa Secretaria de Pesquisa e Desenvolvimento |
| record_format | dspace |
| spelling | INTA122612022-07-07T10:44:59Z Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina Identificação molecular de Helicoverpa spp. em culturas Bt e não Bt no centro da Argentina Balbi, Emilia Ines Flores, Fernando Miguel Tosto, Daniela Sandra Arneodo Larochette, Joel Demian Helicoverpa zea Helicoverpa armigera PCR Control Biológico RFLP Argentina Biological Control Restriction Fragment Length Polymorphism Helicoverpa gelotopoeon The objective of this work was to identify, by molecular analysis, the species of the genus Helicoverpa in corn, soybean, and lucerne Bt and non-Bt crops, in central Argentina. A total of 1,343 carterpillars were analyzed by PCR-RFLP. Helicoverpa zea was the only species detected on non-Bt and on most Bt corn hybrids. The species H. gelotopoeon predominated on non-Bt soybean and on lucerne, and H. zea larvae occurred in low proportion. Bt soybean did not show Helicoverpa spp. infestations. The exotic H. armigera was not found, though adult moths of this species were light-trapped in a complementary sampling. O objetivo deste trabalho foi identificar, por análise molecular, as espécies do gênero Helicoverpa nas lavouras – Bt e não Bt – de milho, soja e alfafa, no centro da Argentina. Um total de 1.343 lagartas foram analisadas por PCR-RFLP. A espécie H. zea foi a única detectada no milho não Bt e na maioria do Bt. A espécie H. gelotopoeon predominou na soja não Bt e na alfafa, mas H. zea ocorreu em baixa proporção. A soja Bt não apresentou infestações por Helicoverpa spp. A exótica H. armigera não foi encontrada, porém, mariposas adultas dessa espécie foram capturadas com armadilha luminosa em uma amostragem complementar. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Balbi, Emilia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Flores, Fernando Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Tosto, Daniela Sandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2022-07-07T10:30:16Z 2022-07-07T10:30:16Z 2022-06-24 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12261 https://www.scielo.br/j/pab/a/yJWqGg7F6bXWZdMYn7S4NWN/ 1678-3921 0100-204X https://doi.org/10.1590/S1678-3921.pab2022.v57.02317 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Embrapa Secretaria de Pesquisa e Desenvolvimento Pesquisa Agropecuária Brasileira 57 : e02317 (junio 2022) |
| spellingShingle | Helicoverpa zea Helicoverpa armigera PCR Control Biológico RFLP Argentina Biological Control Restriction Fragment Length Polymorphism Helicoverpa gelotopoeon Balbi, Emilia Ines Flores, Fernando Miguel Tosto, Daniela Sandra Arneodo Larochette, Joel Demian Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina |
| title | Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina |
| title_full | Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina |
| title_fullStr | Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina |
| title_full_unstemmed | Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina |
| title_short | Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina |
| title_sort | molecular identification for helicoverpa spp in bt and non bt crops in central argentina |
| topic | Helicoverpa zea Helicoverpa armigera PCR Control Biológico RFLP Argentina Biological Control Restriction Fragment Length Polymorphism Helicoverpa gelotopoeon |
| url | http://hdl.handle.net/20.500.12123/12261 https://www.scielo.br/j/pab/a/yJWqGg7F6bXWZdMYn7S4NWN/ https://doi.org/10.1590/S1678-3921.pab2022.v57.02317 |
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