Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina
Broad bean (Vicia faba L) is the fourth most important pulse crop in the world. In Argentina, broad bean production was of 1,841 hectares and 16,500 tons during the 2017 growing season. Broad bean is commonly used in rotations; especially by farmers located in “green belts” that are peri-urban ar...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
BioRxiv
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/12257 https://www.biorxiv.org/content/10.1101/606384v1 https://doi.org/10.1101/606384 |
| _version_ | 1855036723113754624 |
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| author | Rodriguez Pardina, Patricia Nome Docampo, Claudia Reyna, Pablo Gastón Muñoz, Nacira Belen Arguello Caro, Evangelina Beatriz Luque, Andres Vicente Debat, Humberto Julio |
| author_browse | Arguello Caro, Evangelina Beatriz Debat, Humberto Julio Luque, Andres Vicente Muñoz, Nacira Belen Nome Docampo, Claudia Reyna, Pablo Gastón Rodriguez Pardina, Patricia |
| author_facet | Rodriguez Pardina, Patricia Nome Docampo, Claudia Reyna, Pablo Gastón Muñoz, Nacira Belen Arguello Caro, Evangelina Beatriz Luque, Andres Vicente Debat, Humberto Julio |
| author_sort | Rodriguez Pardina, Patricia |
| collection | INTA Digital |
| description | Broad bean (Vicia faba L) is the fourth most important pulse crop in the world. In Argentina, broad
bean production was of 1,841 hectares and 16,500 tons during the 2017 growing season. Broad bean
is commonly used in rotations; especially by farmers located in “green belts” that are peri-urban
areas surrounding large cities that include horticultural family farms. Plants showing marked foliar
mosaic symptoms, typical of viral infection, were collected during the 2015 growing season in the
green belt of Córdoba city, Argentina. Preparations of symptomatic tissues were mechanically
inoculated onto healthy broad bean plants in the greenhouse, which developed symptoms similar to
those observed in the field. In addition, symptomatic samples were positive when tested by indirect
ELISA with the anti-potyvirus group monoclonal antibody. Further, flexuous filamentous particles
typical of potyviruses were observed under the electronic microscope on dip preparations. Lastly,
total RNA was extracted from a symptomatic leaf and high-throughput sequenced, which allowed
the assembly of a single virus sequence corresponding to a new highly divergent strain of Bean
yellow mosaic virus (BYMV). Phylogenetic insights clustered this Argentinean broad bean isolate
(BYMV-ARGbb) within group IX of BYMV. Given the economical importance of this virus and its
associated disease, the results presented here are a pivotal first step oriented to explore the eventual
incidence and epidemiological parameters of BYMV in broad bean in Argentina. |
| format | info:ar-repo/semantics/artículo |
| id | INTA12257 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | BioRxiv |
| publisherStr | BioRxiv |
| record_format | dspace |
| spelling | INTA122572022-07-06T11:43:04Z Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina Rodriguez Pardina, Patricia Nome Docampo, Claudia Reyna, Pablo Gastón Muñoz, Nacira Belen Arguello Caro, Evangelina Beatriz Luque, Andres Vicente Debat, Humberto Julio Vicia Faba Cordoba (Argentina) Argentina Potyvirus Bean Yellow Mosaic Virus Virions High-Throughput Sequencing Virus Mosaico Amarillo del Frijol Virión Secuenciación de Alto Rendimiento Virus Particles Next Generation Sequencing Cinturón Verde de Córdoba Broad bean (Vicia faba L) is the fourth most important pulse crop in the world. In Argentina, broad bean production was of 1,841 hectares and 16,500 tons during the 2017 growing season. Broad bean is commonly used in rotations; especially by farmers located in “green belts” that are peri-urban areas surrounding large cities that include horticultural family farms. Plants showing marked foliar mosaic symptoms, typical of viral infection, were collected during the 2015 growing season in the green belt of Córdoba city, Argentina. Preparations of symptomatic tissues were mechanically inoculated onto healthy broad bean plants in the greenhouse, which developed symptoms similar to those observed in the field. In addition, symptomatic samples were positive when tested by indirect ELISA with the anti-potyvirus group monoclonal antibody. Further, flexuous filamentous particles typical of potyviruses were observed under the electronic microscope on dip preparations. Lastly, total RNA was extracted from a symptomatic leaf and high-throughput sequenced, which allowed the assembly of a single virus sequence corresponding to a new highly divergent strain of Bean yellow mosaic virus (BYMV). Phylogenetic insights clustered this Argentinean broad bean isolate (BYMV-ARGbb) within group IX of BYMV. Given the economical importance of this virus and its associated disease, the results presented here are a pivotal first step oriented to explore the eventual incidence and epidemiological parameters of BYMV in broad bean in Argentina. Instituto de Patología Vegetal Fil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Reyna, Pablo Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Reyna, Pablo Gastón. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Muñoz, Nacira Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Muñoz, Nacira Belén. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Exactas Físicas y Naturales (FCEFyN).Cátedra de Fisiología Vegetal; Argentina Fil: Arguello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Arguello Caro, Evangelina Beatriz. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias (FCA). Cátedra de Zoología Agrícola,; Argentina Fil: Luque, Andres Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Luque, Andres Vicente. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2022-07-06T11:30:34Z 2022-07-06T11:30:34Z 2019-04-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion http://hdl.handle.net/20.500.12123/12257 https://www.biorxiv.org/content/10.1101/606384v1 2692-8205 https://doi.org/10.1101/606384 eng info:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola. info:eu-repograntAgreement/INTA/PNHFA-1106075/AR./Desarrollo de bases tecnológicas para el aumento de la competitividad con sostenibilidad de las Legumbres en Argentina. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BioRxiv BioRxiv : the preprint server for biology. (April 11, 2019) |
| spellingShingle | Vicia Faba Cordoba (Argentina) Argentina Potyvirus Bean Yellow Mosaic Virus Virions High-Throughput Sequencing Virus Mosaico Amarillo del Frijol Virión Secuenciación de Alto Rendimiento Virus Particles Next Generation Sequencing Cinturón Verde de Córdoba Rodriguez Pardina, Patricia Nome Docampo, Claudia Reyna, Pablo Gastón Muñoz, Nacira Belen Arguello Caro, Evangelina Beatriz Luque, Andres Vicente Debat, Humberto Julio Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina |
| title | Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina |
| title_full | Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina |
| title_fullStr | Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina |
| title_full_unstemmed | Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina |
| title_short | Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina |
| title_sort | bean yellow mosaic virus infecting broad bean in the green belt of cordoba argentina |
| topic | Vicia Faba Cordoba (Argentina) Argentina Potyvirus Bean Yellow Mosaic Virus Virions High-Throughput Sequencing Virus Mosaico Amarillo del Frijol Virión Secuenciación de Alto Rendimiento Virus Particles Next Generation Sequencing Cinturón Verde de Córdoba |
| url | http://hdl.handle.net/20.500.12123/12257 https://www.biorxiv.org/content/10.1101/606384v1 https://doi.org/10.1101/606384 |
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