Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance
The sunflower (Helianthus annuus) transcription factor HaHB11 (H. annuus Homeobox 11) belongs to the homeodomain-leucine zipper family and confers improved yield to maize (Zea mays) hybrids (HiII × B73) and lines. Here we report that transgenic maize lines expressing HaHB11 exhibited better performa...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Oxford University Press
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/12217 https://academic.oup.com/plphys/article-abstract/189/1/230/6527217 https://doi.org/10.1093/plphys/kiac054 |
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| author | Raineri, Jesica Caraballo, Luciano Rigalli, Nicolás Portapila, Margarita Otegui, María Elena Lía Chan, Raquel |
| author_browse | Caraballo, Luciano Lía Chan, Raquel Otegui, María Elena Portapila, Margarita Raineri, Jesica Rigalli, Nicolás |
| author_facet | Raineri, Jesica Caraballo, Luciano Rigalli, Nicolás Portapila, Margarita Otegui, María Elena Lía Chan, Raquel |
| author_sort | Raineri, Jesica |
| collection | INTA Digital |
| description | The sunflower (Helianthus annuus) transcription factor HaHB11 (H. annuus Homeobox 11) belongs to the homeodomain-leucine zipper family and confers improved yield to maize (Zea mays) hybrids (HiII × B73) and lines. Here we report that transgenic maize lines expressing HaHB11 exhibited better performance under waterlogging, both in greenhouse and field trials carried out during three growth cycles. Transgenic plants had increased chlorophyll content, wider stems, more nodal roots, greater total aerial biomass, a higher harvest index, and increased plant grain yield. Under severe defoliation caused by a windstorm during flowering, transgenic genotypes were able to set more grains than controls. This response was confirmed in controlled defoliation assays. Hybrids generated by crossing B73 HaHB11 lines with the contrasting Mo17 lines were also tested in the field and exhibited the same beneficial traits as the parental lines, compared with their respective controls. Moreover, they were less penalized by stress than commercial hybrids. Waterlogging tolerance increased via improvement of the root system, including more xylem vessels, reduced tissue damage, less superoxide accumulation, and altered carbohydrate metabolism. Multivariate analyses corroborated the robustness of the differential traits observed. Furthermore, canopy spectral reflectance data, computing 29 vegetation indices associated with biomass, chlorophyll, and abiotic stress, helped to distinguish genotypes as well as their growing conditions. Altogether the results reported here indicate that this sunflower gene constitutes a suitable tool to improve maize plants for environments prone to waterlogging and/or wind defoliation. |
| format | info:ar-repo/semantics/artículo |
| id | INTA12217 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | INTA122172022-07-01T15:51:45Z Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance Raineri, Jesica Caraballo, Luciano Rigalli, Nicolás Portapila, Margarita Otegui, María Elena Lía Chan, Raquel Girasol Maíz Rendimiento de Cultivos Mejoramiento Genético Genetic Improvement Defoliación Genotipos Sunflowers Maize Crop Yield Defoliation Genotypes The sunflower (Helianthus annuus) transcription factor HaHB11 (H. annuus Homeobox 11) belongs to the homeodomain-leucine zipper family and confers improved yield to maize (Zea mays) hybrids (HiII × B73) and lines. Here we report that transgenic maize lines expressing HaHB11 exhibited better performance under waterlogging, both in greenhouse and field trials carried out during three growth cycles. Transgenic plants had increased chlorophyll content, wider stems, more nodal roots, greater total aerial biomass, a higher harvest index, and increased plant grain yield. Under severe defoliation caused by a windstorm during flowering, transgenic genotypes were able to set more grains than controls. This response was confirmed in controlled defoliation assays. Hybrids generated by crossing B73 HaHB11 lines with the contrasting Mo17 lines were also tested in the field and exhibited the same beneficial traits as the parental lines, compared with their respective controls. Moreover, they were less penalized by stress than commercial hybrids. Waterlogging tolerance increased via improvement of the root system, including more xylem vessels, reduced tissue damage, less superoxide accumulation, and altered carbohydrate metabolism. Multivariate analyses corroborated the robustness of the differential traits observed. Furthermore, canopy spectral reflectance data, computing 29 vegetation indices associated with biomass, chlorophyll, and abiotic stress, helped to distinguish genotypes as well as their growing conditions. Altogether the results reported here indicate that this sunflower gene constitutes a suitable tool to improve maize plants for environments prone to waterlogging and/or wind defoliation. EEA Pergamino Fil: Raineri, Jesica. Universidad Nacional del Litoral-CONICET. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Caraballo, Luciano. Universidad Nacional del Litoral-CONICET. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Rigalli, Nicolás. Universidad Nacional de Rosario—CONICET. CIFASIS; Argentina Fil: Portapila, Margarita. Universidad Nacional de Rosario—CONICET. CIFASIS; Argentina Fil: Otegui, María Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Ecofisiología; Argentina Fil: Otegui, María E. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Otegui, María E. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal; Argentina Fil: Lía Chan, Raquel. Universidad Nacional del Litoral—CONICET. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina 2022-07-01T15:46:04Z 2022-07-01T15:46:04Z 2022-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12217 https://academic.oup.com/plphys/article-abstract/189/1/230/6527217 0032-0889 1532-2548 (online) https://doi.org/10.1093/plphys/kiac054 eng info:eu-repo/semantics/restrictedAccess application/pdf Oxford University Press Plant Physiology 189 (1) : 230-247. (May 2022) |
| spellingShingle | Girasol Maíz Rendimiento de Cultivos Mejoramiento Genético Genetic Improvement Defoliación Genotipos Sunflowers Maize Crop Yield Defoliation Genotypes Raineri, Jesica Caraballo, Luciano Rigalli, Nicolás Portapila, Margarita Otegui, María Elena Lía Chan, Raquel Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance |
| title | Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance |
| title_full | Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance |
| title_fullStr | Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance |
| title_full_unstemmed | Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance |
| title_short | Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance |
| title_sort | expressing the sunflower transcription factor hahb11 in maize improves waterlogging and defoliation tolerance |
| topic | Girasol Maíz Rendimiento de Cultivos Mejoramiento Genético Genetic Improvement Defoliación Genotipos Sunflowers Maize Crop Yield Defoliation Genotypes |
| url | http://hdl.handle.net/20.500.12123/12217 https://academic.oup.com/plphys/article-abstract/189/1/230/6527217 https://doi.org/10.1093/plphys/kiac054 |
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