Halotolerant native bacteria Enterobacter 64S1 and Pseudomonas 42P4 alleviate saline stress in tomato plants
Salinity is one of the principal abiotic stresses that limit the growth and productivity of crops. The use of halotolerant plant growth-promoting rhizobacteria (PGPR) that increase the growth of salt-stressed crops is an environmentally friendly alternative to promote plant yield under salinity. The...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/12259 https://onlinelibrary.wiley.com/doi/10.1111/ppl.13742 https://doi.org/10.1111/ppl.13742 |
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| author | Pérez Rodriguez, María Micaela Pontin, Mariela Ana Piccoli, Patricia Noemí Lobato Ureche, Miguel Andrés Gordillo, María Gabriela Funes Pinter, Mariano Ivan Cohen, Ana Carmen |
| author_browse | Cohen, Ana Carmen Funes Pinter, Mariano Ivan Gordillo, María Gabriela Lobato Ureche, Miguel Andrés Piccoli, Patricia Noemí Pontin, Mariela Ana Pérez Rodriguez, María Micaela |
| author_facet | Pérez Rodriguez, María Micaela Pontin, Mariela Ana Piccoli, Patricia Noemí Lobato Ureche, Miguel Andrés Gordillo, María Gabriela Funes Pinter, Mariano Ivan Cohen, Ana Carmen |
| author_sort | Pérez Rodriguez, María Micaela |
| collection | INTA Digital |
| description | Salinity is one of the principal abiotic stresses that limit the growth and productivity of crops. The use of halotolerant plant growth-promoting rhizobacteria (PGPR) that increase the growth of salt-stressed crops is an environmentally friendly alternative to promote plant yield under salinity. The aim of this study was to test native PGPR, isolated according to their tolerance to NaCl, and to evaluate their influence on morphological, physiological, and biochemical traits promoted by salt stress in tomato plants. Enterobacter 64S1 and Pseudomonas 42P4 were selected as the most efficient strains in terms of salt tolerance. Both strains were classified as moderately resistant to salinity (NaCl) and maintained their plant growth-promoting activities, such as nitrogen fixation and phosphate solubilization, even in the presence of high levels of salt. The results of a greenhouse experiment demonstrated that PGPR inoculation increased root and shoot dry weight, stem diameter, plant height, and leaf area compared to control non-inoculated plants under non-saline stress conditions, reversing the effects of salinity. Inoculated plants showed increased tolerance to salt conditions by reducing electrolyte leakage (improved membrane stability) and lipid peroxidation and increasing chlorophyll quantum efficiency (Fv/Fm) and the performance index. Also, inoculation increased the accumulation of proline and antioxidant non-enzymatic compounds, such as carotenes and total phenolic compounds. The catalase and peroxidase activities increased with salinity, but the effect was reversed by Enterobacter 64S1. In conclusion, Enterobacter 64S1 and Pseudomonas 42P4 isolated from salt-affected regions have the potential to alleviate the deleterious effects of salt stress in tomato crops. |
| format | info:ar-repo/semantics/artículo |
| id | INTA12259 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA122592022-07-06T12:30:41Z Halotolerant native bacteria Enterobacter 64S1 and Pseudomonas 42P4 alleviate saline stress in tomato plants Pérez Rodriguez, María Micaela Pontin, Mariela Ana Piccoli, Patricia Noemí Lobato Ureche, Miguel Andrés Gordillo, María Gabriela Funes Pinter, Mariano Ivan Cohen, Ana Carmen Tomate Halotropismo Estrés Osmótico Salinidad Tomatoes Bacteria Enterobacter Pseudomonas Halotropism Osmotic Stress Salinity Estrés Salino Salinity is one of the principal abiotic stresses that limit the growth and productivity of crops. The use of halotolerant plant growth-promoting rhizobacteria (PGPR) that increase the growth of salt-stressed crops is an environmentally friendly alternative to promote plant yield under salinity. The aim of this study was to test native PGPR, isolated according to their tolerance to NaCl, and to evaluate their influence on morphological, physiological, and biochemical traits promoted by salt stress in tomato plants. Enterobacter 64S1 and Pseudomonas 42P4 were selected as the most efficient strains in terms of salt tolerance. Both strains were classified as moderately resistant to salinity (NaCl) and maintained their plant growth-promoting activities, such as nitrogen fixation and phosphate solubilization, even in the presence of high levels of salt. The results of a greenhouse experiment demonstrated that PGPR inoculation increased root and shoot dry weight, stem diameter, plant height, and leaf area compared to control non-inoculated plants under non-saline stress conditions, reversing the effects of salinity. Inoculated plants showed increased tolerance to salt conditions by reducing electrolyte leakage (improved membrane stability) and lipid peroxidation and increasing chlorophyll quantum efficiency (Fv/Fm) and the performance index. Also, inoculation increased the accumulation of proline and antioxidant non-enzymatic compounds, such as carotenes and total phenolic compounds. The catalase and peroxidase activities increased with salinity, but the effect was reversed by Enterobacter 64S1. In conclusion, Enterobacter 64S1 and Pseudomonas 42P4 isolated from salt-affected regions have the potential to alleviate the deleterious effects of salt stress in tomato crops. EEA La Consulta Fil: Pérez Rodriguez, María Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Pérez Rodriguez, María Micaela. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Pontin, Mariela Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Piccoli, Patricia Noemí. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Lobato Ureche, Miguel Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Lobato Ureche, Miguel Andrés. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Gordillo, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Gordillo, María Gabriela. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Funes Pinter, Mariano Ivan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Fil: Cohen, Ana Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Cohen, Ana Carmen. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina 2022-07-06T12:23:25Z 2022-07-06T12:23:25Z 2022-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/12259 https://onlinelibrary.wiley.com/doi/10.1111/ppl.13742 0031-9317 1399-3054 https://doi.org/10.1111/ppl.13742 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Physiologia Plantarum : e13742 (First published: 30 June 2022) |
| spellingShingle | Tomate Halotropismo Estrés Osmótico Salinidad Tomatoes Bacteria Enterobacter Pseudomonas Halotropism Osmotic Stress Salinity Estrés Salino Pérez Rodriguez, María Micaela Pontin, Mariela Ana Piccoli, Patricia Noemí Lobato Ureche, Miguel Andrés Gordillo, María Gabriela Funes Pinter, Mariano Ivan Cohen, Ana Carmen Halotolerant native bacteria Enterobacter 64S1 and Pseudomonas 42P4 alleviate saline stress in tomato plants |
| title | Halotolerant native bacteria Enterobacter 64S1 and Pseudomonas 42P4 alleviate saline stress in tomato plants |
| title_full | Halotolerant native bacteria Enterobacter 64S1 and Pseudomonas 42P4 alleviate saline stress in tomato plants |
| title_fullStr | Halotolerant native bacteria Enterobacter 64S1 and Pseudomonas 42P4 alleviate saline stress in tomato plants |
| title_full_unstemmed | Halotolerant native bacteria Enterobacter 64S1 and Pseudomonas 42P4 alleviate saline stress in tomato plants |
| title_short | Halotolerant native bacteria Enterobacter 64S1 and Pseudomonas 42P4 alleviate saline stress in tomato plants |
| title_sort | halotolerant native bacteria enterobacter 64s1 and pseudomonas 42p4 alleviate saline stress in tomato plants |
| topic | Tomate Halotropismo Estrés Osmótico Salinidad Tomatoes Bacteria Enterobacter Pseudomonas Halotropism Osmotic Stress Salinity Estrés Salino |
| url | http://hdl.handle.net/20.500.12123/12259 https://onlinelibrary.wiley.com/doi/10.1111/ppl.13742 https://doi.org/10.1111/ppl.13742 |
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